运动训练通过Nrf2对骨骼肌抗氧化蛋白作用的影响
发布时间：2018-08-21 12:28
【摘要】：研究目的:运动训练能够提高机体的抗氧化能力。Nrf2作为机体氧化还原平衡的主要调节因子。运动训练是否会影响Nrf2及抗氧化蛋白表达,进而影响体内氧化还原状态,目前还不清楚。本实验通过对野生小鼠和Nrf2敲除小鼠在安静和运动两种情况下,骨骼肌细胞中Nrf2及抗氧化蛋白表达的检测,探讨运动训练对骨骼肌中Nrf2与抗氧化蛋白表达的影响。研究方法:野生C57BL/6J小鼠和Nrf2敲除小鼠各20只,根据体重,两种鼠分为2组,每组10只,雌雄各半,分别为安静组和运动组,共4组。运动组进行四周跑台运动,每周运动6天,休息一天,每天运动1h,速度12m/min。正式实验前三天,运动组小鼠进行20min适应性的跑台运动。在整个运动过程中未对动物使用任何电刺激。运动组进行最后一次运动之后休息48小时后同安静组同脱颈处死,取材。Western blot法测定骨骼肌胞浆蛋白中4-HNE、Nrf2及抗氧化蛋白含量;RT-PCR测量Nrf2调节的抗氧化基因的表达量;ROS试剂盒测量骨骼肌内ROS含量。研究结果:1)在安静情况下,野生鼠和Nrf2敲除小鼠相比,骨骼肌中ROS以及CAT、NQO1、GCLc、HO-1和SOD2这五个抗氧化蛋白没有显著性改变。2)四周运动训练之后,Nrf2敲除运动组的ROS和大部分抗氧化蛋白都要显著低于野生运动组。研究结论:运动训练通过Nrf2促进小鼠骨骼肌抗氧化基因以及蛋白的表达。
[Abstract]:Objective: exercise training can improve the antioxidant ability of the body. Nrf2 is the main regulatory factor of redox balance. It is not clear whether exercise training will affect the expression of Nrf2 and antioxidant protein, and then the redox state in vivo. The effects of exercise training on the expression of Nrf2 and antioxidant protein in skeletal muscle cells were investigated by detecting the expression of Nrf2 and antioxidant protein in skeletal muscle cells of wild mice and Nrf2 knockout mice. Methods: there were 20 wild C57BL/6J mice and 20 Nrf2 knockout mice. According to their body weight, two kinds of mice were divided into 2 groups, 10 rats in each group, half male and half female, divided into four groups: quiet group and exercise group. The exercise group ran around the platform, 6 days a week, a day off, 1 hour a day, at a speed of 12 m / min. Three days before the formal experiment, the mice in the exercise group underwent 20min adaptive treadmill exercise. No electrical stimulation was used during the whole exercise. The exercise group took a rest for 48 hours after the last exercise and was executed with the same neck removal as the rest group. Determination of 4-HNE-Nrf2 in cytosolic protein of skeletal muscle by Western blot method and determination of antioxidant protein content by RT-PCR the expression of antioxidant gene regulated by Nrf2 was measured by Ros kit. ROS content in skeletal muscle was measured by RT-PCR. Results: 1) in quiet conditions, wild mice were compared with Nrf2 knockout mice. There was no significant change in ROS and SOD2 in skeletal muscle. The ROS and most antioxidant proteins in NRF 2 knockout exercise group were significantly lower than those in wild exercise group. Conclusion: exercise training can promote the expression of antioxidant genes and proteins in skeletal muscle of mice by Nrf2.
【学位授予单位】：北京体育大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：G804.7
本文编号：2195742
[Abstract]:Objective: exercise training can improve the antioxidant ability of the body. Nrf2 is the main regulatory factor of redox balance. It is not clear whether exercise training will affect the expression of Nrf2 and antioxidant protein, and then the redox state in vivo. The effects of exercise training on the expression of Nrf2 and antioxidant protein in skeletal muscle cells were investigated by detecting the expression of Nrf2 and antioxidant protein in skeletal muscle cells of wild mice and Nrf2 knockout mice. Methods: there were 20 wild C57BL/6J mice and 20 Nrf2 knockout mice. According to their body weight, two kinds of mice were divided into 2 groups, 10 rats in each group, half male and half female, divided into four groups: quiet group and exercise group. The exercise group ran around the platform, 6 days a week, a day off, 1 hour a day, at a speed of 12 m / min. Three days before the formal experiment, the mice in the exercise group underwent 20min adaptive treadmill exercise. No electrical stimulation was used during the whole exercise. The exercise group took a rest for 48 hours after the last exercise and was executed with the same neck removal as the rest group. Determination of 4-HNE-Nrf2 in cytosolic protein of skeletal muscle by Western blot method and determination of antioxidant protein content by RT-PCR the expression of antioxidant gene regulated by Nrf2 was measured by Ros kit. ROS content in skeletal muscle was measured by RT-PCR. Results: 1) in quiet conditions, wild mice were compared with Nrf2 knockout mice. There was no significant change in ROS and SOD2 in skeletal muscle. The ROS and most antioxidant proteins in NRF 2 knockout exercise group were significantly lower than those in wild exercise group. Conclusion: exercise training can promote the expression of antioxidant genes and proteins in skeletal muscle of mice by Nrf2.
【学位授予单位】：北京体育大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：G804.7
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