循环回流发酵与膜分离、泡沫分离耦合工艺生产多粘菌素E

发布时间：2018-04-19 05:42

本文选题：发酵 + 发酵与泡沫分离耦合　；参考：《高校化学工程学报》2017年01期

【摘要】：为了提高多粘菌素E的效价,提出了一种多粘菌素E的生产工艺,应用发酵、膜分离与泡沫分离耦合通过蠕动泵构成循环回流装置生产多粘菌素E。此工艺发酵生产多粘菌素E,可以延长菌体生长的稳定期,有效解除产物抑制,防止菌体被泡沫带出,保证营养物质充分利用。研究了不同耦合开始时间、间歇时间、流速、单次分离时间对多粘菌素E效价的影响。结果表明,设定在23.0 h开始耦合,间歇时间为1.0 h,液体流速为20 m L×min~(-1),单次分离时间为45 min的条件下,发酵48.0 h多粘菌素E的效价达到28634 U×m L~(-1),相比非耦合发酵多粘菌素E的效价(23597U×m L~(-1))提高了21%。总的来说,该工艺有效地提高了多粘菌素E效价,对于其他表面活性物质的分离也有重要的意义。
[Abstract]:In order to improve the titer of polymyxin E, a production process of polymyxin E was put forward. By using fermentation, membrane separation and foam separation, polymyxin E was produced by a circulating reflux device composed of peristaltic pump.The production of polymyxin E by this process can prolong the stable period of cell growth, effectively remove the inhibition of the product, prevent the bacteria from being carried out by foam, and ensure the full utilization of nutrients.The effects of different coupling start time, intermittent time, flow rate and single separation time on the titer of polymyxin E were studied.The results show that when the coupling is started at 23.0 h, the intermittent time is 1.0 h, the liquid flow rate is 20 mL 脳 min-1, the single separation time is 45 min.The titer of polymyxin E reached 28634 U 脳 mL ~ (-1) after 48.0h fermentation, which was 21% higher than that of uncoupled fermentation (23597U 脳 mL ~ (-1)).In general, this process can effectively improve the titer of polymyxin E, and it is also of great significance for the separation of other surface active substances.
【作者单位】：河北工业大学化工学院;
【基金】：河北省高等学校自然科学研究重点项目(ZD2016072)
【分类号】：TQ927;TQ028.8

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