Utilization of Cordyceps,Mild Stress and Endurance Training
发布时间：2021-08-11 05:44
目前医学研究人员面临的最迫切问题之一是肥胖人群数量的不断增加以及由肥胖引发的糖尿病和心血管综合征（Syndrome）群体的不断升高。高脂肪和高糖饮食,加上缺少运动的生活方式,是世界上肥胖增多的主要原因。肥胖导致的心血管疾病日益增多,非药物治疗代谢综合征法包括良好的饮食,合理的体重维持,规律的锻炼和科学的膳食补充剂。众所周知,运动训练可降低几种与年龄有关的疾病发病率,包括心血管疾病和Ⅱ型糖尿病。此外,运动训练是一种实用有效,非侵入性的干预措施,它对疾病的防治人类具有重要的作用。传统中药冬虫夏草具有对中枢神经系统有镇静、抗惊厥、降温等作用,有抗肿瘤,抗疲劳,对体液免疫功能有增强作用。此外,冬虫夏草可提高人类心脏耐缺氧能力,降低心脏对氧的消耗,抗心律失常,可以降低血液中的胆固醇和甘油三酯,提高对人体有利的高密度脂蛋白,减轻动脉粥样硬化,然而其中机制还不是很清楚。心脏收缩泵血功能正常与钙离子信号调控密切相关,其中CAMRA、Lamin C、PPTAC、CaMKⅡ和SERCA等钙离子相关蛋白对心脏功能具有保护作用。PI3K/Akt/mTOR信号通路可感受并结合不同的营养因素和环境因素,包括生长因...
【文章来源】：陕西师范大学陕西省 211工程院校 教育部直属院校
【文章页数】：159 页
【学位级别】：博士
【文章目录】：
摘要
Abstract
Chapter Ⅰ Review of the factors that affect cardiac performance and the role of relevant genes
1.1 Conservation of Drosophila and mammalian CAMRs
1.1.1 The role of CAMRs in the nervous system
1.1.2 Involvement of CAMRs in the Notch signaling pathways
1.1.3 Role of CAMRs in cell viability and piRNA pathway
1.1.4 Association between RyRs, TRP/TRPL and CAMRs
1.2 Other relevant cardiac related genes
1.2.1 The role of PPTA gene in cardiac performance
1.2.2 The function of CaMKⅡ gene in cardiac performance
1.2.3 The SERCA gene and cardiac function
1.2.4 The role of Lamin C in cardiomyopathy
1.2.5 Integrin and cardiac function
1.2.6 The effect of Delta in cardiac function
1.3 Effect of Drosophila melanogaster endurance training assays on cardiac performance
1.3.1 Effect of exercise training on cardiovascular disease
1.3.2 Endurance training assays for Drosophila melanogaster
1.4 Fat and sugar metabolism in mammals and Drosophila
1.4.1 Excess fat and sugar in Drosophila causes diabetic cardiomyopathy-like symptoms
1.4.2 Effect of high-dat diet (HFD) on Drosophila heart function
1.4.3 Effect of high-sugar diet (HSD) on Drosophila heart function
1.4.4 Induced-obesity
1.5 Mild stress
1.6 Starvation resistance
1.7 The uses of Cordyceps militaris
1.8 The purpose and significance of the study
Chapter Ⅱ Bioinformatics analysis of calcium and cardiac related proteins
2.1 Introduction
2.2 Materials and methods
2.2.1 Data-mining, protein structure and functional prediction
2.2.2 Analysis of conserved motifs
2.2.3 Construction of the PPI network
2.3 Results
2.4 Discussion
2.5 Conclusion
Chapter Ⅲ Cordyceps improves cardiac performance and aging in endurance training Drosophila melanogaster
3.1 Introduction
3.2 Materials and methods
3.2.1 Fly maintenance and collection
3.2.2 Drug administration
3.2.3 Mortality assay
3.2.4 Lifespan assay for wild type flies
3.2.5 Negative geotaxis assay
3.2.6 Larval crawling assay
3.2.7 Cold stress
3.2.8 RNA extractions and RT-PCR
3.2.9 qRT-PCR
3.2.10 Total protein measurement
3.2.11 Western blot analysis
3.2.12 Courtship and mating
3.2.13 Statistical analysis
3.3 Results
3.3.1 The role of Cordyceps and exercise in aging and cardiac function
3.3.2 Effect of exercise and Cordyceps on Gene and protein expression
3.3.3 Cordyceps does not improve mating and courtship
3.4 Discussion
3.5 Conclusion
Chapter Ⅳ The effect of induced obesity and Cordyceps militaris in Drosophila melanogaster cardiac performance and aging
4.1 Introduction
4.2 Materials and methods
4.2.1 Fly rearing and collection
4.2.2 HFD and HSD feeding regime
4.2.3 Drug administration
4.2.4 Lifespan assay for different fly strains
4.2.5 Negative geotaxis assay for different fly strains
4.2.6 Triglyceride, protein and glucose measurements
4.2.7 Cold stress
4.2.8 RNA extractions and RT-PCR
4.2.9 qRT-PCR
4.2.10 Western blot analysis
4.2.11 Statistical analysis
4.3 Results
4.3.1 The effect of Cordyceps militaris in longevity and cardiac function of mutant flies
4.3.2 Phenotypic profiles of flies fed with high-fat and high-sugar diet
4.3.3 Effect of lipogenic diet on gene and protein expression
4.4 Discussion
4.5 Conclusion
Chapter Ⅴ Effect of combined stresses on Drosophila cardiac performance and mobility
5.1 Introduction
5.2 Materials and methods
5.2.1 Fly rearing and collection
5.2.2 Starvation resistance assay
5.2.3 Negative geotaxis assay
5.2.4 Cold pretreatment
5.2.5 Cold stress
5.2.6 Triglyceride, protein and glucose measurements
5.2.7 RNA extractions and RT-PCR
5.2.8 qRT-PCR
5.2.9 Western blot analysis
5.2.10 Statistical analysis
5.3 Results
5.3.1 The effect of mild stresses in cardiac function of flies
5.3.2 The effect of starvation resistance in the expression of cardiac related genes
5.4 Discussion
5.5 Conclusion
Chapter Ⅵ Summary and Conclusion
References
Appendices
Acknowledgement
Published articles
Scholarship Awards
本文编号：3335574
【文章来源】：陕西师范大学陕西省 211工程院校 教育部直属院校
【文章页数】：159 页
【学位级别】：博士
【文章目录】：
摘要
Abstract
Chapter Ⅰ Review of the factors that affect cardiac performance and the role of relevant genes
1.1 Conservation of Drosophila and mammalian CAMRs
1.1.1 The role of CAMRs in the nervous system
1.1.2 Involvement of CAMRs in the Notch signaling pathways
1.1.3 Role of CAMRs in cell viability and piRNA pathway
1.1.4 Association between RyRs, TRP/TRPL and CAMRs
1.2 Other relevant cardiac related genes
1.2.1 The role of PPTA gene in cardiac performance
1.2.2 The function of CaMKⅡ gene in cardiac performance
1.2.3 The SERCA gene and cardiac function
1.2.4 The role of Lamin C in cardiomyopathy
1.2.5 Integrin and cardiac function
1.2.6 The effect of Delta in cardiac function
1.3 Effect of Drosophila melanogaster endurance training assays on cardiac performance
1.3.1 Effect of exercise training on cardiovascular disease
1.3.2 Endurance training assays for Drosophila melanogaster
1.4 Fat and sugar metabolism in mammals and Drosophila
1.4.1 Excess fat and sugar in Drosophila causes diabetic cardiomyopathy-like symptoms
1.4.2 Effect of high-dat diet (HFD) on Drosophila heart function
1.4.3 Effect of high-sugar diet (HSD) on Drosophila heart function
1.4.4 Induced-obesity
1.5 Mild stress
1.6 Starvation resistance
1.7 The uses of Cordyceps militaris
1.8 The purpose and significance of the study
Chapter Ⅱ Bioinformatics analysis of calcium and cardiac related proteins
2.1 Introduction
2.2 Materials and methods
2.2.1 Data-mining, protein structure and functional prediction
2.2.2 Analysis of conserved motifs
2.2.3 Construction of the PPI network
2.3 Results
2.4 Discussion
2.5 Conclusion
Chapter Ⅲ Cordyceps improves cardiac performance and aging in endurance training Drosophila melanogaster
3.1 Introduction
3.2 Materials and methods
3.2.1 Fly maintenance and collection
3.2.2 Drug administration
3.2.3 Mortality assay
3.2.4 Lifespan assay for wild type flies
3.2.5 Negative geotaxis assay
3.2.6 Larval crawling assay
3.2.7 Cold stress
3.2.8 RNA extractions and RT-PCR
3.2.9 qRT-PCR
3.2.10 Total protein measurement
3.2.11 Western blot analysis
3.2.12 Courtship and mating
3.2.13 Statistical analysis
3.3 Results
3.3.1 The role of Cordyceps and exercise in aging and cardiac function
3.3.2 Effect of exercise and Cordyceps on Gene and protein expression
3.3.3 Cordyceps does not improve mating and courtship
3.4 Discussion
3.5 Conclusion
Chapter Ⅳ The effect of induced obesity and Cordyceps militaris in Drosophila melanogaster cardiac performance and aging
4.1 Introduction
4.2 Materials and methods
4.2.1 Fly rearing and collection
4.2.2 HFD and HSD feeding regime
4.2.3 Drug administration
4.2.4 Lifespan assay for different fly strains
4.2.5 Negative geotaxis assay for different fly strains
4.2.6 Triglyceride, protein and glucose measurements
4.2.7 Cold stress
4.2.8 RNA extractions and RT-PCR
4.2.9 qRT-PCR
4.2.10 Western blot analysis
4.2.11 Statistical analysis
4.3 Results
4.3.1 The effect of Cordyceps militaris in longevity and cardiac function of mutant flies
4.3.2 Phenotypic profiles of flies fed with high-fat and high-sugar diet
4.3.3 Effect of lipogenic diet on gene and protein expression
4.4 Discussion
4.5 Conclusion
Chapter Ⅴ Effect of combined stresses on Drosophila cardiac performance and mobility
5.1 Introduction
5.2 Materials and methods
5.2.1 Fly rearing and collection
5.2.2 Starvation resistance assay
5.2.3 Negative geotaxis assay
5.2.4 Cold pretreatment
5.2.5 Cold stress
5.2.6 Triglyceride, protein and glucose measurements
5.2.7 RNA extractions and RT-PCR
5.2.8 qRT-PCR
5.2.9 Western blot analysis
5.2.10 Statistical analysis
5.3 Results
5.3.1 The effect of mild stresses in cardiac function of flies
5.3.2 The effect of starvation resistance in the expression of cardiac related genes
5.4 Discussion
5.5 Conclusion
Chapter Ⅵ Summary and Conclusion
References
Appendices
Acknowledgement
Published articles
Scholarship Awards
本文编号：3335574
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