MMP-14和CD31-MVD在结直肠癌组织中的表达及临床意义

发布时间：2018-03-16 01:05

本文选题：结直肠癌　切入点：基质金属蛋白酶-　出处：《安徽医科大学学报》2017年11期 　论文类型：期刊论文

【摘要】：目的探讨基质金属蛋白酶-14(MMP-14)和CD31标记的微血管密度(CD31-MVD)在结肠癌组织中的表达及其与肿瘤的临床意义。方法采用免疫组织化学方法检测60例结直肠癌患者的癌组织及20例正常切缘组织中MMP-14和CD31-MVD的表达。采用qRT-PCR的方法检测20例新鲜结直肠癌组织及正常切缘组织中MMP-14 mRNA的相对表达量。结果 (1)结直肠癌组织中的MMP-14阳性表达率以及CD31-MVD值明显高于正常切缘组织(P0.05);(2)MMP-14的表达及CD31-MVD值与肿瘤大小、浸润深度、TNM分期和淋巴结转移有相关性(P0.05),而与患者性别、年龄无明显相关;(3)MMP-14的表达与CD31-MVD呈正相关性(P0.05);(4)结直肠癌组织中MMP-14 mRNA的相对表达量显著高于正常切缘组织(P0.01)。结论结直肠癌组织中MMP-14的高表达和CD31标记的肿瘤组织微血管密度与结直肠癌的发生、浸润、转移有密切关系。
[Abstract]:Objective to investigate the expression of matrix metalloproteinase-14 (MMP-14) and CD31 labeled microvessel density (CD31-MVD) in colon cancer tissues and its clinical significance. Methods Immunohistochemical method was used to detect the cancer tissues of 60 patients with colorectal cancer. QRT-PCR method was used to detect the relative expression of MMP-14 mRNA in 20 fresh colorectal cancer tissues and 20 normal incisal margin tissues. Results the positive expression rate of MMP-14 was detected in 20 cases of colorectal cancer tissues. The expression of MMP-14, CD31-MVD and tumor size were significantly higher in CD31-MVD than in normal incisal tissue. TNM stage and lymph node metastasis were correlated with the depth of invasion (P 0.05), but with the sex of the patients. There was no significant correlation between the expression of MMP-14 and CD31-MVD. The relative expression of MMP-14 mRNA in colorectal cancer tissues was significantly higher than that in normal incisal margin tissues (P 0.01). Conclusion the high expression of MMP-14 and CD31 labeled microblood in colorectal cancer tissues are significantly higher than those in normal incisal margin tissues. Tube density and the occurrence of colorectal cancer, Infiltration and metastasis are closely related.
【作者单位】：石河子大学医学院;石河子大学医学院第一附属医院胃肠乳腺外科;
【基金】：新疆生产建设兵团卫生科技计划(编号:2013-32)
【分类号】：R735.34

【相似文献】