基于PC12细胞模型分析大豆蛋白水解物对神经元氧化损伤的保护作用

发布时间：2019-06-27 10:05

【摘要】：本文基于PC12细胞模型研究大豆蛋白水解物(Soybean protein isolate hydrolysates,SPIHs)对神经元氧化损伤的保护作用。以大豆蛋白为原料,经过酶解和膜分离得到四种分子量不同的水解物,我们首先检测了SPIHs的抗氧化能力;然后用H2O2刺激P12细胞,建立神经元氧化损伤模型,并以适当浓度的SPIHs处理细胞,通过检测各种生物学指标评价对细胞氧化损伤的保护作用。结果显示,低分子量的SPIHs表现出最强的抗氧化活性;能够提高损伤细胞的存活率和抗氧化酶活力,减少乳酸脱氢酶(LDH)的释放量和丙二醛(MDA)的生成,抑制细胞活性氧(ROS)的累积(p0.05或p0.01),且变化呈现一定的剂量依赖关系。研究认为,低分子量的SPIHs对神经元氧化损伤具有保护作用,可以作为功能性成分用于保护神经元氧化损伤相关的功能食品和保健品的开发。
[Abstract]:The protective effect of soybean protein hydrolysate (Soybean protein isolate hydrolysates,SPIHs on oxidative damage of neurons was studied based on PC12 cell model. Using soybean protein as raw material, four kinds of hydrolysates with different molecular weight were obtained by enzyme hydrolysis and membrane separation, and then the antioxidant capacity of SPIHs was measured, and then the oxidative damage model of neurons was established by stimulating P12 cells with H2O2, and the cells were treated with appropriate concentration of SPIHs to evaluate the protective effect on oxidative damage of P12 cells by testing various biological indexes. The results showed that low molecular weight SPIHs showed the strongest antioxidant activity, which could increase the survival rate and antioxidant enzyme activity of injured cells, reduce the release of lactic dehydrogenase (LDH) and the production of malondialdehyde (MDA), and inhibit the accumulation of reactive oxygen species (ROS) (p0.05 or p0.01) in a dose-dependent manner. It is considered that low molecular weight SPIHs has protective effect on neuronal oxidative damage and can be used as a functional component to protect the development of functional food and health products related to neuronal oxidative damage.
【作者单位】：吉林大学营养与功能食品研究室;
【基金】：国家科技支撑计划项目(2012BAD33B03) 吉林大学研究生创新基金资助项目(2014116)
【分类号】：R151

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