ATM介导白藜芦醇改善高脂诱导胰岛素抵抗及骨骼肌糖脂代谢紊乱的研究
发布时间：2021-01-08 19:36
流行病学资料显示,肥胖及2型糖尿病（type 2 diabetes mellitus,T2DM）等代谢性疾病已成为全球公共健康问题,这些代谢性疾病重要的病理基础之一是胰岛素抵抗（insulin resistance,IR）,而骨骼肌IR是全身IR的重要病因之一。近年我国饮食习惯受到西方文化的影响,高脂高热量食物消耗量明显增加。T2DM及肥胖与不良的饮食和作息习惯,尤其是脂肪的大量摄入有关,但其具体机制尚不十分清楚。因此,高脂饮食导致的IR或脂质沉积是较好的研究其机制和寻求治疗方法的模型。我们课题组的前期研究发现高脂能够诱导大鼠及小鼠的IR模型,并伴有骨骼肌脂质沉积。因此,本研究选取了高脂诱导的IR模型作为实验模型。白藜芦醇（resveratrol,Rsv）是一种多酚类植物物质,被称为天然的“热量限制类似物”。近些年Rsv因其抗炎、抗氧化、改善胰岛素敏感性及抗肿瘤等多种功效日益受到人们的关注,但具体机制存在争议,仍有待深入研究。共济失调毛细血管扩张症突变（ataxia-telangiectasia-mutated,ATM）基因是共济失调毛细血管扩张症（ataxia-telangiecta...
【文章来源】：河北医科大学河北省
【文章页数】：115 页
【学位级别】：博士
【部分图文】：
透射电镜观察高脂饮食8周对C57BL6/J小鼠骨骼肌超微结构的影响
研 究 论 文33图7 透射电镜观察白藜芦醇干预8 周对高脂喂养C57BL6/J小鼠骨骼肌超微结构的影响（15kx）Fig.7 Observation by electron microscope of ultrastructure of skeletal musclein HFD C57BL6/J mice after 8-week Rsv intervention（15kx）The three groups are as follows, (A) ND group: normal food diet, (B) HFDgroup: high fat diet, (C) HFD+Rsv group: high fat diet with Rsv 100mg/kg/d,ig. Compared with (B) HFD group, Rsv decreased fat accumulation andameliorated mitochondrial swelling in skeletal muscle as measured by electronmicroscope with magnification 15k. Triangles indicate mitochondria andarrows indicate lipid droplets.A15kxB15k
A 干预对分化后肌细胞胞质内脂滴intervention on lipid droplets in cytoplmuscle cellswas used to observe the cytoplasmicmagnification of photomicrographs wr differentiation, cells were cultured inroup. After differentiation, cells were PA 0.2mmol/L for 24 hours. Lipid dere stained blue.
本文编号：2965176
【文章来源】：河北医科大学河北省
【文章页数】：115 页
【学位级别】：博士
【部分图文】：
透射电镜观察高脂饮食8周对C57BL6/J小鼠骨骼肌超微结构的影响
研 究 论 文33图7 透射电镜观察白藜芦醇干预8 周对高脂喂养C57BL6/J小鼠骨骼肌超微结构的影响（15kx）Fig.7 Observation by electron microscope of ultrastructure of skeletal musclein HFD C57BL6/J mice after 8-week Rsv intervention（15kx）The three groups are as follows, (A) ND group: normal food diet, (B) HFDgroup: high fat diet, (C) HFD+Rsv group: high fat diet with Rsv 100mg/kg/d,ig. Compared with (B) HFD group, Rsv decreased fat accumulation andameliorated mitochondrial swelling in skeletal muscle as measured by electronmicroscope with magnification 15k. Triangles indicate mitochondria andarrows indicate lipid droplets.A15kxB15k
A 干预对分化后肌细胞胞质内脂滴intervention on lipid droplets in cytoplmuscle cellswas used to observe the cytoplasmicmagnification of photomicrographs wr differentiation, cells were cultured inroup. After differentiation, cells were PA 0.2mmol/L for 24 hours. Lipid dere stained blue.
本文编号：2965176
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