食品中病毒提取与检测方法研究进展
发布时间：2019-07-02 14:54
【摘要】：随着食源性疾病事件的频频发生,食品安全已成为一个严重的世界卫生问题。研究表明大多数食源性疾病的暴发主要是由食源性病毒引起的。此类病毒在许多食物表面或者内部都有分布,会引起疫病暴发和流行,危害人体健康,对食品企业造成重大经济损失。为及时了解食品中病毒情况,需要对不同食物来源中的食源性病毒进行准确检测。通常食源性病毒的检测分成3个部分:病毒的提取、病毒基因组的纯化和分子检测。但在实际检测中,由于食物的组成成分差异性大以及食源性病毒污染量少、致病性高等特点,使得不同食品中病毒的检测难度增加。本文就已报道文献中食源性病毒提取及检测方法加以阐述,为进一步完善食品中病毒检测方法提供思路。
[Abstract]:With the frequent occurrence of food-borne disease, food safety has become a serious health problem. Studies have shown that most of the outbreaks of food-borne diseases are primarily caused by food-borne viruses. The virus is distributed on many food surfaces or in the interior, which can cause the outbreak and the epidemic of the epidemic disease, endanger the health of the human body, and cause great economic loss to the food enterprise. In ord to understand that condition of the virus in the food in a timely manner, it is necessary to accurately detect the food-borne virus in different food sources. In general, the detection of the food-borne virus is divided into three parts: the extraction of the virus, the purification of the viral genome, and the molecular detection. But in the actual detection, the detection difficulty of the virus in different foods is increased due to the large difference of the composition components of the food and the small amount of the food-borne virus and the high pathogenicity. In this paper, the method of the extraction and detection of the food-borne virus in the literature has been described, and the thought of the method of virus detection in the food is further improved.
【作者单位】： 浙江省疾病预防控制中心营养与食品安全所;浙江中医药大学;浙江省检验检疫科学技术研究院;
【基金】：浙江省公益性项目(2015C32017) 浙江省医药卫生科技计划项目(骨干人才)(2015RCA003和2017KY282)
【分类号】：R155.5
，
本文编号：2509021
[Abstract]:With the frequent occurrence of food-borne disease, food safety has become a serious health problem. Studies have shown that most of the outbreaks of food-borne diseases are primarily caused by food-borne viruses. The virus is distributed on many food surfaces or in the interior, which can cause the outbreak and the epidemic of the epidemic disease, endanger the health of the human body, and cause great economic loss to the food enterprise. In ord to understand that condition of the virus in the food in a timely manner, it is necessary to accurately detect the food-borne virus in different food sources. In general, the detection of the food-borne virus is divided into three parts: the extraction of the virus, the purification of the viral genome, and the molecular detection. But in the actual detection, the detection difficulty of the virus in different foods is increased due to the large difference of the composition components of the food and the small amount of the food-borne virus and the high pathogenicity. In this paper, the method of the extraction and detection of the food-borne virus in the literature has been described, and the thought of the method of virus detection in the food is further improved.
【作者单位】： 浙江省疾病预防控制中心营养与食品安全所;浙江中医药大学;浙江省检验检疫科学技术研究院;
【基金】：浙江省公益性项目(2015C32017) 浙江省医药卫生科技计划项目(骨干人才)(2015RCA003和2017KY282)
【分类号】：R155.5
，
本文编号：2509021
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