蜈蚣提取物对人肝癌细胞HepG2及肝癌原位移植瘤STAT3信号通路的影响（英文）

发布时间：2024-05-13 03:13

　　目的通过观察蜈蚣提取物(CSE)对人肝癌细胞Hep G2及裸鼠肝癌原位移植瘤的影响,探讨其抗肝癌的机制。方法 Hep G2细胞分为CSE₂₅₀(250μg/m L)处理组、CSE₅₀₀(500μg/m L)处理组、5-FU(5-氟尿嘧啶)处理组和对照组,采用酶解丙酮沉淀法分离纯化CSE并作用于人肝癌细胞HepG2,CCK8法检测细胞增殖抑制,流式细胞术检测细胞周期变化。Western blot检测CSE处理后HepG2细胞信号传导与转录激活因子3(STAT3)信号通路相关蛋白表达变化。制备人肝癌细胞裸鼠原位移植瘤模型,随机分为模型组、5-FU组、CSE₁₀组[10mg/(kg·d)]和CSE₅₀组[50mg/(kg·d)],每组12只,观察裸鼠原位移植瘤瘤体体积及质量变化,并检测瘤体p-STAT3、p38丝裂原激活的蛋白激酶(p38MAPK)蛋白表达水平。取模型组及CSE₅₀组裸鼠肝原位移植瘤组织,采用蛋白酪氨酸激酶(PTKs)芯片技术检测并对差异蛋白进行GO及KEGG分析。结...

【文章页数】：13 页

【文章目录】：
1 Introduction
2 Materials and Methods
    2.1 Animals and cell lines
    2.2 Drug preparation
    2.3 In vitro test indicator
        2.3.1 Grouping and cell proliferation inhibition rate measurement
        2.3.2 Cell cycle detection
        2.3.3 Detection of STAT3 pathway protein expression
    2.4 Animal model preparation and group drug administration
        2.4.1 Preparation of nude mice with orthotopic livertumors
        2.4.2 Animal grouping and intervention
    2.5 In vivo test indicators
        2.5.1 Tumor suppression rate
        2.5.2 Protein tyrosine kinase(PTK)chip assay
        2.5.3 Gene Ontology(GO)and Kyoto Encyclopediaof Genes and Genomes(KEGG)enrichment analysis
    2.6 Statistical analysis
3 Results
    3.1 Inhibitory effects of CSE on human liver cancer HepG2 cell proliferation
    3.2 Effects of CSE on HepG2 cell cycle
    3.3 STAT3 pathway protein expression
    3.4 Effects of CSE on the mass and volume of livercancer orthotopic transplantation tumor,as well as the protein level of STAT3 signaling pathway
    3.5 PTK antibody chip screening results
    3.6 Prediction of differential protein’s funtion by GO and KEGG analysis using Metascape
4 Discussion



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