持续与间歇跑台运动对大鼠空间学习记忆及海马、皮质氧化抗氧化能力的机制研究

发布时间：2018-05-04 05:39

本文选题：持续运动 + 间歇运动　；参考：《云南师范大学》2017年硕士论文

【摘要】：研究目的:运动科学领域越来越多的研究表明,适宜的运动对学习记忆能力具有促进作用。本研究通过6周有氧跑台训练对SD大鼠学习记忆能力、海马和皮质氧化抗氧化的影响,探讨持续与间歇运动促进大鼠学习记忆的提高,其作用机制可能是通过改变MDA、SOD代谢及NO的表达来实现。研究方法:实验动物进行适应性饲养后进行筛选,筛选方法采用水迷宫可视平台实验,淘汰疑似先天痴呆的大鼠。选取健康雄性成年SD大鼠24只,随机分为空白对照组(n=8)、持续训练组(n=8)和间歇训练组(n=8)。在正式实验前进行1周的适应性跑台训练。跑台正式训练采用递增负荷形式,起始速度为22m/min,坡度为0,每周递增1m/min,进行6周,最后一周速度为27m/min;持续组运动40min,间歇组每次运动20min,每天2次,两次运动间休息3小时。跑台训练结束后三组大鼠进行为期5天的Morris水迷宫实验(4天定位航行实验,1天空间探索实验)。在水迷宫测试后禁食禁水24h后,用3.6%水合氯醛腹腔注射麻醉,取海马、皮质。使用试剂盒测试大鼠海马、皮质MDA含量、SOD活力、NO含量。研究结果:(1)Morris水迷宫检测结果:定位航行实验结果显示,纵向比较发现,定位航行实验中,随训练天数的增加,各组潜伏期减小,站台象限路程%和时间%增加。横向比较发现,第2天,运动组潜伏期与对照组有显著性差异(p0.05);第3天,与对照组相比,持续组(p0.05)与间歇组(p0.01)下降明显,其中间歇组与持续组相比,间歇组明显下降(p0.05);第4天,运动组潜伏期显著低于对照组(p0.01)。站台象限路程%在4天的测试中都是间歇组最高;第3天间歇组显著高于对照组和持续组(p0.05),第4天间歇组明显高于对照组(p0.05)。在4天的测试中,站台象限时间%间歇组最高,持续组次之,对照组最低;第3天间歇组站台象限时间%高于对照组,差异极具显著性(p0.01);第4天间歇组明显高于持续组和对照组,差异极具显著性(p0.01)。空间探索实验结果显示,与对照组相比,持续组与间歇组穿台次数显著增加(p0.01),间歇组多于持续组,但无差异;持续组与间歇组到达潜伏期显著低于对照组(p0.01),间歇组低于持续组,运动组间无差异;站台象限路程%与站台象限时间%间歇组显著高于对照组(p0.05),两运动组间无差异;运动组总路程显著高于对照组(p0.01),运动组间无差异;平均速度持续组(p0.05)与间歇组(p0.01)都显著高于对照组,但运动组间无差异。(2)海马氧化抗氧化能力结果:与对照组相比,持续组和间歇组MDA含量显著降低(P0.01),间歇组低于持续组,但无差异;持续组与间歇组SOD活性显著高于对照组(P0.01),间歇组高于持续组,但组间无差异。与对照组比较,运动组NO含量较高,其中,间歇组显著高于对照组(P0.05),但持续组和间歇组无差异。(3)皮质氧化抗氧化能力结果:持续组、间歇组MDA含量低于对照组(P0.05),间歇组低于持续组,但组间无差异;持续组与间歇组SOD活性高于对照组,间歇组高于持续组,但组间无差异;NO检测结果显示,间歇组最高,持续组次之,对照组最低,三组大鼠之间无差异(P0.05)。研究结论:六周有氧跑台运动改善了大鼠的空间学习记忆能力,使大鼠大脑海马和皮质的SOD、NO活性增高,MDA含量下降,减弱了脂质过氧化对海马和皮质的损伤,间歇运动与持续运动相比,间歇运动对增强学习记忆的效果以及清除自由基的能力强于持续运动。六周跑台运动促进学习记忆的提高可能与运动提高海马、皮质对自由基损伤的抵抗能力,使机体对自由基产生耐受力和适应力,从而提高抗氧化能力以及提高NO的表达有关。
[Abstract]:Research purposes: more and more studies in the field of sports science have shown that appropriate exercise has a promoting effect on learning and memory ability. In this study, the effects of 6 week aerobic table training on the learning and memory ability of SD rats, the oxidation and oxidation resistance of hippocampus and cortex, and the improvement of learning and memory in rats by continuous and intermittent movement were discussed. It may be realized by changing the expression of MDA, SOD metabolism and NO. Research methods: the experimental animals were screened by adaptive feeding, and the screening methods were selected by the water maze visual platform experiment, and the rats with suspected congenital dementia were eliminated. 24 healthy adult male SD rats were selected and randomly divided into blank control group (n=8), continuous training group (n=8) and room. The training group (n=8) was trained for 1 weeks prior to the formal experiment. The formal training used the incremental load form, the starting speed was 22m/min, the gradient was 0, the weekly increase of 1m/min, the 6 week, the last week was 27m/min, the continuous group movement 40min, the interval group each exercise 20min, the two times between the 3 hours between the two exercises. After the end of the training, the three groups of rats were conducted a 5 day Morris water maze test (4 days positioning navigation experiment, 1 day space exploration experiment). After the water maze test was forbidden to water 24h, the hippocampus and cortex were taken with 3.6% chloral chloral injection, the hippocampus and the cortex were taken to test the rat sea horse, the cortical MDA content, the SOD activity, and the NO content. The results were (1) M The results of orris water maze test: the results of the navigation experiment showed that the longitudinal comparison found that the incubation period decreased with the increase of the number of training days in the navigation experiment, and the horizontal comparison found that the incubation period of the sports group was significantly different from the control group (P0.05) on the second day, and the third day, compared with the control group. Group (P0.05) and intermittent group (P0.01) decreased significantly, among which intermittent group was significantly lower than that in continuous group (P0.05); on the fourth day, the latency of the exercise group was significantly lower than that of the control group (P0.01). The platform quadrant route% was the highest in the intermittent group in the 4 day test; the third day interval group was significantly higher than the control group and the continuous group (P0.05), and the fourth day intermittent group was obvious. Higher than the control group (P0.05). In the 4 day test, the platform quadrant time interval group was the highest, the continuous group, the control group was the lowest, the third day interval group platform quadrant time% was higher than the control group, the difference was significant (P0.01); the fourth day interval group was significantly higher than the continuous group and the group, the difference was very significant (P0.01). Spatial exploration experimental results showed that the results showed that Compared with the control group, the number of times in the continuous group and the intermittent group increased significantly (P0.01), and the intermittent group was more than the continuous group, but there was no difference. The duration of the interval group and the intermittent group was significantly lower than that of the control group (P0.01). The intermittent group was lower than the continuous group, and there was no difference between the group and the exercise group; the station platform quadrant% and the platform quadrant time interval group were significantly higher than the control group (p0.0 5) there was no difference between the two exercise groups, the total distance of the exercise group was significantly higher than that of the control group (P0.01), and there was no difference between the exercise groups, and the average speed continuous group (P0.05) and the intermittent group (P0.01) were significantly higher than the control group, but there was no difference between the exercise groups. (2) the oxidation antioxidant capacity of the hippocampus: compared with the control group, the MDA content in the continuous group and the intermittent group was significantly lower (P0.01). The interval group was lower than the continuous group, but no difference was found in the continuous group and the intermittent group SOD activity was significantly higher than the control group (P0.01), the intermittent group was higher than the continuous group, but there was no difference between the group and the control group. Compared with the control group, the NO content of the exercise group was higher than that of the control group (P0.05), but there was no difference between the continuous group and the intermittent group. (3) the oxidation antioxidant capacity of the cortex was found. Fruit: in the continuous group, the MDA content in the intermittent group was lower than the control group (P0.05), the intermittent group was lower than the continuous group, but there was no difference between the group and the group. The SOD activity in the continuous group and the intermittent group was higher than the control group. The intermittent group was higher than the continuous group, but there was no difference between the groups. The results of NO test showed that the interval group was the highest, the continued group was the lowest, and the three groups of rats had no difference (P0.05). Conclusion: the six week aerobic table exercise improved the spatial learning and memory ability of rats, increased the activity of SOD and NO in the hippocampus and cortex of the rat brain, decreased the content of MDA, and weakened the damage to the hippocampus and cortex by lipid peroxidation. Intermittent movement could enhance the effect of learning and memory and scavenge free radicals, compared with the continuous movement. The improvement of learning and memory in the six week treadmill exercise may improve the resistance of the hippocampus, the cortex to the free radical damage, the tolerance and adaptability of the body to the free radicals, so as to improve the antioxidant capacity and to improve the expression of NO.

【学位授予单位】：云南师范大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：G804.2

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