16周HIIT训练对大鼠骨骼肌MG29蛋白及最大摄氧量的影响

发布时间：2018-06-05 01:58

本文选题：高强度间歇训练 + MG29蛋白　；参考：《北京体育大学》2017年硕士论文

【摘要】：研究目的:通过对29-45周龄大鼠16周HIIT干预,观察影响骨骼肌收缩机能指标MG29蛋白、形态指标体重、有氧能力指标VO2max及整体运动能力指标力竭运动时间的变化特点和规律,探讨HIIT对骨骼肌机能及心肺耐力影响效果,为HIIT应用于预防肌肉衰减综合症、改善心肺耐力提供理论依据。研究方法:56只雄性Wistar大鼠,随机分为基础组(B组,n=6),增龄对照组(C组,n=24)和HIIT运动组(H组,n=26),适应性喂养一周后测试VO2max,之后每两周测试一次并制定相应HIIT运动方案(90%VO2max和50%VO2max高低强度间歇,5d/w,50min/d)。C组不运动,H组执行HIIT。分别在1周、8周、16周时进行力递增负荷竭运动测试和取材。生化测试指标与方法为:股直肌MG29mRNA含量(RT-PCR)、MG29表达(Western-blot)。采用单因素方差分析对测得结果进行数理统计。研究结果:1、与C组比,16周后H组MG29 mRNA含量显著增加(P0.05),而H组MG29蛋白在8周、16周后均显著增加(P0.05);2、与B组比,C组VO2max下降了42.8%(P0.01),体重上升了16%(P0.01),而H组VO2max仅下降了25.9%(P0.01),体重无明显变化;与C组比,H组VO2max在干预9周和15周后显著提高(P0.05),从第4周开始,体重均显著低于C组(P0.05)。3、与C组相比,H组8周和16周力竭时间均显著长于C组(P0.05)。研究结论:1、增龄会导致大鼠体重上升、心肺耐力下降,HIIT训练4周即可显著抑制大鼠增龄性体重上升趋势,至少HIIT训练8周以上才能显著改善心肺耐力。2、29-45周龄年龄增长不会导致大鼠骨骼肌中MG29蛋白表达下降,HIIT训练8周和16周后可显著提高骨骼肌中MG29蛋白表达水平。3、HIIT训练8周和16周均可有效延长大鼠力竭运动时间。
[Abstract]:Objective: to observe the change characteristics and regularity of MG29 protein, body weight, aerobic index VO2max and exhaustive exercise time of skeletal muscle contraction function index (MG29) in rats aged 29-45 weeks after 16 weeks of HIIT intervention. To investigate the effect of HIIT on skeletal muscle function and cardiopulmonary endurance, and to provide theoretical basis for the application of HIIT in the prevention of muscle attenuation syndrome and the improvement of cardiopulmonary endurance. Methods 56 male Wistar rats were studied. The rats were randomly divided into two groups: group B (group B), group C (group C) and group C (HIIT exercise group). VO2maxwas tested after a week of adaptive feeding, and the corresponding regimen of HIIT exercise was determined every two weeks. The interval of 5 days / w50 min / d / d in group C with high and low 50%VO2max intensity was determined. At 1 week 8 weeks and 16 weeks, the exhaustive exercise test and material selection were carried out. The biochemical indexes and methods were as follows: the MG29mRNA content of rectus femoris muscle and the expression of MG29 in RT-PCRG _ (29) were Western-blotte. The single factor analysis of variance was used to carry out mathematical statistics on the measured results. The results showed that MG29 mRNA content in H group was significantly higher than that in C group after 16 weeks, while MG29 protein in H group was significantly higher than that in C group after 8 weeks and 16 weeks. Compared with group B, VO2max in group C was decreased by 42.8% P 0.01, weight increased by 16%, but VO2max in group H decreased only 25.9% (P 0.01), and there was no significant change in body weight. Compared with group C, VO2max in group H increased significantly after 9 and 15 weeks of intervention. From the 4th week, the body weight of group H was significantly lower than that of group C (P 0.05). Compared with group C, the exhaustion time of group H at 8 and 16 weeks was significantly longer than that of group C. The results showed that weight gain in rats was induced by increasing age at 1: 1, and decreased cardiopulmonary endurance and HIIT training for 4 weeks could significantly inhibit the increasing trend of aging weight in rats. At least 8 weeks of HIIT training can significantly improve cardiopulmonary endurance. The increase of age from 29 to 45 weeks will not result in the decrease of MG29 protein expression in skeletal muscle of rats. After 8 weeks and 16 weeks of training, the expression level of MG29 protein in skeletal muscle can be significantly improved. 3HIIT training can significantly improve the expression level of MG29 protein in skeletal muscle. Training for 8 weeks and 16 weeks could effectively prolong the time of exhaustive exercise in rats.
【学位授予单位】：北京体育大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：G804.2

【相似文献】