运动对不同浓度2，3，7，8-TCDD染毒大鼠附睾功能的影响

发布时间：2018-11-04 09:26

【摘要】：目的:选SD大鼠为实验对象,研究运动对不同剂量2,3,7,8-TCDD染毒大鼠生殖系统的影响,探讨运动干预对2,3,7,8-TCDD的生殖毒性是否有削减作用。方法:将100只8周龄雄性SD大鼠随机分为10组,分别为EC/ET1/ET2/ET3/ET4,NC/NT1/NT2/NT3/NT4。T1(ET1,NT1),T2(ET2,NT2),T3(ET3,NT3),T4(ET4,NT4)组大鼠的首次注射剂量分别为0.4 μ g/kg ·BW,1.6μ g/kg·BW,6.4μg/kg·BW和25.6μ g/kg·BW,之后每周均给予上述剂量的21%作为染毒持续剂量。C(EC,NC)组大鼠注射染毒剂量为0μ g/kg·BW,根据体重比例注射等量玉米油。E组大鼠运动方案为大鼠尾部负重5%自身体重,每次游泳运动30min,每周5天,共持续8周。N组大鼠静养8周。8周后,将所有大鼠处死,测定大鼠体重,附睾重量,附睾脏器质量指数,大鼠血清睾酮(T)、黄体生成素(LH)、卵泡刺激素(FSH)。结果:1.E组大鼠体重非常显著性小于N组(P0.01)。C组显著大于T2、T3、T4组,T1组显著大于T3、T4组,T2、T3组显著大于T4组(P0.01)。2.T4组附睾脏器指数显著大于其余4组,其余四组见间差异不显著。3.N组FSH显著小于E组(P0.01),剂量主效应不显著,运动与否和剂量的交互效应不显著。4.运动主效应显著:N组(安静组)的LH显著小于E组(运动组);剂量主效应显著:T1组显著大于其他各组,T2组显著大于T4组,其余各组间差异不显著。运动与否和剂量的交互作用显著。N组各剂量水平间LH无显著性差异,但E组上T1组显著大于C组和T4组,其余各组间差异不显著。5.运动与否和剂量的交互作用对T的影响效应显著,T2水平上N组(安静组)显著大于E组(运动组)。结论:1.随着TCDD染毒剂量增加,大鼠正常生长发育受抑制的程度更加明显,剂量效应关系明显。2.TCDD染毒扰乱了下丘脑-垂体-性腺轴的正常功能,使此轴上的激素水平异常改变。3.TCDD与游泳训练具有拮抗性,游泳可适度减轻TCDD中毒症状,但只在染毒浓度不超过25.6μg/kg·BW时有效。4.TCDD开始明显在机体中发挥生殖毒性的"阈值"浓度大概在0.4～1.6μg/kg·BW之间。
[Abstract]:Objective: to study the effects of exercise on the reproductive system of rats exposed to different doses of TCDD and to explore whether exercise intervention can reduce the reproductive toxicity of TCDD. Methods: 100 8-week-old male SD rats were randomly divided into 10 groups: EC/ET1/ET2/ET3/ET4,NC/NT1/NT2/NT3/NT4.T1 (ET1,NT1), T2 (ET2,NT2), T3 (ET3,NT3) and T4 (ET4,). The initial injection doses of NT4 group were 0.4 渭 g/kg BW,1.6 渭 g/kg BW,6.4 渭 g/kg BW and 25.6 渭 g/kg BW, respectively. After that, 21% of the above doses were given weekly as the continuous dose of. C (EC,NC). The rats in the group were injected with 0 渭 g/kg BW,. According to the proportion of body weight, the rats in group E were injected with the same amount of corn oil. The rats in group E carried 5% body weight on the tail of the rats, swimming for 30 minutes, 5 days a week for a total of 8 weeks. The rats in group N were treated with rest for 8 weeks. After 8 weeks, all the rats were killed. Body weight, epididymal weight, organ mass index of epididymis and serum testosterone (T), luteinizing hormone (LH),) follicle stimulating hormone (FSH).) were measured in rats. Results: 1. The body weight of group E was significantly lower than that of group N (P0.01). C group was significantly larger than that of T2T3 T4 group, T1 group was significantly larger than T3 T4 group, T2 group. Group T3 was significantly larger than group T4 (P0.01). The organ index of epididymis in 2.T4 group was significantly higher than that in other four groups, but there was no significant difference among the other four groups. 3.The FSH in group N was significantly lower than that in group E (P0.01), and the main dose effect was not significant. The interaction between exercise and dose was not significant. 4. The LH of group N (quiet group) was significantly lower than that of group E (exercise group), and the dose effect was significant: group T1 was significantly larger than other groups, group T2 was significantly larger than group T4, and there was no significant difference among the other groups. The interaction between exercise and dose was significant. There was no significant difference in LH between different dose levels in group N. however, the LH in group E was significantly higher than that in group C and group T4, but there was no significant difference among the other groups. The interaction between exercise and dose had a significant effect on T. Group N (quiet group) was significantly greater than group E (exercise group) at T2 level. Conclusion: 1. With the increase of the dose of TCDD, the inhibition of normal growth and development of rats was more obvious, and the dose-effect relationship was obvious. 2.TCDD treatment disturbed the normal function of hypothalamus-pituitary-gonadal axis. 3.TCDD has antagonistic effect with swimming training. Swimming can alleviate the symptoms of TCDD poisoning moderately. But only when the concentration of 4.TCDD was not more than 25.6 渭 g/kg BW, the threshold concentration of 4.TCDD to produce reproductive toxicity was about 0.4 渭 g/kg BW or 1.6 渭 g/kg BW.
【学位授予单位】：北京体育大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R87

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