体质性发育迟缓男童邻苯二甲酸酯暴露与线粒体DNA拷贝数及氧化损伤的关联性研究
发布时间：2019-08-26 09:40
【摘要】：[目的]采用病例对照研究方法,观察邻苯二甲酸酯暴露对男童线粒体DNA氧化损伤及线粒体DNA拷贝数的影响,探索邻苯二甲酸酯影响男性性发育的可能机制。[方法]将2013—2014年经上海市某三级甲等医院确诊为体质性发育迟缓的8~15岁男童纳入研究,共48例病例,匹配以同一年龄段同一医院就诊于外科的正常男童60例作为对照。采用高效液相色谱-串联质谱法检测病例和对照男童尿液中邻苯二甲酸单丁酯(Mn BP)、邻苯二甲酸甲酯(MMP)、邻苯二甲酸乙酯(MEP)邻苯二甲酸(2-乙基己基)单酯(MEHP)、邻苯二甲酸(2-乙基-5羟基己基)单酯(MEHHP)、邻苯二甲酸(2-乙基-5氧己基)单酯(MEOHP)浓度。运用t检验及多元线性回归模型分析邻苯二甲酸酯水平与线粒体DNA氧化损伤、线粒体DNA拷贝数在病例和对照中的差异及两者间的关联。[结果]病例组男童尿液Mn BP、MMP、MEHP、Sum DEHP浓度显著高于正常对照组(P值分别为0.017、0.008、0.038、0.020)。病例组男童线粒体DNA拷贝数高于对照组(P=0.001),两组线粒体DNA氧化损伤水平差异无统计学意义。男童尿液MMP、MEHP、Sum DEHP浓度与线粒体DNA拷贝数存在显著正相关(P0.05),但未发现线粒体DNA氧化损伤水平与邻苯二甲酸酯暴露水平间显著关联。[结论]体质性发育迟缓男童体内邻苯二甲酸酯暴露水平和线粒体DNA拷贝数显著升高,邻苯二甲酸酯暴露可能是线粒体功能受损的一个危险因素。
[Abstract]:[objective] to observe the effect of phthalate exposure on oxidative damage of mitochondrial DNA and the number of DNA torture in boys by case-control study, and to explore the possible mechanism of phthalate affecting male sexual development. [methods] A total of 48 boys aged 8 years and 15 years old who were diagnosed as physical retardation by a Grade 3A hospital in Shanghai from 2013 to 2014 were enrolled in the study. 60 normal boys who were treated in surgery in the same hospital of the same age group were used as control. High performance liquid chromatography-tandem mass spectrometry was used to determine the concentration of monobutyl phthalate (Mn BP), methyl phthalate (MMP), ethyl phthalate (MEP) phthalic acid (2-ethylhexyl) monoester (MEHP), phthalic acid (2-ethyl-5-hydroxyhexyl) monoester (MEHHP), phthalic acid (2-ethyl-5-oxohexyl) monoester (MEOHP) in the urine of case and control boys. T test and multiple linear regression model were used to analyze the difference between phthalate level and oxidative damage of mitochondrial DNA, the number of DNA copies in cases and controls, and the correlation between them. [results] the urine Mn BP,MMP,MEHP,Sum DEHP concentration in the case group was significantly higher than that in the normal control group (P = 0.017, 0.008, 0.038, 0.020, respectively). The number of mitochondrial DNA copies in the case group was higher than that in the control group (P 鈮，
本文编号：2529176
[Abstract]:[objective] to observe the effect of phthalate exposure on oxidative damage of mitochondrial DNA and the number of DNA torture in boys by case-control study, and to explore the possible mechanism of phthalate affecting male sexual development. [methods] A total of 48 boys aged 8 years and 15 years old who were diagnosed as physical retardation by a Grade 3A hospital in Shanghai from 2013 to 2014 were enrolled in the study. 60 normal boys who were treated in surgery in the same hospital of the same age group were used as control. High performance liquid chromatography-tandem mass spectrometry was used to determine the concentration of monobutyl phthalate (Mn BP), methyl phthalate (MMP), ethyl phthalate (MEP) phthalic acid (2-ethylhexyl) monoester (MEHP), phthalic acid (2-ethyl-5-hydroxyhexyl) monoester (MEHHP), phthalic acid (2-ethyl-5-oxohexyl) monoester (MEOHP) in the urine of case and control boys. T test and multiple linear regression model were used to analyze the difference between phthalate level and oxidative damage of mitochondrial DNA, the number of DNA copies in cases and controls, and the correlation between them. [results] the urine Mn BP,MMP,MEHP,Sum DEHP concentration in the case group was significantly higher than that in the normal control group (P = 0.017, 0.008, 0.038, 0.020, respectively). The number of mitochondrial DNA copies in the case group was higher than that in the control group (P 鈮，
本文编号：2529176
本文链接：https://www.wllwen.com/yixuelunwen/yufangyixuelunwen/2529176.html
