4周低氧运动对营养肥胖型大鼠骨骼肌自噬相关因子时序性变化影响的研究

发布时间：2018-05-18 19:06

本文选题：低氧运动 + 骨骼肌　；参考：《曲阜师范大学》2017年硕士论文

【摘要】：1目的:探讨在低氧条件下运动对营养肥胖型大鼠腓肠肌自噬相关因子P62、LC3、Beclin-1表达的影响以及低氧运动诱导骨骼肌细胞自噬发生的可能机制。2方法:采用高脂饮食诱导SD大鼠建立营养肥胖型大鼠模型,随后选取建模成功大鼠随机分为:对照组(C)、低氧训练1周组(E1)、低氧训练2周组(E2)、低氧训练3周组(E3)和低氧训练4周组(E4),共5组,每组10只。低氧暴露模拟13.6%氧浓度(相当于3500m海拔氧含量),动物跑台进行训练,速度为20m.min-1,坡度为0,每天运动1h,周末休息2天,分别训练1周、2周、3周、4周,最后一次训练结束24h后将动物处死并采样,采用RT-PCR技术检测模型大鼠腓肠肌P62、LC3、Beclin-1 mRNA以及westent blot技术检测P62、LC3、Beclin-1蛋白在实验干预前后的变化情况。3结果:E1、E2、E3组与C组相比Beclin-1 mRNA表达量显著升高(P0.05),E4组略有下降,Beclin-1蛋白表达量在前两周逐渐下降,与C组相比E2组下降程度显著(P0.05),后两周又逐渐增加,E4组比E2组明显升高(P0.01);E1组P62 mRNA的相对表达量与C对照组相比增加明显(P0.05),E2组与E1组相比呈持续升高趋势(P0.05),E3组P62 mRNA的相对表达量非常显著低于E2组和E4组(P0.01)。E3组肥胖大鼠P62蛋白相对表达量与E2组相比显著升高;E2、E3组、E4组LC3 mRNA相对表达量均显著高于C组(P0.05),5组肥胖大鼠LC3蛋白中LC3II/LC3I比值变化均不明显。4结论:(1)低氧运动能显著增加自噬相关基因的表达,在一定程度上促进自噬的发生,但是自噬基因的转录与自噬蛋白质的合成具有不同步性。(2)在低氧训练的前两周骨骼肌的自噬程度出现增加的趋势,但是随低氧运动时间的延长骨骼肌的自噬程度逐渐降低,可能是机体对低氧刺激逐渐适应的结果。
[Abstract]:1 Objective: To investigate the effect of exercise on the expression of autophagy related factors P62, LC3, Beclin-1 in the gastrocnemius and the possible mechanism of autophagy induced by hypoxia in hypoxic rats, as well as the possible mechanism of hypoxic exercise induced autophagy in skeletal muscle cells: a hyperlipidemic diet induced SD rats to establish a obese rat model, and then a rat model was selected and the rats were selected randomly. The control group (C), hypoxia training 1 weeks group (E1), hypoxia training group (E2), hypoxia training 3 weeks group (E3) and hypoxic training 4 weeks group (E4), a total of 5 groups, 10 rats in each group. The hypoxia exposure simulated 13.6% oxygen concentration (equivalent to 3500m altitude oxygen content), the animals were trained on the platform, the speed was 20m.min-1, the slope was 0, every day Sports 1H, weekend for 2 days rest for 2 days, respectively training. After 1 weeks, 2 weeks, 3 weeks and 4 weeks, the animals were killed and sampled after the last training 24h. RT-PCR technique was used to detect the gastrocnemius muscle P62, LC3, Beclin-1 mRNA and westent blot technology to detect the P62, LC3, Beclin-1 protein before and after the experimental intervention. (P0.05), the E4 group decreased slightly and the expression of Beclin-1 protein decreased gradually in the first two weeks. Compared with the C group, the decrease in E2 group was significantly higher than that in the E2 group (P0.05), and in the latter two weeks, the E4 group increased significantly (P0.01), and the relative expression of P62 mRNA in the E1 group was significantly higher than that in the control group. The relative expression of P62 mRNA was significantly lower than that in group E2 and E4 group (P0.01).E3 group, and the relative expression of P62 protein in obese rats was significantly higher than that of E2 group; E2, E3 and E4 groups were significantly higher than those in the group of E2. The variation in the ratio of the 5 groups of obese rats was not obvious: (1) the increase of hypoxic activity was significantly increased The expression of autophagy related genes promoted autophagy to some extent, but the transcription of autophagy gene was not synchrony with the synthesis of autophagic proteins. (2) the degree of autophagy in skeletal muscles increased in the first two weeks of hypoxia training, but the degree of autophagy gradually decreased with the prolonged hypoxia exercise time. The result of the body's gradual adaptation to hypoxic stimulation.
【学位授予单位】：曲阜师范大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：G804.2

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