Toll样受体5激动剂CBLB502对7.0Gy ~60Coγ射线照射恒河猴的辐射防护作用观察

发布时间：2018-05-02 20:46

本文选题：辐射损伤 + 实验性　；参考：《解放军医学杂志》2015年04期

【摘要】：目的观察Toll样受体5激动剂CBLB502对7.0Gy 60Coγ射线全身照射恒河猴的辐射防护作用。方法 30只健康成年恒河猴分为对症治疗组、WR-2721(辐射防护剂氨磷汀)组和CBLB5022.5、10、40μg/kg组,每组6只。所有动物均用60Coγ射线放射源一次全身双侧照射7.0Gy(剂量率13.00~13.52c Gy/min)。CBLB502给药剂量为2.5、10和40μg/kg,阳性对照药WR-2721剂量为30mg/kg,对症治疗组注射生理盐水0.3ml/kg,均为照射前0.5h一次肌肉注射。所有动物照射后在同一原则下给予对症治疗。观察各组动物一般体征、外周血象、造血祖细胞集落培养情况及组织病理学检查结果。结果照射后40d时CBLB50210、40μg/kg组动物存活率均为100%,而对症治疗组存活率仅为33%(2/6);WR-2721组各系造血恢复稍快于对症治疗组,但差异无统计学意义;与对症治疗组比较,CBLB50240μg/kg组外周血白细胞和血小板最低值显著增高,血小板低值持续时间缩短,开始恢复时间提前,输血量明显减少(P0.05),骨髓、肠道等组织损伤明显减轻。结论 7.0Gy 60Coγ射线全身照射猴均发生了重度骨髓型急性放射病。照前30min一次性给予40μg/kgCBLB502对7.0Gy 60Coγ射线照射猴有明显的辐射防护作用,其效果优于目前常用的辐射防护剂WR-2721。
[Abstract]:Objective to observe the radiation protective effect of Toll like receptor 5 agonist CBLB502 on 7.0Gy 60Co 纬-ray irradiation in rhesus monkeys. Methods Thirty healthy adult rhesus monkeys were divided into two groups: WR-2721 (radiation protector) group and CBLB5022.5 (1040 渭 g/kg) group. All animals were irradiated with 60Co 纬 -ray radiation source at a dose rate of 13.00 ~ 13.52c Gy/min).CBLB502 at a dose rate of 2.5 渭 g / kg and 40 渭 g / kg respectively. The dose of positive control drug WR-2721 was 30 mg / kg. In the symptomatic treatment group, 0.3 ml / kg of normal saline was injected, and 0.5 h before irradiation, all the animals were injected intramuscularly. All animals were given symptomatic treatment under the same principle after irradiation. The general signs, peripheral blood, colony culture of hematopoietic progenitor cells and histopathological findings were observed. Results at 40 days after irradiation, the survival rate of CBLB5021010 渭 g/kg group was 100, while the survival rate of symptomatic treatment group was 33 / 6 / 6. The hematopoiesis recovery of WR-2721 group was slightly faster than that of symptomatic treatment group, but the difference was not statistically significant. Compared with the symptomatic treatment group, the minimum value of peripheral blood leukocytes and platelets in CBLB50240 渭 g/kg group was significantly increased, the duration of platelet low value was shortened, the recovery time was earlier, the amount of blood transfusion was significantly reduced, and the injury of bone marrow and intestinal tract was obviously alleviated. Conclusion all monkeys exposed to 7.0Gy 60Co 纬-ray have severe acute radiation sickness of bone marrow type. Before irradiation, 40 渭 g/kgCBLB502 30min had obvious radiation protection effect on 7.0Gy 60Co 纬 -ray irradiation monkey, which was superior to WR-2721, a commonly used radiation protection agent.
【作者单位】：军事医学科学院放射与辐射医学研究所;
【基金】：全军医学科技重大专项课题(2008ZXJ09001-004)~~
【分类号】：R818

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