模拟高原训练损伤小肠黏膜屏障引发NF-κB等炎症因子水平上调

发布时间：2019-06-30 22:37

【摘要】：运动员在高原训练中,低氧与运动双重应激中常出现的腹泻、腹痛、食欲不振及其他消化系统紊乱,可能与小肠微观形态受损和伴随发生的细菌增殖异位及炎症有关。本研究构建SD大鼠高原训练模型,观察模拟高原训练对大鼠携氧用氧能力、小肠黏膜屏障完整性、小肠细菌量及小肠NF-κB等炎症因子表达的影响。血液细胞分析结果显示,低氧暴露和低氧训练导致大鼠红细胞数和红细胞压积显著上升。苏木精-伊红染色组织切片证实,低氧暴露导致大鼠小肠机械屏障轻微损伤,低氧训练导致大鼠小肠机械屏障严重受损。荧光原位杂交结果显示,低氧暴露导致大鼠小肠出现细菌增殖发生菌群异位,低氧训练导致增殖和异位现象加剧。蛋白质免疫印迹结果显示,NF-κB仅在低氧暴露和低氧训练组出现高表达,运动组与大鼠对照组无显著差异。逆转录聚合酶链式反应结果显示,TNF-α和IL-6作为NF-κB的下游因子,出现相似的变化趋势。以上结果表明,本研究建立的动物模型可以达到模拟高原训练的目的,高原训练导致大鼠小肠机械屏障被破坏,发生细菌增殖、细菌异位,进而引发小肠炎症因子水平上升。初步揭示了高原训练中运动员出现消化系统不适、腹泻的原因。对于通过补充有益菌的方式平衡菌群,缓解小肠损伤及炎症,解决高原训练中运动员消化系统不适的问题有待继续研究。
[Abstract]:In altitude training, diarrhea, abdominal pain, anorexia and other digestive system disorders often occur in the dual stress of hypoxia and exercise, which may be related to the damage of micromorphology of small intestine and the ectopic proliferation and inflammation of bacteria. In this study, a high altitude training model of SD rats was established to observe the effects of simulated altitude training on oxygen carrying capacity, integrity of intestinal mucosal barrier, bacterial count in small intestine and expression of NF- 魏 B in small intestine. The results of blood cell analysis showed that hypoxia exposure and hypoxia training significantly increased the number of red blood cells and hematocrit in rats. The tissue sections of hematoxylin and eosin staining confirmed that hypoxia exposure caused slight damage to the mechanical barrier of the small intestine in rats, and hypoxia training led to the serious damage of the mechanical barrier of the small intestine in rats. The results of fluorescence in situ hybridization showed that hypoxia exposure led to bacterial proliferation and flora heterosis in the small intestine of rats, and hypoxia training led to the aggravation of proliferation and ectopic phenomenon. Western immunoblotting showed that NF- kappa B was only highly expressed in hypoxia exposure and hypoxia training group, but there was no significant difference between exercise group and rat control group. The results of reverse transcriptional polymerase chain reaction (RT-PCR) showed that TNF- 伪 and IL-6, as downstream factors of NF- 魏 B, showed similar trends. The above results show that the animal model established in this study can achieve the purpose of simulating altitude training. Altitude training leads to the destruction of intestinal mechanical barrier, bacterial proliferation and bacterial heterosis, which leads to the increase of inflammatory factors in the small intestine. The causes of digestive system discomfort and diarrhea in plateau training were preliminarily revealed. It needs to be further studied to balance the flora, alleviate the injury and inflammation of small intestine, and solve the problem of digestive system discomfort in high altitude training.
【作者单位】：北京体育大学体育与健康学部运动人体科学学院;
【基金】：北京体育大学自主科研课题重点课题(No.2015ZD006);北京体育大学自主科研课题博士专项课题(No.2016BS041)资助~~
【分类号】：G804.2

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