缺氧诱导骨髓间充质干细胞条件培养基体外修复辐射损伤小肠上皮细胞变化

发布时间：2018-03-21 00:08

本文选题：间充质干细胞　切入点：培养基　出处：《中国组织工程研究》2015年45期 　论文类型：期刊论文

【摘要】：背景:间充质干细胞条件培养基含有丰富的间充质干细胞旁分泌物质,被认为是干细胞移植理想的替代方案。然而,正常状态的间充质干细胞条件培养基可能由于旁分泌活性不足,常无法有效修复损伤组织。目的:观察缺氧诱导的骨髓间充质干细胞条件培养基(MSC-CM~(Hyp))对大鼠小肠隐窝上皮IEC-6细胞辐射损伤后增殖和凋亡的影响,并进一步探讨其修复小肠上皮细胞的旁分泌机制。方法:IEC-6细胞接受10 Gy X射线照射后分别加入MSC-CM~(Hyp)、正常培养的骨髓间充质干细胞条件培养基(MSC-CM~(Nor)组)及DMEM-F12培养基(DMEM-F12组)培养。结果与结论:锥虫蓝染色、流式细胞仪、Western blot检测结果显示,与DMEM-F12组相比,MSC-CM~(Hyp)组IEC-6细胞存活数和增殖率明显增加(P0.05),凋亡率和凋亡相关因子Caspases-3/8表达明显下降(P0.05),而MSC-CM~(Nor)组的各项指标与DMEM-F12组比较差异无显著性意义(P0.05)。EILSA检测结果显示,与MSC-CM~(Nor)相比,MSC-CM~(Hyp)中血管内皮生长因子、碱性成纤维细胞生长因子、胰岛素样生长因子1、和白细胞介素10的含量明显升高(P0.05)。结果表明,缺氧诱导的骨髓间充质干细胞条件培养基中细胞因子含量明显升高,显著促进辐射损伤IEC-6细胞的增殖,同时下调细胞凋亡信号,从而进一步促进损伤细胞的修复。
[Abstract]:Background: the mesenchymal stem cell conditioned medium contains abundant paracrine substances from mesenchymal stem cells and is considered to be an ideal alternative for stem cell transplantation. The normal mesenchymal stem cell conditioned medium may be due to insufficient paracrine activity. Objective: to observe the effect of hypoxia-induced bone marrow mesenchymal stem cell conditioned medium (MSC-CMH) on proliferation and apoptosis of rat intestinal crypt epithelial IEC-6 cells after radiation injury. To further explore the paracrine mechanism of the repair of intestinal epithelial cells. Methods 10 Gy X ray irradiation was given to 10 Gy of small intestinal epithelial cells. The normal cultured bone marrow mesenchymal stem cells were treated with MSC-CMM-F12 medium.) and the DMEM-F12 medium, DMEM-F12 group, respectively, were used to study the paracrine mechanism of the repair of intestinal epithelial cells. Results and conclusions: trypanosoma blue staining, Western blot analysis by flow cytometry showed that, Compared with the DMEM-F12 group, the survival and proliferation rate of IEC-6 cells in the MSC-CMN group increased significantly, while the apoptosis rate and the expression of apoptosis-related factor (Caspases-3/8) decreased significantly. However, there was no significant difference between the MSC-CM-HypHypHypHypHypHypHypHypHypA group and the DMEM-F12 group in the expression of apoptosis and apoptosis-related factors (P0.05. Elsa), there was no significant difference between the MSC-CM-HypHypHypHypA group and the DMEM-F12 group. The contents of vascular endothelial growth factor (VEGF), basic fibroblast growth factor (bFGF), insulin-like growth factor 1 (IGF-1) and interleukin-10 (IL-10) were significantly higher than those in MSC-CMN. Hypoxia induced bone marrow mesenchymal stem cell conditioned medium significantly increased cytokines content, significantly promoted the proliferation of radiation induced IEC-6 cells, and down-regulated the apoptotic signal, thus further promoting the repair of injured cells.
【作者单位】：广东省人民医院消化内科广东省医学科学院;
【基金】：国家自然科学基金项目(81300279) 中国博士后科学基金项目(2012M521579) 广东省自然科学基金项目(S2013040013549) 广东省医学科研基金项目(A2014020) 广东省科技计划项目(2015A020212029)~~
【分类号】：R818

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