有氧运动对肥胖小鼠棕色脂肪部分糖脂代谢相关基因表达的影响

发布时间：2018-05-23 09:21

本文选题：棕色脂肪 + 糖脂代谢　；参考：《北京体育大学》2017年硕士论文

【摘要】：目的:本研究通过4周中低强度跑台运动干预肥胖小鼠,RT-q PCR验证基因芯片所选的棕色脂肪组织(BAT)中糖脂代谢相关差异基因mRNA水平,以期从基因转录层面探究有氧运动影响肥胖小鼠BAT糖脂代谢的机制。方法:30只雄性离乳C57BL/6J小鼠随机分为:普通膳食对照组(N)和高脂膳食组(HD)。8周肥胖模型建立后,HD被分为肥胖对照组(OB)和肥胖运动组(E)。运动方案为10m/min,1 h/次/天,6次/周,共4周的跑台训练。干预结束,测试血清甘油三酯(TG)、总胆固醇(CHO)和血糖(GLU)含量;取肩胛处BAT,进行mRNA表达谱芯片杂交扫描分析。筛选差异倍数达2倍以上的差异表达基因,对差异基因进行聚类分析、功能注释(GO)和通路分析(pathway)。根据差异基因的生物功能、筛选出参与糖脂代谢的关键差异基因进行RT-PCR验证。结果:(1)相比于N组,OB组发生上调的基因有445个,下调有796个,差异表达基因的生物功能主要集中为脂质、固醇,胆固醇以及类固醇等的合成代谢过程和白细胞趋药性过程;相比于OB组,E组发生上调的差异基因有486个,下调有286个,差异表达基因的生物功能集中在脂质,脂肪酸和胆固醇的合成代谢过程、羧酸,酮酸和辅酶的代谢过程以及抗氧化过程;(2)运动调控棕色脂肪功能的主要通路集中在AMPK信号通路,PPAR信号通路,Insulin信号通路以及VEGF信号通路等;(3)根据差异基因的差异倍数、生物功能和所在通路,筛选Acsl5、Fasn、Acaca、Fabp5、Fbp1、Ptges和Ptgis为关键差异表达基因;(4)RT-PCR结果显示,OB组BAT中Acsl5、Fasn、Acaca、Fabp5、Fbp1、Ptges和Ptgis mRNA水平较N组有极显著降低;E组Acsl5、Fasn、Acaca、Fabp5和Ptgis mRNA水平较OB组显著升高,证明了芯片结果的准确性。结论:4周有氧运动可通过PPAR、AMPK、Insulin和VEGF信号通路来调节肥胖机体BAT的糖脂代谢相关基因Acsl5、Fasn、Acaca、Fabp5、Fbp1、Ptges和Ptgis的表达而提高BAT活性,降低肥胖小鼠体重和血糖血脂水平,促进机体的健康。
[Abstract]:Objective: to investigate the effects of moderate and low intensity treadmill exercise on the mRNA levels of glucose and lipid metabolism related genes in brown adipose tissue (BATs) selected by RT Q PCR in obese mice after 4 weeks of low intensity treadmill exercise. The aim of this study was to explore the mechanism of aerobic exercise on BAT glucose and lipid metabolism in obese mice at the level of gene transcription. Methods 30 male isolated C57BL/6J mice were randomly divided into two groups: normal diet control group (n) and high fat diet group (HD8 weeks). After the establishment of obesity model, HD was divided into obese control group (OB) and obese exercise group. The exercise program was 10 m / min 1 h / d / day / day 6 times / week for 4 weeks of treadmill training. At the end of the intervention, serum triglyceride (TTG), total cholesterol (Cho) and blood glucose (GLU) were measured, and the scapular BATs were taken for mRNA expression microarray hybridization scanning analysis. The differentially expressed genes were screened and analyzed by cluster analysis, functional annotation (GOG) and pathway analysis. According to the biological function of differentially expressed genes, the key genes involved in glycolipid metabolism were screened and verified by RT-PCR. Results compared with N group, there were 445 up-regulated genes and 796 down-regulated genes. The biological functions of differentially expressed genes were mainly composed of lipid, steroids, cholesterol, steroids and leukocyte chemotaxis. Compared with OB group, there were 486 differentially expressed genes and 286 down-regulated genes. The biological functions of differentially expressed genes were concentrated in lipid, fatty acid and cholesterol synthesis, carboxylic acid, carboxylic acid, The metabolic process of ketoacid and coenzyme and the antioxidant activity of PPAR-2) the main pathways regulating the function of brown fat are concentrated in the AMPK signaling pathway, the AMPK signal pathway, the insulin signal pathway and the VEGF signal pathway, etc.) according to the difference multiple of the different genes, the main pathway of motor regulation of brown fat function is ketoacid and coenzyme. Biological function and pathway, selection of the key differentially expressed genes of Acsl5Fbp1Ptges and Ptgis as the key differentially expressed genes, RT-PCR results showed that the levels of Acsl5FasnFbp1Ptges and Ptgis mRNA in the BAT of the OB group were significantly lower than those of the N group, which proved the accuracy of the microarray results. Conclusion aerobic exercise for 4 weeks can increase the activity of BAT, decrease the body weight and blood glucose and blood lipid, and promote the health of obese mice by regulating the expression of BAT related genes of glucose and lipid metabolism, such as Acsl5Fbp1Ptges and Ptgis, through the signal pathway of PPAR AMPK insulin and VEGF.
【学位授予单位】：北京体育大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：G804.7

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