脊髓爆震伤后早期治疗神经元的形态学变化

发布时间：2018-04-14 09:23

本文选题：脊髓爆震伤 + 药物　；参考：《中国矫形外科杂志》2006年16期

【摘要】：[目的]研究脊髓爆震伤后早期采用不同药物干预对脊髓前角运动神经元影响。[方法]将36只家兔随机分为对照组(A组,n=12)、地塞米松实验纽(B组,n=12)、甲强龙实验组(C组,n=12),每组家兔均采用0.9 g单质金属炸药黑索金(cyc lotrim ethylene trin itram ine)进行爆震,伤后各组分别给予静脉输入生理盐水、地塞米松及甲强龙,于伤后6 h、24 h 2个时间点取材,在光镜下观察脊髓前角运动神经元形态和数量的改变。[结果]发现爆震伤后6 h脊髓运动神经元出现可逆性改变,伤后24 h脊髓死亡运动神经元显著增加,伤后早期给予地塞米松及甲强龙治疗后,死亡神经元的数量减少与对照组相比有显著的统计学意义(P0.001),而B、C组之间无显著差别(P0.05)。[结论]脊髓爆震伤后早期应用糖皮质激素对运动神经元具有保护作用,在本实验条件下,甲强龙在早期脊髓爆震伤中的疗效与地塞米松相比没有优势。
[Abstract]:[objective] to study the effects of different drugs on spinal cord anterior horn motoneurons in early stage after spinal cord blast injury.[methods] Thirty-six rabbits were randomly divided into control group (group A), dexamethasone test group (group B) and group C (group C). Each group was detonated with 0.9 g lotrim lotrim ethylene trin itram.Each group was given intravenous infusion of normal saline, dexamethasone and methylenolone respectively. The morphologic and quantitative changes of motor neurons in the anterior horn of spinal cord were observed under light microscope at 24 h after injury.[results] reversible changes were found in spinal cord motor neurons at 6 h after blast injury, and significantly increased at 24 h after injury. After early treatment with dexamethasone and methylenolone, the spinal cord motor neurons were significantly increased.The number of dead neurons decreased significantly compared with the control group (P 0.001), but there was no significant difference between group B and C (P 0.05).[conclusion] early application of glucocorticoid has protective effect on motor neurons after spinal cord blast injury. Under this experimental condition, methylenolone has no advantage over dexamethasone in early spinal cord explosive injury.
【作者单位】：第四军医大学附属西京医院全军骨科研究所第四军医大学附属西京医院全军骨科研究所解放军第359医院骨科第四军医大学附属西京医院全军骨科研究所第四军医大学附属西京医院全军骨科研究所第四军医大学神经生物教研室第四军医大学附属西京医院全军骨科研究所第四军医大学附属西京医院全军骨科研究所
【分类号】：R82

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