抗阻训练对老龄大鼠骨骼肌细胞凋亡和新生细胞核的补偿研究
发布时间：2019-03-03 16:35
【摘要】：目的：抗阻训练对骨骼肌衰老有积极的作用，本实验采取新型尾部抗阻训练方法研究骨骼肌细胞核凋亡和新生情况，为其内部的机制研究提供基础理论依据。同时在新型大鼠抗阻训练的模型下研究其负荷强度水平对机体的影响状况，为老年人健身负荷量提供理论参考。 方法：本实验以18月龄SD健康大鼠32只为研究对象，随机分为4组：安静对照组（C）、小负荷抗阻训练组（L）、中负荷抗阻训练组（M）、大负荷抗阻训练组（H）。分别对L组、M组、H组进行10周的新型尾部抗阻训练干预，所有动物取材进行Brdu（5-溴脱氧尿嘧啶核苷）标记，10周末处死，，取腓肠肌，进行免疫组化实验和生化指标的检测，分析各组腓肠肌细胞核凋亡和新生情况，以及分析各组骨骼肌蛋白总量和MHC异形体mRNA表达状况等。 结果： 1.10周末，与C组相比，各训练组大鼠骨骼肌力量有显著性增加，各训练组腓肠肌蛋白总量也有显著性的升高，L组和M组的α-actin mRNA表达有非常显著性的升高. 2.10周末，与C组相比，L组和M组的MHCI mRNA、MHCIIa mRNA、MHCIIx mRNA显著性升高，L组和M组以及H组的MHCIIb mRNA表达显著性降低，H组的MHCI mRNA、MHCIIxmRNA表达显著性降低。 3.10周末，与C组相比，M组和H组的细胞核凋亡百分比有显著性的升高；与C组相比，L组和M组以及H组腓肠肌新生细胞核百分比有显著性的升高。与C组相比，L组和M组的细胞更替百分比有显著性的降低。 结论 1.10周尾部抗阻训练能使老龄大鼠骨骼肌力量以及蛋白总量增多，且中负荷组效果最佳。 2.10周中、低强度的抗阻训练使老龄大鼠骨骼肌快型和慢型MHC异行体mRNA表达量显著性升高，表现为快型比例相对减少，慢型比例相对增多。 3.10周大、中负荷抗阻训练能引起细胞核凋亡百分比显著性增多，各个训练组新生细胞核数目百分比也有显著性的增多，小、中负荷的细胞核更替百分比显著降低，从而延缓和对抗骨骼肌衰老。
[Abstract]:Aim: resistance training has a positive effect on skeletal muscle senescence. In this study, a new type of tail resistance training method was used to study the apoptosis and neogenesis of skeletal muscle nucleus, and provide the basic theoretical basis for the study of its internal mechanism. At the same time, under the model of resistance training of new type rats, the influence of load intensity on the body was studied, and the theoretical reference was provided for the body-building load of the elderly. Methods: thirty-two 18-month-old healthy SD rats were randomly divided into 4 groups: quiet control group (C), small load resistance training group (L), moderate load resistance training group (M), heavy load resistance training group (H). Group L, group M and group H were treated with new tail resistance training for 10 weeks. All animals were taken and labeled with Brdu (5-bromodeoxyuridine), then sacrificed at the end of 10 weeks, and gastrocnemius muscle was taken out, and the muscle of gastrocnemius was taken from the muscle of gastrocnemius. The apoptosis and neogenesis of gastrocnemius muscle nucleus and the total protein content of skeletal muscle and the expression of MHC heteroform mRNA in each group were analyzed by immunohistochemical assay and biochemical test. Results: 1. At the end of the 10th week, compared with group C, the skeletal muscle strength and the total protein of gastrocnemius muscle in each training group increased significantly. The expression of 伪-actin mRNA in L group and M group was significantly higher than that in group C. 2. At the end of 10 weeks, compared with C group, the expression of MHCI mRNA,MHCIIa mRNA,MHCIIxmRNA in L group and M group was significantly increased, the expression of MHCIIb mRNA in L group, M group and H group was significantly lower than that in H group, and the expression of MHCI mRNA,MHCIIxmRNA in H group was significantly lower than that in H group. 3. At the end of the week, compared with group C, the percentage of nucleus apoptosis in group M and group H was significantly higher than that in group C, and in group L, group M and group H, the percentage of new nuclei in gastrocnemius muscle was significantly higher than that in group C. Compared with C group, the percentage of cell turnover in L group and M group was significantly lower than that in C group. 10 weeks tail resistance training can increase the skeletal muscle strength and total protein of aged rats, and the middle load group has the best effect. 2. 2. In 10 weeks, low-intensity resistance training significantly increased the expression of fast and slow MHC heterozygous mRNA in skeletal muscle of aged rats, which showed that the proportion of fast type and slow type was relatively decreased, while the proportion of slow type was relatively increased. 3. At the age of 10 weeks, the percentage of nucleus apoptosis induced by resistance training in middle load increased significantly, and the percentage of new nuclei in each training group also increased significantly, and the percentage of nucleus replacement in middle load decreased significantly, while the percentage of nucleus replacement in middle load decreased significantly in each training group. Thus delaying and fighting skeletal muscle senescence.
【学位授予单位】：河北师范大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：G804.2
本文编号：2433891
[Abstract]:Aim: resistance training has a positive effect on skeletal muscle senescence. In this study, a new type of tail resistance training method was used to study the apoptosis and neogenesis of skeletal muscle nucleus, and provide the basic theoretical basis for the study of its internal mechanism. At the same time, under the model of resistance training of new type rats, the influence of load intensity on the body was studied, and the theoretical reference was provided for the body-building load of the elderly. Methods: thirty-two 18-month-old healthy SD rats were randomly divided into 4 groups: quiet control group (C), small load resistance training group (L), moderate load resistance training group (M), heavy load resistance training group (H). Group L, group M and group H were treated with new tail resistance training for 10 weeks. All animals were taken and labeled with Brdu (5-bromodeoxyuridine), then sacrificed at the end of 10 weeks, and gastrocnemius muscle was taken out, and the muscle of gastrocnemius was taken from the muscle of gastrocnemius. The apoptosis and neogenesis of gastrocnemius muscle nucleus and the total protein content of skeletal muscle and the expression of MHC heteroform mRNA in each group were analyzed by immunohistochemical assay and biochemical test. Results: 1. At the end of the 10th week, compared with group C, the skeletal muscle strength and the total protein of gastrocnemius muscle in each training group increased significantly. The expression of 伪-actin mRNA in L group and M group was significantly higher than that in group C. 2. At the end of 10 weeks, compared with C group, the expression of MHCI mRNA,MHCIIa mRNA,MHCIIxmRNA in L group and M group was significantly increased, the expression of MHCIIb mRNA in L group, M group and H group was significantly lower than that in H group, and the expression of MHCI mRNA,MHCIIxmRNA in H group was significantly lower than that in H group. 3. At the end of the week, compared with group C, the percentage of nucleus apoptosis in group M and group H was significantly higher than that in group C, and in group L, group M and group H, the percentage of new nuclei in gastrocnemius muscle was significantly higher than that in group C. Compared with C group, the percentage of cell turnover in L group and M group was significantly lower than that in C group. 10 weeks tail resistance training can increase the skeletal muscle strength and total protein of aged rats, and the middle load group has the best effect. 2. 2. In 10 weeks, low-intensity resistance training significantly increased the expression of fast and slow MHC heterozygous mRNA in skeletal muscle of aged rats, which showed that the proportion of fast type and slow type was relatively decreased, while the proportion of slow type was relatively increased. 3. At the age of 10 weeks, the percentage of nucleus apoptosis induced by resistance training in middle load increased significantly, and the percentage of new nuclei in each training group also increased significantly, and the percentage of nucleus replacement in middle load decreased significantly, while the percentage of nucleus replacement in middle load decreased significantly in each training group. Thus delaying and fighting skeletal muscle senescence.
【学位授予单位】：河北师范大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：G804.2
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