犬颅脑枪弹伤后局部脑组织c-fos基因表达

发布时间：2018-04-15 16:27

本文选题：脑损伤 + 创伤　；参考：《中华创伤杂志》2003年07期

【摘要】：目的　探讨犬颅脑枪弹伤后c -fos基因表达及其变化规律 ,为进一步了解火器性颅脑损伤的机制提供实验基础。　方法　以德国小口径步枪子弹致伤犬颅脑贯通伤模型为对象 ,采用免疫组化法检测对照组脑组织及伤后不同时间弹道挫伤区、震荡区及脑干神经元中c -fos基因的表达。　结果　对照组脑皮层神经元中c -fos基因呈弱表达 ,弹道挫伤区、震荡区及脑干神经元中c-fos基因表达于伤后 30min开始增加 ,2h达到高峰 ,3h逐渐下降 ,且c -fos基因表达在弹道震荡区较挫伤区更为明显 (P 0 .0 5 )。　结论　犬脑组织弹道挫伤区、震荡区及脑干神经元中c-fos基因表达是对损伤刺激的早期反应 ,其表达可能是由Leao播散性抑制引起 ,并与细胞内外信号转导和细胞凋亡有关。
[Abstract]:Objective to investigate the C expression of -fos gene in dogs after craniocerebral gunshot injury and its change rule, to provide the experimental basis for further understanding the mechanism of craniocerebral firearm injury. Methods the German small caliber rifle bullets in the canine model of penetrating craniocerebral injury as the object, using immunohistochemical method to detect brain tissue of control group and different time after injury trajectory contusion area. The expression of C -fos gene and brainstem neurons in the shock region. Results in the control group, cerebral cortical neurons in C -fos gene expression was weak, ballistic contusion area and c-fos shock region and brainstem neurons. Gene expression began to increase at 30min after injury, reached the peak at 2h, 3H and C decreased gradually, the expression of -fos gene in the shock region is ballistic the contusion area is more obvious (P 0.05). Conclusion the brain tissue of dogs ballistic contusion area and the expression of c-fos gene in the shock region and brainstem neurons is the early response to injury stimulation, Its expression may be caused by disseminated inhibition of Leao and is associated with intracellular and extracellular signal transduction and apoptosis.

【作者单位】：第四军医大学附属西京医院全军神经外科研究所第四军医大学附属西京医院全军神经外科研究所成都军区昆明总医院神经外科第四军医大学附属西京医院全军神经外科研究所第四军医大学附属西京医院全军神经外科研究所第四军医大学附属西京医院全军神经外科研究所
【基金】：全军“九五”医学科研基金重点资助项目 ( 99-Z -14 6)
【分类号】：R82

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