肿瘤坏死因子-α单克隆抗体干预矽肺纤维化的实验研究

发布时间：2019-06-27 07:50

【摘要】：目的观察肿瘤坏死因子-α(TNF-α)单克隆抗体(英夫利西)对矽尘诱导的大鼠肺纤维化的抑制作用,探讨英夫利西治疗肺纤维化的可能机制。方法将48只雄性Wistar大鼠随机分为3组,每组16只。染尘组和干预组大鼠气管内一次性注入50 mg SiO_2粉尘,次日起干预组给予15 mg/kg英夫利西皮下注射,共5 d,染尘组仅注射等量生理盐水;对照组气管内和皮下均注射生理盐水。各组分别于建模后7 d、14 d处死大鼠各8只,以HE、Masson染色对肺组织炎症和纤维化程度进行评价。ELISA法检测血、支气管肺泡灌洗液(BALF)中TNF-α含量,Western blot检测肺组织NF-κB蛋白表达量。结果英夫利西干预后,肺泡炎评分(7 d)和纤维化评分(14 d)优化;血清TNF-α含量降低,BALF中TNF-α含量仅7 d降低,7 d时NF-κB p65蛋白表达下调,以上结果差异均具有统计学意义(P0.05)。结论TNF-α单克隆抗体可降低TNF-α水平,进而抑制NF-κB信号通路的活化,缓解矽尘诱导的大鼠肺纤维化进程。
[Abstract]:Objective to observe the inhibitory effect of tumor necrosis factor-伪 (TNF- 伪) monoclonal antibody (inflixime) on pulmonary fibrosis induced by silicon dust in rats, and to explore the possible mechanism of inflixime in the treatment of pulmonary fibrosis. Methods 48 male Wistar rats were randomly divided into 3 groups with 16 rats in each group. The rats in the dust group and the intervention group were injected with 50 mg SiO_2 dust at one time. From the next day, the intervention group was given 15 mg/kg inflixime subcutaneous injection for 5 days, the dust exposure group was only injected with the same amount of normal saline, and the control group was injected with normal saline in both trachea and subcutaneous. Eight rats in each group were killed on the 7th day and 14th day after modeling, and the degree of inflammation and fibrosis in lung tissue were evaluated by HE,Masson staining. The content of TNF- 伪 in blood and (BALF) in bronchoalveolar lavage fluid (BALF) was detected by Elisa. The expression of NF- 魏 B protein in lung tissue was detected by Elisa. Results after the intervention, the alveolar score (7 days) and fibrosis score (14 days) were optimized, the serum TNF- 伪 content decreased, the TNF- 伪 content in BALF decreased only 7 days, and the expression of NF- 魏 B p65 protein decreased at 7 days, the above results were statistically significant (P 0.05). Conclusion TNF- 伪 monoclonal antibody can decrease the level of TNF- 伪, inhibit the activation of NF- kappa B signaling pathway and alleviate the process of pulmonary fibrosis induced by silicon dust in rats.
【作者单位】：青岛市中心医疗集团职业病防治院;青岛市中心血站;青岛大学医学院附属医院;
【基金】：青岛市科技发展指导计划项目(KJZD-12-23-nsh)
【分类号】：R135.2

【相似文献】