低氧运动对肥胖大鼠体重控制及其骨骼肌线粒体自噬影响的研究

发布时间：2018-01-16 22:16

本文关键词：低氧运动对肥胖大鼠体重控制及其骨骼肌线粒体自噬影响的研究　出处：《曲阜师范大学》2016年硕士论文　论文类型：学位论文

【摘要】：1.研究目的肥胖是由不良生活方式引起,并且严重威胁人类健康,因此,如何控制体重是当今的研究热点、难点。研究发现,低氧和运动均可以起到控制体重的作用,并且能增强骨骼肌线粒体自噬。本课题采用高脂饮食动物肥胖模型,通过观察低氧运动对肥胖大鼠体重控制及骨骼肌线粒体自噬的影响,探讨其机制,为大众健身、减肥提供科学依据。2.试验方法肥胖模型的建立,购进200只3周龄SD大鼠,随机分组前体重无显著性差异,选取20只为普通饲料组,180只为高脂饲料组,饲养12周,当高脂饲料组中出现大鼠体重比普通饲料组平均体重高出20%即判定建模成功,高脂饲料组里选取40只肥胖大鼠,随机分为4组,即常氧安静组、常氧运动组、低氧安静组、低氧运动组,每组10只,共训练4周,末次训练后24h处死,腹腔取血、取腓肠肌肉,测定血脂及相应自噬基因的相对表达量。3.实验结果(1)大鼠体重、Lee’s指数和脂体比的变化与常氧安静组相比,常氧运动组和低氧安静组体重、Lee’s指数无明显变化(p0.05),脂体比有显著性下降(p0.05),低氧运动组体重、Lee’s指数和脂体比均非常显著性下降(p0.01);与常氧运动组相比,低氧运动组体重显著性下降(p0.05),Lee’s指数非常显著性下降(p0.01),脂体无明显变化;与低氧安静组相比,低氧运动组体重、Lee’s指数非常显著性下降(p0.01),脂体比无明显变化。(2)大鼠血液中脂质的变化。与常氧安静组相比,常氧运动组TC、TG和LDL-C均显著性下降(p0.05),HDL-C则明显升高(p0.05),低氧运动组TC、TG与LDL-C均非常显著性下降(p0.01),HDL-C非常显著性升高(p0.01),低氧安静组各指标无显著性变化;与常氧运动组相比,低氧运动组各指标无明显变化;与低氧安静组相比,低氧运动组TC、TG非常显著下降(p0.01),HDL-C和LDL-C则无显著性变化。(3)大鼠自噬相关基因表达的变化与常氧安静组相比,常氧运动组自噬相关基因Beclin-1、P62、LC3表达量升高不明显,低氧安静组和低氧运动组自噬相关基因Beclin-1、P62、LC3表达量均极其显著性升高(p0.001);与常氧运动组相比,低氧运动组自噬相关基因Beclin-1非常显著性升高(p0.01),P62和LC3表达量均极其显著性升高(p0.001);低氧安静组与低氧运动组相比自噬相关基因表达量无明显变化。4.结论(1)低氧与常氧状态下,运动均能促进大鼠血脂代谢,降低动脉粥样硬化和心脑血管疾病的发生风险。(2)低氧运动和常氧运动均有助于减肥,并且低氧运动减肥效果更为明显。(3)低氧运动能显著提升自噬基因的相对表达量,促进自噬的发生,对维持机体细胞内环境的稳态有重要作用。
[Abstract]:1. Objective Obesity is caused by unhealthy lifestyle and is a serious threat to human health. Therefore, how to control weight is a hot and difficult point. Hypoxia and exercise can both control body weight and enhance mitochondrial autophagy of skeletal muscle. By observing the effect of hypoxic exercise on weight control and mitochondrial autophagy of skeletal muscle in obese rats, the mechanism of hypoxia exercise was discussed, which provided a scientific basis for mass fitness and weight loss. Two hundred 3-week-old SD rats were purchased. There was no significant difference in body weight before randomized grouping. 20 rats were selected as normal diet group and 180 rats were fed high-fat diet group for 12 weeks. When the body weight of the rats in the high-fat diet group was 20% higher than the average body weight of the normal diet group, 40 obese rats in the high-fat diet group were selected and randomly divided into 4 groups, that is, the normoxic quiet group. Normoxic exercise group, hypoxic quiet group and hypoxic exercise group, 10 rats in each group, were trained for 4 weeks, killed 24 hours after the last training, blood was taken from abdominal cavity and gastrocnemius muscle was taken. The relative expression of serum lipids and the corresponding autophagy gene was measured. The results were as follows: 1) the changes of the body weight index and the ratio of lipid to body weight of rats were compared with those of normoxic exercise group and hypoxic quiet group, compared with that of normoxic exercise group and hypoxic quiet group. There was no significant change in Lee's index (P 0.05), and the ratio of lipid to body decreased significantly (P 0.05). The body weight of hypoxic exercise group was lower than that of control group. The Lee's index and the ratio of lipid to body decreased significantly (P 0.01). Compared with the normoxic exercise group, the body weight of hypoxic exercise group was significantly lower than that of normoxic exercise group. Compared with hypoxic quiet group, the body weight and Leehs index of hypoxic exercise group decreased significantly (P 0.01). Compared with normoxic quiet group, TC TG and LDL-C in normoxic exercise group decreased significantly (p 0.05). The levels of TG and LDL-C in hypoxic exercise group were significantly lower than those in control group (P 0.01). HDL-C was significantly increased in hypoxic exercise group (P 0.01). There was no significant change in the indexes of hypoxic quiet group. Compared with normoxic exercise group, the indexes of hypoxic exercise group did not change significantly. Compared with the hypoxic quiet group, the TG of hypoxic exercise group decreased significantly (p0.01). HDL-C and LDL-C showed no significant changes.) the expression of autophagy related genes in normal oxygen exercise group was higher than that in normoxic quiet group, and Beclin-1 gene P62 in normoxic exercise group. The expression of LC3 was not significantly increased in hypoxic quiescent group and hypoxic exercise group, and the expression of Beclin-1 p62mLC3 gene was significantly increased in hypoxic quiescent group and hypoxic exercise group. Compared with the normoxic exercise group, the expression of autophagy related gene Beclin-1 in hypoxic exercise group was significantly higher than that in the normoxic exercise group. The expression of p0.01p62 and LC3 in hypoxia exercise group was significantly higher than that in normoxic exercise group. There was no significant change in the expression of autophagy related genes in hypoxic quiet group and hypoxic exercise group compared with hypoxic exercise group. Conclusion: conclusion: 1) under hypoxia and normoxic condition, exercise can promote the metabolism of blood lipids in rats. Reduce the risk of atherosclerosis and cardiovascular and cerebrovascular diseases. 2) hypoxic exercise and normoxic exercise are conducive to weight loss. Hypoxia exercise can significantly increase the relative expression of autophagy genes, promote autophagy, and play an important role in maintaining the homeostasis of intracellular environment.
【学位授予单位】：曲阜师范大学
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：G804.2

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