南昌地区儿童手足口病病原型别及其流行特征分析
发布时间：2019-07-02 12:21
【摘要】：目的了解南昌地区儿童手足口病(HFMD)的病原学构成,为该地区HFMD的流行病学防控提供基础。方法收集2014年4至12月临床送检疑似HFMD患者咽拭子标本,运用荧光定量RT-PCR检测肠道病毒通用型(EV)、肠道病毒71型(EV71)和柯萨奇病毒A16型(CVA16)核酸;每季度按照等距抽样的方法选取一定量的非EV71、非CVA16型的其他EV标本,利用VP4区分型引物进行巢式PCR,产物通过测序和Gen Bank Blast比对进行分型鉴定。结果 2014年4至12月共检测到EV阳性病例19 059例,其中EV71、CVA16和其他EV检出率分别为23.22%(4 425/19 059)、11.36%(2 166/19 059)、65.42%(12 468/19 059)。其他EV抽样检测结果显示,第2季度CVA10占比最高,为24.39%(10/41),其次是CVA4和CVA6,各占19.51%(8/41);第3季度CVA6占比最高,为73.08%(95/130),其次是CVA10和CVA4,分别占11.54%(15/130)和6.15%(8/130);而第4季度CVA6占比94.74%(36/38),其次是CVA10占5.26%(2/38)。结论 2014年第2至第4季度非EV71、非CVA16型的其他EV检出率最高,且CVA6为2014年第3和第4季度南昌地区引起HFMD的优势病毒株。
[Abstract]:Objective to investigate the etiological composition of (HFMD) in children with hand, foot and mouth disease (HFMD) in Nanchang area, and to provide a basis for epidemiological prevention and control of HFMD in Nanchang area. Methods from April to December 2014, throat swabs from suspected HFMD patients were collected and the nucleic acids of enterovirus universal (EV), enterovirus 71 (EV71) and Coxsackievirus A16 (CVA16) were detected by fluorescence quantitative RT-PCR (FQ-PCR). According to the method of isometric sampling, a certain number of other EV samples of non-EV71, non-CVA16 type were selected every quarter, and the nesting PCR, products were identified by sequencing and Gen Bank Blast comparison with VP4 discriminant primers. Results from April to December 2014, a total of 19 059 EV positive cases were detected. The positive rates of EV71,CVA16 and other EV were 23.22% (4.425 鈮，
本文编号：2508925
[Abstract]:Objective to investigate the etiological composition of (HFMD) in children with hand, foot and mouth disease (HFMD) in Nanchang area, and to provide a basis for epidemiological prevention and control of HFMD in Nanchang area. Methods from April to December 2014, throat swabs from suspected HFMD patients were collected and the nucleic acids of enterovirus universal (EV), enterovirus 71 (EV71) and Coxsackievirus A16 (CVA16) were detected by fluorescence quantitative RT-PCR (FQ-PCR). According to the method of isometric sampling, a certain number of other EV samples of non-EV71, non-CVA16 type were selected every quarter, and the nesting PCR, products were identified by sequencing and Gen Bank Blast comparison with VP4 discriminant primers. Results from April to December 2014, a total of 19 059 EV positive cases were detected. The positive rates of EV71,CVA16 and other EV were 23.22% (4.425 鈮，
本文编号：2508925
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