不同BMI的9～12岁男性血清性激素相关指标及脂质组学研究

发布时间：2018-09-01 06:30

【摘要】：研究目的：肥胖的男性儿童青少年表现出性激素水平异常的现象。目前,青春期前男性肥胖与性激素水平的研究受到年龄划分、检测方法、种族差异等的影响而存在一些争议。由于脂代谢常规指标(血脂等)、脂质代谢产物与性激素指标之间均表现出显著的相关关系,因此本研究期望通过了解不同BMI的9～12岁男性的性激素相关指标的差异后,再结合脂质组学来分析9-12岁男性的脂质代谢与性激素相关指标的关系,为儿童青少年肥胖与性激素的研究提供新的思路。研究内容：研究一将了解不同BMI的9～12岁男性脂代谢、性激素相关指标之间的差异。研究二将首次对不同BMI的9～12男性进行血清脂质组学研究,筛选差异脂质代谢物并从脂质组学角度分析男性肥胖与性激素相关指标的关系。研究对象和方法：平均年龄为10.28±0.69岁的72名9～12岁男性被纳入本论文中并根据BMI分为正常组(NC组)、超重(ow组)、肥胖组(OB组)。研究一身体发育指标测试按各标准及仪器规范进行；血清指标测试采用化学发光免疫分析、酶联免疫吸附试验进行；数据处理采用SPSS22.0软件进行分析。研究二采用Waters UPLC/Q-TOF/MS建立脂质组学分析方法：数据采集及差异代谢物鉴定利用MassLynx 4.1软件、Progenesis QI软件、simca-p软件等共同进行分析。研究结果：(1)研究一中Ow组表现为HDL-c降低(p0.01)、性激素结合球蛋白(SHBG)降低(p0.01)、脱氢表雄酮(DHEA)升高(p0.01)的现象,OB组表现为TG升高(p0.01)、HDL-c降低(p0.01)、SHBG降低(p0.01)、DHEA升高(p0.05)的现象。(2)研究二利用UPLC/QTOF/MS仪器建立了不同BMI的9～12岁男性血清脂质组学方法并筛选、鉴定出153个差异脂质代谢物.结论：(1)超重、肥胖的9～12岁男性其性激素相关指标异常的现象表现为DHEA的上升和SHBG的下降。(2)首次对不同BMI的9～12岁男性进行了脂质组学的研究,其中超重肥胖组的脂质代谢紊乱现象主要表现为甘油酯类代谢物的升高、甘油磷脂类和鞘脂类代谢物的下降,这为肥胖及肥胖相关疾病的研究提供了实验依据。
[Abstract]:Objective: obese male children and adolescents showed abnormal sex hormone levels. At present, the study of male obesity and sex hormone level before puberty is influenced by age division, detection method, race difference and so on. Since there is a significant correlation between lipid metabolites and sex hormone indices (lipid, etc.), this study is expected to understand the differences of sex hormone related indexes among 9- and 12-year-old men with different BMI. The relationship between lipid metabolism and sex hormone related indexes in men aged 9-12 years was analyzed in order to provide a new idea for the study of obesity and sex hormone in children and adolescents. Content: the first study will understand the differences of lipid metabolism and sex hormone related indexes among 9-year-old men with different BMI. In the second study, the serum lipids of 9 / 12 men with different BMI were studied for the first time, and the relationship between male obesity and sex hormone related indexes was analyzed from the point of view of lipid group. Participants and methods: 72 912 year old men with an average age of 10.28 卤0.69 years were included in this study and were divided into normal group (NC group), overweight group (ow group) and obese group (OB group) according to BMI. A body development index test was carried out according to each standard and instrument standard; the serum index test was carried out by chemiluminescence immunoassay and enzyme-linked immunosorbent assay (Elisa); and the data processing was analyzed by SPSS22.0 software. In the second study, Waters UPLC/Q-TOF/MS was used to establish the method of lipid analysis: data acquisition and differential metabolites identification were analyzed by MassLynx 4.1 software Progenesis QI software and simca-p software. The results were as follows: (1) in the first Ow group, the HDL-c decreased (p0.01), the sex hormone binding globulin (SHBG) decreased (p0.01), and the dehydroepiandrosterone (DHEA) increased (p0.01). In OB group, the increase of TG (p0.01) and the decrease of HDL-c (p0.01) decreased (p0.01). UPLC/QTOF/MS instrument was used to establish and screen serum lipids of 9- and 12-year-old men with different BMI. 153 different lipid metabolites were identified. Conclusion: (1) the abnormal sex hormone related indexes of 9 ~ 12 years old men who are overweight and obese show that the increase of DHEA and the decrease of SHBG. (2) the lipigraphy of 912 year old men with different BMI was studied for the first time. The disorder of lipid metabolism in overweight and obesity group was mainly characterized by the increase of glycerol ester metabolites, the decrease of glycerol phospholipid and sphingomyelin metabolites, which provided the experimental basis for the study of obesity and obesity related diseases.
【学位授予单位】：北京体育大学
【学位级别】：博士
【学位授予年份】：2016
【分类号】：G804.49

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