耐力训练诱导的肥胖大鼠Irisin抑制脂肪组织FTO表达

发布时间：2018-11-02 17:48

【摘要】：目的:耐力训练引起的高脂膳食诱导肥胖大鼠Irisin水平变化与脂肪组织FTO表达的关系研究。方法:以雄性SD大鼠为研究对象,将大鼠随机分为对照组(C组)、高脂膳食组(HD)、高脂膳食结合运动组(HDE)和运动训练组(CE组),以跑台训练为运动干预手段,坡度为0°,8.2m/min(相当于53%VO2max),第1次运动15min,第2次运动30min,第3次运动45min,第4次以后每次运动60min,共运动8周,每周5次,测定大鼠体重、脂肪重量、血液Irisin含量、骨骼肌FNDC5mRNA和蛋白表达、脂肪组织FTO和UCP1基因mRNA和蛋白表达。结果:高脂膳食显著引起大鼠体重和脂肪含量增加,血液Irisin水平显著降低,骨骼肌FNDC5表达显著降低,脂肪组织FTO表达显著升高而UCP1表达显著降低;耐力训练可以显著降低高脂膳食引起的体重和脂肪含量的增加,显著升高高脂膳食引起的血液Irisin降低,显著升高高脂膳食诱导的骨骼肌FNDC5表达的降低,显著降低高脂膳食引起的脂肪组织FTO表达的升高,而显著升高高脂膳食诱导的UCP1表达的降低;血液中Irisin水平与脂肪组织中FTOmRNA和蛋白呈中度负相关;高脂膳食与高脂膳食结合运动组,骨骼肌中FNDC5mRNA和蛋白与脂肪组织FTOmRNA和蛋白分别呈中度负相关;运动组FNDCmRNA与脂肪组织FTOmRNA无相关,而蛋白呈中度负相关。结论:耐力训练大鼠可能通过释放Irisin,抑制脂肪组织FTO基因表达,进而增强UCP1表达。
[Abstract]:Aim: to study the relationship between the changes of Irisin level and the expression of FTO in adipose tissue of obese rats induced by high fat diet induced by endurance training. Methods: male SD rats were randomly divided into control group (group C), high fat diet group (HD), group) combined with high fat diet group (HDE) group) and exercise training group (CE group). The slope was 0 掳, 8.2m/min (equivalent to 53%VO2max), the first exercise was 15 minutes, the second exercise was 30 minutes, the third exercise was 45 minutes, the fourth time was 60 minutes, the total exercise was 8 weeks, 5 times a week. The weight and fat weight of the rats were measured. Blood Irisin content, skeletal muscle FNDC5mRNA and protein expression, adipose tissue FTO and UCP1 gene mRNA and protein expression. Results: high fat diet significantly increased body weight and fat content, decreased blood Irisin level, significantly decreased FNDC5 expression in skeletal muscle, increased FTO expression in adipose tissue and decreased UCP1 expression in adipose tissue. Endurance training significantly decreased the increase of body weight and fat content induced by high fat diet, significantly increased the decrease of blood Irisin induced by high fat diet, and significantly increased the expression of FNDC5 in skeletal muscle induced by high fat diet. The increase of FTO expression in adipose tissue induced by high fat diet was significantly decreased, while the decrease of UCP1 expression induced by high fat diet was significantly increased. The level of Irisin in blood was negatively correlated with FTOmRNA and protein in adipose tissue, FNDC5mRNA and protein in skeletal muscle were negatively correlated with FTOmRNA and protein in adipose tissue in high fat diet and high fat diet combined exercise group. In exercise group, there was no correlation between FNDCmRNA and FTOmRNA in adipose tissue, but there was a moderate negative correlation between FTOmRNA and protein in exercise group. Conclusion: endurance training rats may inhibit the expression of FTO gene in adipose tissue by releasing Irisin, and then enhance the expression of UCP1.
【作者单位】：山东理工大学体育学院;
【基金】：山东理工大学青年支持计划项目“耐力训练改善肥胖的FTO机制研究”(110026)
【分类号】：G804.2

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