补充β-丙氨酸对间歇训练大鼠糖代谢指标的影响

发布时间：2018-04-01 22:18

本文选题：β-丙氨酸　切入点：肌肽　出处：《北京体育大学》2015年硕士论文

【摘要】：目的：通过给间歇运动训练的大鼠补充p-丙氨酸,观察大鼠骨骼肌中肌肽含量的变化,探索补充p-丙氨酸对间歇训练大鼠骨骼肌以及肝脏糖代谢的影响。方法：将40只八周龄的健康雄性SD大鼠,随机分成4组(N=10)：间歇运动中等剂量补剂组(A组)、间歇运动高剂量补剂组(B组)、间歇运动补水组(C组)、空白对照组(D组)。运动方式采用高速间歇训练,跑台速度为50 m/min,时间6 min,休息5 min后,继续依此方式共训练3次,每周6天,共4周。补剂采用灌胃方式,中等剂量组补充剂量为1g/kg体重,高剂量组补充剂量为1.5g/kg体重,补水组和空白对照组则为相同体积的蒸馏水。然后采用邻苯二甲醛柱前衍生化法测试腓肠肌中肌肽含量、比色法测腓肠肌肌糖原含量、比色法测腓肠肌己糖激酶活性；葡萄糖氧化酶法测血清中葡萄糖含量；比色法测肝脏中肝糖原含量以及实时荧光定量PCR测肝脏葡萄糖-6-磷酸酶、糖皮质激素受体、糖原合成酶、葡萄糖激酶和胰岛素受体的mRNA相对表达。结果：(1)A组大鼠腓肠肌中肌肽含量显著高于C组(p0.05),B组也显著高于C组(p0.01)；C组大鼠腓肠肌己糖激酶活性显著低于D组,A组显著高于B、C两组(p0.01)。(2)C组的大鼠肝糖原含量显著高于D组(p0.01),A组显著低于B、C两组(p0.05)；C组大鼠肝脏中葡糖糖-6-磷酸酶的mRNA相对表达显著高于D组(p0.01)；A组大鼠肝脏中糖皮质激素受体的mRNA相对表达显著高于B、C两组(p0.01)；C组大鼠肝脏中糖原合成酶的mRNA相对表达显著低于D组(p0.01)；A组大鼠胰岛素受体的mRNA相对表达显著高于B组(p0.01)。结论：补充p-丙氨酸可以促进间歇训练大鼠肌肉对血糖的利用,增加肝脏糖原的分解,中等剂量效果更好。
[Abstract]:Objective: to observe the change of carnosine content in skeletal muscle of rats by adding palanine to intermittent exercise training rats. To explore the effect of supplementation of p-alanine on glucose metabolism in skeletal muscle and liver of rats after intermittent training. The rats were randomly divided into 4 groups: group A with moderate dose of intermittent exercise, group B with high dose of intermittent exercise, group C with intermittent exercise, group D with blank control group. The treadmill speed was 50 m / min, time 6 minutes, rest for 5 min, then continued to train 3 times a week, 6 days a week, for 4 weeks. The supplementation was done by gavage, the medium dose group was 1g/kg body weight, and the high dose group was 1.5g/kg body weight. The contents of carnosine in gastrocnemius muscle, glycogen content in gastrocnemius muscle and hexokinase activity in gastrocnemius muscle were measured by pre-column derivatization method, colorimetric method and colorimetric method respectively. Glucose content in serum was measured by glucose oxidase method, liver glycogen content was measured by colorimetric method, glucose-6-phosphatase, glucocorticoid receptor and glycogen synthase were measured by real-time fluorescence quantitative PCR. Results the content of carnosine in gastrocnemius in group A was significantly higher than that in group C (p0.05) and group B was significantly higher than that in group C (P 0.01). The activity of hexokinase in gastrocnemius was significantly lower in group A than in group D. The liver glycogen content of rats in group C was significantly higher than that in group D (P 0.01). The mRNA expression of glucose-6-phosphatase in liver of rats in group B and C was significantly higher than that in group D (p0.01A) and the expression of glucocorticoid receptor (Glucocorticoid receptor) in liver of group D (p0.01A). The relative expression of glycogen synthase (mRNA) in liver of group C was significantly higher than that of group B (P 0.01). Conclusion: the relative expression of mRNA of insulin receptor in group A is significantly higher than that in group B (P 0.01). Conclusion: supplement of palanine can increase the expression of glycogen synthase in liver of rats. Promoting intermittent training of the use of blood glucose in rat muscles, Increased liver glycogen decomposition, medium dose effect is better.
【学位授予单位】：北京体育大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：G804.7

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