不同温湿度条件下滤料微生物繁殖的数值研究
发布时间：2019-03-31 12:41
【摘要】：人们为了获得健康舒适的生活环境已经离不开空调系统(HVAC)。空调系统被广泛应用在各种建筑物内。但是,空调系统本身也会带来环境问题。由于人们平时无法做到定期清洁,许多经过长期使用的空调系统本身又可以作为污染源影响室内空气品质。在适宜的环境条件下,空调系统中的空气过滤器本身就是微生物生长繁殖的温床,这些微生物会产生挥发性化学物质,造成室内环境的二次污染。因此,需要对不同温湿度环境条件下空气过滤器上微生物生长繁殖规律进行研究。本研究不仅进行微生物的培养进而画出生长曲线,还会根据生长曲线选择合适的预测模型进行对应生长参数的预测。首先,本课题为了确定实验中要用到的参数分析了空气处理过程各个环节中空气气流可能的温湿度参数值。然后确定了实验中要采用的参数:使用恒温培养摇床控制15℃、22℃和30℃的恒温条件,使用变色硅胶、以及分别浸润了NaBr的饱和水溶液和生理盐水的脱脂棉来达到恒定的15%、60%和90%的相对湿度条件。接下来,选取F5、F8和H13三个不同过滤等级的玻璃纤维滤料作为研究对象。为了方便实验,将这些滤料样品裁剪为直径8cm的圆形。选取了有代表性的枯草芽孢杆菌作为实验菌种。使用紫外分光光度计测量枯草芽孢杆菌菌悬液的OD(光密度)值以确定菌悬液浓度,并分别取0.2ml的合适浓度的菌悬液,随后用涂布棒涂布的方法,使菌悬液均匀分布于滤料表面。然后放入恒温恒湿环境中连续培养。相对湿度为15%、60%和90%相对湿度条件下对应的微生物的培养周期分别选用1天、7天和7天。将上述经过不同时间培养后的滤料取出,剪碎并洗脱其表面微生物,采用平板计数法来确定洗脱液和对应稀释液中的细菌浓度。最终得到不同温湿度条件下,枯草芽孢杆菌在不同滤料表面的生长繁殖规律。最后,本文还使用Gompertz模型与平方根模型分别建立关于微生物生长的初级模型与二级模型。Gompertz模型模型为初级模型,通过这个模型可以得到不同温湿度下微生物的生长动力参数。平方根模型是二级模型,利用这一模型,研究者能找到在Gompertz模型中得到的最大生长率的平方根与温度的关系。
[Abstract]:In order to get a healthy and comfortable living environment, people can't get away from the air conditioning system (HVAC). Air conditioning system is widely used in various buildings. However, the air-conditioning system itself will also bring environmental problems. Because people can not do regular cleaning, many long-term air conditioning systems themselves can be used as pollution sources to affect indoor air quality. Under suitable environmental conditions, air filters in the air conditioning system are themselves hotbeds for the growth and reproduction of microorganisms, which will produce volatile chemicals and cause secondary pollution to the indoor environment. Therefore, it is necessary to study the growth and propagation of microorganisms in air filter under different temperature and humidity conditions. This study not only carries on the microbial culture and then draws the growth curve, but also chooses the suitable forecast model according to the growth curve to predict the corresponding growth parameters. Firstly, in order to determine the parameters to be used in the experiment, the possible temperature and humidity parameters of the air flow in the air treatment process are analyzed. Then the parameters to be adopted in the experiment were determined: the constant temperature culture shaking bed was used to control the constant temperature conditions of 15 鈩，
本文编号：2450910
[Abstract]:In order to get a healthy and comfortable living environment, people can't get away from the air conditioning system (HVAC). Air conditioning system is widely used in various buildings. However, the air-conditioning system itself will also bring environmental problems. Because people can not do regular cleaning, many long-term air conditioning systems themselves can be used as pollution sources to affect indoor air quality. Under suitable environmental conditions, air filters in the air conditioning system are themselves hotbeds for the growth and reproduction of microorganisms, which will produce volatile chemicals and cause secondary pollution to the indoor environment. Therefore, it is necessary to study the growth and propagation of microorganisms in air filter under different temperature and humidity conditions. This study not only carries on the microbial culture and then draws the growth curve, but also chooses the suitable forecast model according to the growth curve to predict the corresponding growth parameters. Firstly, in order to determine the parameters to be used in the experiment, the possible temperature and humidity parameters of the air flow in the air treatment process are analyzed. Then the parameters to be adopted in the experiment were determined: the constant temperature culture shaking bed was used to control the constant temperature conditions of 15 鈩，
本文编号：2450910
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