低温雨雪冰冻环境下小鼠运动行为响应及其相关因子调控研究

发布时间：2019-06-21 09:45

【摘要】：目的:研究天然低温雨雪冰冻环境下暴露小鼠运动行为响应及其相关调控因子的变化,进而为该极端环境下如何进行作业保护的研究提供实验依据。方法:挑选40只8周龄健康昆明种雄性小鼠,适应性喂养7天,筛选出符合要求的小鼠随机分为4组:室温环境下安静对照组、运动对照组、低温雨雪冰冻环境下安静实验组和运动实验组。运动实验组和安静实验组每天在低温雨雪冰冻自然环境下暴露1小时。运动实验组和运动对照组在暴露的1,3,5,7天做网屏和衣架实验,用于反映小鼠的肌肉力量和协调能力变化;2,4,6天做吊尾实验,反映小鼠的情绪变化。7天时安静实验组和安静对照组即刻处死取材,运动实验组和运动对照组在末次实验后再做力竭游泳实验(反映小鼠逃生欲望和剩余体能)后处死取材。留取各组小鼠的全脑和骨骼肌备用。检测小鼠骨骼肌和脑的ACh E活性,脑IL-16含量。结果:(1)小鼠运动行为响应:(1)网屏实验:运动实验组滑落时间较运动对照组短,且在5天时滑落时间较长,7天时滑落时间最短。运动对照组在3、5、7天时滑落时间增加。(2)衣架实验:与运动对照组相比,运动实验组分数较小且在5天时分数增加,7天分数时又下降。(3)吊尾实验:与运动对照组相比,运动实验组挣扎时间较短,且随着暴露天数增加挣扎时间下降。(4)力竭游泳实验:与运动对照组相比,运动实验组1分钟游泳速度较大但游泳时间较短。(2)相关调控因子:(1)效应器官ACh E活性:与安静对照组相比,运动对照组骨骼肌ACh E活性较低;与安静实验组相比,运动实验组骨骼肌ACh E活性较低。(2)小鼠脑内ACh E活性:与安静对照组相比,运动对照组、安静实验组ACh E活性均较高;与运动对照组相比,运动实验组脑ACh E活性较低;与安静实验组相比,运动实验组脑内ACh E活性较低。(3)脑内IL-16的含量:与安静对照组相比,运动对照组脑内IL-16的含量较低;与运动对照组相比,运动实验组脑内IL-16的含量较低;与安静实验组相比,运动实验组脑内IL-16的含量较低。结论:1.低温雨雪冰冻环境下小鼠运动行为响应:小鼠肌力减弱,协调性下降,挣扎时间较短,情绪反应降低,游泳实验时第一分钟内逃生欲望增强,但总体运动耐力下降。2.调控因子变化:低温雨雪冰冻环境对小鼠骨骼肌ACh E活性影响不明显,力竭运动会使小鼠骨骼肌ACh E活性降低。低温雨雪冰冻环境使小鼠脑内兴奋性神经递质ACh含量降低;力竭运动使小鼠脑内IL-16含量减少,小鼠免疫功能下降,且低温雨雪冰冻环境下降低更明显。
[Abstract]:Objective: To study the response of exercise behavior and related control factors of exposed mice in the environment of natural low temperature and rain and snow, and to provide the experimental basis for the research on how to carry out the operation protection in the extreme environment. Methods: Forty eight-week-old healthy Kunming male mice were selected, and the mice were randomly divided into 4 groups: a quiet control group, a motion control group, a quiet experimental group and a motion experimental group under the environment of room temperature. The experimental group and the quiet experimental group were exposed to a cold and snow-frozen natural environment for 1 hour each day. The experimental group and the exercise control group were used to screen and coat the exposed 1,3,5,7 days to reflect the changes of muscle strength and coordination ability of the mice. The experiment of the tail-tail was done in 2,4 and 6 days to reflect the changes of the mood of the mice. At 7 days, the experimental group and the quiet control group were killed immediately. The experimental group and the exercise control group were sacrificed after the last experiment and after the exhaustive swimming test (reflecting the desire and remaining physical ability of the mice). The whole brain and skeletal muscle were reserved for each group of mice. The activity of ACh E and the content of IL-16 in skeletal muscle and brain of mice were detected. Results: (1) Mouse exercise response: (1) Screen experiment: The sliding time of the moving experimental group was shorter than that of the control group, and the sliding time was longer than that of the control group at 5 days, and the sliding time at 7 days was the shortest. The sliding time of the control group was increased at 3,5 and 7 days. (2) The experiment of the coat hanger: Compared with the exercise control group, the score of the motion experimental group was small and the score increased at 5 days, and the score of 7 days decreased. (3) Suspension tail experiment: Compared with the exercise control group, the struggle time of the moving experimental group was short, and the struggle time decreased with the number of exposure days. (4) Exhaustive swimming test: Compared with the exercise control group, the swimming time of the exercise group is larger than that of the control group, but the swimming time is short. (2) Relevant regulatory factors: (1) the activity of ACh E in the effect organ: Compared with the control group, the activity of ACh E in the skeletal muscle of the exercise control group was lower; and the activity of ACh E in the skeletal muscle of the experimental group was lower than that of the experimental group. (2) The activity of ACh E in the brain of the mice was higher than that of the control group. The activity of ACh E in the experimental group was lower than that of the control group, and the activity of ACh E in the brain of the experimental group was lower than that of the experimental group. (3) The content of IL-16 in the brain of the control group was lower than that of the control group, the content of IL-16 in the brain of the experimental group was lower than that of the control group, and the content of IL-16 in the brain of the experimental group was lower than that of the experimental group. Conclusion:1. In that low-temperature and snow-frozen environment, the mouse exercise behavior response: the muscle strength of the mouse is weakened, the coordination is reduced, the struggle time is short, the emotional response is reduced, and the escape desire in the first minute is enhanced in the swimming experiment, but the overall movement endurance is reduced. The changes of the control factors: The effect of low-temperature and snow-freezing environment on the activity of ACh E in the skeletal muscle of the mouse was not obvious, and the activity of ACh E in the skeletal muscle of the mouse was reduced by the exhaustive exercise. In that low-temperature and snow-freezing environment, the content of the excitatory neurotransmitter ACh in the brain of the mouse is reduced, the content of IL-16 in the brain of the mouse is reduced by the exhaustive exercise, the immune function of the mouse is reduced, and the decrease of the low-temperature snow and snow freezing environment is more obvious.
【学位授予单位】：沈阳师范大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：G80-05

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