有氧运动结合燕麦β-葡聚糖对高脂仓鼠降低胆固醇的作用及机制

发布时间：2019-06-26 11:04

【摘要】：目的:研究有氧运动结合燕麦β-葡聚糖对高脂仓鼠降低胆固醇的效果,并对有氧运动结合燕麦β-葡聚糖降胆固醇的机制进行探究。方法:以叙利亚金仓鼠为研究对象,喂养高脂饲料构建高脂模型,将造模成功后的仓鼠随机分为高脂饲料对照组(C组)、燕麦β-葡聚糖组(G组),有氧运动组(E组)和有氧运动结合燕麦β-葡聚糖组(GE组),每组10只仓鼠,其中E组和GE组每天进行无负重游泳60 min,按照给食方案喂食仓鼠4周后,检测各组仓鼠进食量、体重和肝脂肪含量,血清中TC、LDL-C、HDL-C和TG含量,肝脏中脂肪含量、TC、FC和TG含量,粪便中脂肪、TC、FC、TG和胆汁酸含量,RT-PCR法检测肝脏中与胆固醇代谢相关的CYP7A1、ABCB11、LDLR、ABCG5和SREBP-2基因的相对表达。结果:与C组相比,GE组高脂仓鼠的体重和肝脂肪含量显著降低;G组、E组和GE组中血清TC、LDL-C含量显著降低,G组和GE组中HDL-C含量显著升高,E组和GE组血清TG含量显著降低,并且,GE组对TC、LDL-C、HDL-C和TG含量的作用效果要明显高于G组和E组;GE组仓鼠的肝脏总脂肪含量显著降低,G组、E组和GE组中仓鼠的肝脏TC含量显著下降,GE组能够显著降低仓鼠肝脏中FC浓度;G组、E组和GE组中仓鼠粪便中的胆汁酸、总脂肪、TG、TC和FC的排泄量均显著高于C组,其中,GE组影响最显著;G组、E组和GE组CYP7A1、ABCB11、CYP51和LDLR的基因表达上调,GE组ABCG5和SREBP-2的基因表达量上调。结论:有氧运动结合燕麦β-葡聚糖比单独进行有氧运动以及单独摄入燕麦β-葡聚糖更能有效降低高脂仓鼠胆固醇;其降低机制是通过调节胆固醇从头合成的限速酶CYP51,胆汁酸合成酶CYP7A1,胆汁酸转运酶ABCB11,胆固醇转运酶ABCG5,LDLR等活性,促进肝脏中胆固醇转化成胆汁酸,增加粪便中脂质物质的排出量,从而使得更多的血浆胆固醇流入肝脏,达到降低血浆胆固醇的水平。
[Abstract]:Objective: To study the effect of aerobic exercise combined with oat-dextran on the lowering of cholesterol in high-fat hamsters, and to explore the mechanism of aerobic exercise combined with oat-glucan-lowering cholesterol. Methods: The high-fat diet was used to construct a high-fat model for high-fat diet, and the successful rats were randomly divided into high-fat diet control group (group C) and oat-dextran group (G group). The aerobic exercise group (group E) and the aerobic exercise combined with the oat-dextran group (GE group), each group of 10 hamsters, wherein the E group and the GE group carry out no weight-bearing swimming for 60 minutes each day, and after feeding the hamsters for 4 weeks according to the feeding scheme, the feeding amount, the body weight and the liver fat content of each group of the hamsters are detected, The relative expression of TC, LDL-C, HDL-C and TG, the content of fat, TC, FC, TG and bile acid in the liver, the content of fat, TC, FC, TG and bile acid in the liver, and the relative expression of CYP7A1, ABCB11, LDLR, ABCG5 and SREBP-2 genes associated with the metabolism of cholesterol in the liver were detected by RT-PCR. Results: Compared with group C, the body weight and liver fat content of GE group were significantly lower than that of group C. The contents of serum TC and LDL-C in group G, E group and GE group were significantly decreased, and the content of HDL-C in group G and GE was significantly increased, and the content of TG in group E and GE group was significantly decreased. The effect of the content of HDL-C and TG was significantly higher than that in group G and E. The content of total fat in the liver of GE group was significantly lower, and the content of TC in the group G, E and GE was significantly lower, and the group of GE could significantly lower the concentration of FC in the liver of the hamster. The amount of bile acid, total fat, TG, TC and FC in the E group and GE group was significantly higher than that of group C, among which the gene expression of the group G, group E and GE group CYP7A1, ABCB11, CYP51 and LDLR was up-regulated, and the gene expression of the GE group ABCG5 and SREBP-2 was up-regulated. Conclusion: The aerobic exercise combined with the oat-glucan is more effective in reducing the cholesterol of the high-fat hamster than that of the oat-glucan alone, and the reduction mechanism is the restriction enzyme CYP51, the bile acid synthetase CYP7A1, The activity of the bile acid transport enzyme ABC11, the cholesterol transport enzyme ABCG5, the LDLR and the like is promoted, the cholesterol in the liver is promoted to be converted into bile acid, and the discharge amount of the lipid substances in the feces is increased, so that more plasma cholesterol flows into the liver, and the level of the plasma cholesterol is reduced.
【作者单位】：山西大学体育学院;
【基金】：教育部人文社会科学基金资助项目(16YJC890006)
【分类号】：G804.7

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