优秀龙舟运动员尿液~1H NMR代谢组学研究

发布时间：2019-04-10 20:39

【摘要】：目的:应用1H NMR代谢组学方法对优秀龙舟运动员尿液代谢特征进行分析,结合尿常规进行全面、系统的训练监控。方法:于集训期间及比赛前后连续5次收集运动员晨尿,采用1H NMR的代谢组学方法结合尿常规检测,运用多元统计分析和t检验对实验数据进行分析。结果:(1)与安静时相比,男、女运动员尿液p H值、尿蛋白(PRO)和红细胞(BLD)阳性检出率分别在训练期和比赛后具有显著性变化(P0.05),特别是集训1周和集训2周时。(2)与安静时相比,男运动员在不同训练期和比赛后尿液中乳酸、谷氨酸、丙氨酸、谷氨酰胺、二甲胺和柠檬酸含量均显著增加,而苯乙酰甘氨酸则明显下降(P0.05);女运动员尿液中牛磺酸含量显著增加,苯乙酰甘氨酸同样显著下降(P0.05)。运动员尿液代谢组学指标主要在集训1周、比赛次日和恢复1周时变化明显。不同性别运动员尿液共有7种代谢物含量存在显著性差异(P0.05)。结论:(1)龙舟运动能够引起运动员机体内与糖、氨基酸代谢相关的代谢物发生显著改变,运用1H NMR的代谢组学技术实现对运动员无损伤的训练监控可行。(2)不同性别运动员尿液代谢组学信息存在差异,可能由于训练负荷安排和运动员机能状态不同所致。(3)龙舟运动员尿液代谢组学信息比尿常规信息能更加敏感和全面地反映运动员机能状态,对运动性疲劳和过度训练的监控和早期诊断可能更有优势。
[Abstract]:Aim: to analyze the urine metabolic characteristics of elite dragon boat athletes by 1H NMR metabonomics, and to conduct comprehensive and systematic training monitoring combined with urine routine. Methods: the morning urine of athletes was collected 5 times during the training period and before and after the competition. The metabonomics method of 1H NMR combined with urine routine test was used to analyze the experimental data by multivariate statistical analysis and t test. Results: (1) there were significant changes in urine pH value, urine protein (PRO) and erythrocyte (BLD) positive rate between male and female athletes during training and after competition (P0.05). (2) the contents of lactic acid, glutamic acid, alanine, glutamine, dimethylamine and citric acid in urine of male athletes increased significantly in different training and post-match periods. However, phenylacetyl glycine decreased significantly (P0.05). Taurine content in urine of female athletes increased significantly, and phenylacetyl glycine also decreased significantly (P0.05). The urine metabonomics indexes of athletes mainly changed in one week of training, the second day of competition and one week of recovery. There were significant differences in the contents of seven metabolites in urine of athletes of different sexes (P0.05). Conclusion: (1) Dragon boat exercise can cause significant changes in metabolism of glucose and amino acids in athletes. It is feasible to use the metabonomics technology of 1H NMR to monitor the athletes without injury. (2) there are differences in the information of urine metabonomics among athletes of different sexes. (3) the information of urine metabonomics of dragon boat athletes can reflect the athletes' functional status more sensitively and comprehensively than the routine information of urine. Monitoring and early diagnosis of exercise-induced fatigue and overtraining may be more advantageous.
【作者单位】： 山西大学体育学院;
【基金】：中国博士后科学基金资助(2014M560197,2015T80234) 山西省教育厅“省高校重点实验室”专项基金资助
【分类号】：R87

【参考文献】

中国期刊全文数据库 前4条

1 冒海蕾;徐e，

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