亚精胺对高温中强度跑台运动小鼠抗氧化能力作用的研究

发布时间：2018-08-01 10:59

【摘要】：目的:探索亚精胺(Spermidine,SPD)对长期和一次性高温中强度跑台运动小鼠抗氧化能力作用的影响。方法:将72只无显著差异的雄性昆明小鼠随机分为俩大组,一次性组和长期性组,每大组随机分为六小组,共12小组,俩大组中各六小组组别一致,分别为一次性和长期性常温中强度跑台运动生理盐水组(ONIS、LNIS)、高温中强度跑台运动Vc组(OHIVc、LHIVc)、高温中强度跑台运动亚精胺低剂量组(OLSPD、LLSPD)、高温中强度跑台运动亚精胺中剂量组(OTSPD、LTSPD)、高温中强度跑台运动高剂量组(OHSPD、LHSPD),高温中强度跑台运动生理盐水组(OHIS、LHIS)、,每组各6只,长期性组训练8周,5d/w,一次性组实验一天,实验期间每天上午10点灌胃,给药标准为:高温中强度跑台运动亚精胺低、中、高剂量组(0.5mmol/kg、1mmol/kg、1.5mmol/kg);高温和常温中强度跑台运动生理盐水组(0.86%)以及阳性对照组高温中强度跑台运动Vc组(1.2g/L)。下午俩点进行中强度跑台训练,中强度按12m/min的速度进行训练,每天训练10min,第6天开始,跑台速度隔日增加3m/min,增加至18m/min停止,持续时间隔日增加10min,增加至20min停止。常温和高温室温分别为23℃、39℃,相对湿度为50%,跑台坡度为0°,无负重。结果:1)经一次性和持续8周高温中强度跑台训练发现:不同剂量SPD对高温运动小鼠各抗氧化指标影响不同。2)持续8周的长期高温跑台研究结果显示:经LHIS较LNIS相比得知,温度升高,长期跑台运动后昆明小鼠血清、心肌和肝脏组织内MDA、H2O2含量均显著提高(P0.05),GSH-Px和T-SOD活性及T-AOC显著降低(P0.05),其中小鼠血清和心肌中T-SOD活性极显著降低(P0.01),NO含量无显著变化(P0.05);LLSPD相较LHIS,血清和心肌内MDA和H2O2的含量极显著降低(P0.01),血清和心肌内GSH-Px活性极显著升高(P0.01),心肌内NO含量显著升高(P0.05),血清、心肌和腓肠肌内T-AOC显著升高(P0.05),肝脏内MDA和显著降低(P0.05),肝脏内GSH-Px、NO、T-AOC无显著变化(P0.05);LLSPD与LHIVc相比,无显著差异(P0.05),心肌效果突出。3)一次性高温中强度跑台训练显示:经OHIS较ONIS相比发现,温度升高,一次性中强度跑台后,小鼠血清、心肌、肝脏和腓肠肌内MDA和H2O2含量显著上升(P0.05),GSH-Px和T-SOD活性及机体T-AOC显著降低(P0.05),血清、心肌和腓肠肌内NO含量显著降低(P0.05),肝脏内NO含量无显著变化(P0.05);OTSPD相较OHIS,心肌内MDA含量显著降低(P0.05),OTSPD与OHIVc相比,无显著差异(P0.05),其它组间无显著差异(P0.05)。结论:1)温度升高,昆明小鼠经中强度跑台后,小鼠血清、肝脏、心肌和腓肠肌内的MDA、H2O2含量升高,NO含量、GSH-PX、T-SOD活性及T-AOC降低,高温可以使机体氧化代谢水平升高,加快自由基生成,加速机体疲劳速率。2)昆明小鼠经长期服用低剂量SPD后可显著提高高温环境下长期跑台抗氧化能力,相比对照组,服用低剂量SPD后,小鼠体内MDA、H2O2含量降低,NO含量、GSH-PX、T-SOD活性及T-AOC显著提高(P0.05),心肌和血清内MDA含量极显著降低(P0.01),GSH-PX活性极显著提高(P0.01),心肌内表现明显且低剂量优于高剂量,高剂量优于中剂量,低剂量SPD效果虽然优于Vc,但无显著性(P0.05)。3)昆明小鼠经一次性服用中剂量SPD后可提高高温环境下跑台抗氧化能力,相比对照组,服用中剂量SPD后,小鼠体内MDA、H2O2含量降低,NO含量、GSH-PX、T-SOD活性及T-AOC提高,但是无显著差异(P0.05),相比Vc效果无显著差异(P0.05)。4)服用SPD可以对机体氧化代谢水平产生作用,长期服用SPD效果优于一次性服用。研究发现长期服用低剂量SPD可有效提高昆明小鼠在高温环境下运动心肌保护能力,心肌效果优于血清、腓肠肌和肝脏,运动中机体产生的自由基能够快速清除,机体能够及时的抑制或者减轻自由基的链式反应,机体抗氧化能力提高,运动能力提高。
[Abstract]:Objective: To explore the effect of Spermidine (SPD) on the anti oxidation ability of the long-term and one-time high temperature treadmill exercise mice. Methods: 72 male Kunming mice without significant difference were randomly divided into two large groups, one time group and long-term group. Each group was randomly divided into six groups, 12 groups and six groups in each group. They were ONIS (LNIS), Vc group (OHIVc, LHIVc), low dose group (OLSPD, LLSPD) of intensity running platform in high temperature (OLSPD, LLSPD), middle dose group (OTSPD, LTSPD) in high temperature treadmill exercise subspermine (OTSPD, LTSPD), and high dose of high intensity treadmill exercise in high temperature. Group (OHSPD, LHSPD), high temperature OHIS (LHIS) of intensity running table exercise (LHIS), each group of 6, long-term group training for 8 weeks, 5d/w, one day group experiment one day, during the experimental period 10 a.m. gavage, the drug standard is: low, middle, high dose group (0.5mmol/kg, 1mmol/kg, 1.5mmol/kg) in high temperature treadmill exercise; high temperature and normal temperature. The intensity run table exercise physiological saline group (0.86%) and the positive control group high temperature intensity run platform exercise Vc group (1.2g/L). The middle intensity run platform training at two o'clock in the afternoon, the medium intensity is trained at the speed of 12m/min, every day training 10min, the sixth days, the speed of the running platform increases 3m/min, increases to 18m/min stop, and the duration increases 10min in the next day. The room temperature and high temperature room temperature were 23, 39, 39, 50%, 50%, 0 and no weight. Results: 1) after one time and 8 weeks of high temperature treadmill training, it was found that the effects of different doses of SPD on the antioxidant indices of the high temperature mice were different.2 for 8 weeks for 8 weeks. After LHIS compared with LNIS, the level of MDA, H2O2 in the myocardium and liver tissues of Kunming mice increased significantly (P0.05), the activity of GSH-Px and T-SOD and T-AOC decreased significantly (P0.05), and the T-SOD activity in the serum and myocardium of mice decreased significantly (P0.01), and the NO content was not significantly changed. The content of MDA and H2O2 in serum and myocardium decreased significantly (P0.01), the activity of GSH-Px in serum and myocardium increased significantly (P0.01), the content of NO in myocardium increased significantly (P0.05), serum, myocardial and gastrocnemius T-AOC increased significantly (P0.05), MDA in the liver and significantly decreased (P0.05). In contrast, there was no significant difference (P0.05), and the myocardial effect was.3). The intensity run platform training at one time high temperature showed that after OHIS compared with ONIS, it was found that the content of MDA and H2O2 in mice serum, myocardium, liver and gastrocnemius increased significantly (P0.05), GSH-Px and T-SOD activity and T-AOC significantly decreased (P0.05). The content of NO in myocardium and gastrocnemius decreased significantly (P0.05), NO content in the liver was not significantly changed (P0.05); OTSPD compared with OHIS, MDA content decreased significantly (P0.05), OTSPD and OHIVc, no significant difference (P0.05), there was no significant difference between other groups (P0.05). Conclusion: 1) the increase of temperature, after the middle strength of Kunming mice, mice serum, liver, The content of MDA, H2O2, NO content, GSH-PX, T-SOD activity and T-AOC decrease in the myocardium and gastrocnemius muscle. High temperature can increase the level of oxidative metabolism, accelerate the formation of free radicals and accelerate the fatigue rate.2 of the body. After long-term use of low dose SPD in Kunming mice, the antioxidant capacity of long run platform in high temperature environment can be significantly improved, compared with the control group. After low dose of SPD, the content of MDA and H2O2 decreased, NO content, GSH-PX, T-SOD activity and T-AOC significantly increased (P0.05). The MDA content in myocardium and serum was significantly decreased (P0.01), GSH-PX activity was significantly increased (P0.01). The myocardial expression was obvious and the low dose was superior to the high dose, the high dose was superior to the middle dose, and the low dose effect was better although superior to that of the middle dose. Vc, but no significant (P0.05).3) the dose of SPD in Kunming mice could increase the antioxidant capacity in the high temperature environment. Compared with the control group, after the middle dose of SPD, the content of MDA and H2O2 in the mice was reduced, the content of NO, GSH-PX, T-SOD activity and T-AOC were improved, but there was no significant difference (P0.05). Taking SPD can have a effect on the level of the body's oxidation and metabolism, and the effect of long-term use of SPD is superior to that of one-time use. The study found that long term use of low dose SPD can effectively improve the ability to protect the motor myocardium of Kunming mice in high temperature environment. The effect of the myocardium is better than the serum, the gastrocnemius and the liver, the free radicals produced by the body in exercise can be cleared quickly. Besides, the body can inhibit or lighten the chain reaction of free radicals in a timely manner. The body's antioxidant capacity is improved and its athletic ability is improved.
【学位授予单位】：新疆师范大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：G804.2

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