中药材二维DNA条形码流通监管体系研究

发布时间：2018-07-10 19:34

本文选题：DNA条形码 + 二维码　；参考：《中国科学:生命科学》2015年07期

【摘要】：中药材品种复杂混乱给中药材流通监管造成不便,加强中药材及饮片质量监管、保证公众用药安全备受关注.本研究将二维DNA条形码技术应用于中药材流通监管领域,建立中药材二维DNA条形码流通监管体系.该体系包括DNA条形码序列获得与DNA条形码信息跨平台转换两大部分内容,以亚麻子、黑芝麻、火麻仁、蓖麻子和苘麻子5种药材共计109份样本为例研究建立该体系:依照中药材DNA条形码分子鉴定法获得待检样本DNA条形码序列;基于开源代码PHP QR Code的编码方式对实验所得DNA条形码序列进行编码,转换成二维码图片;使用移动终端扫描识读该二维码信息,并通过网页浏览器提交至中药材DNA条形码数据库获知鉴定结果.将二维码技术与DNA条形码技术相结合,为每个药材基原物种提供标准二维DNA条形码,有利于DNA条形码信息在不同平台间转换,为二维DNA条形码技术在实际流通监管工作中的应用提供理论基础,能有效实现对中药材流通的数字化监管,推动中药材流通监管现代化、国际化进程.
[Abstract]:The complexity and confusion of Chinese medicinal materials caused inconvenience to the circulation and supervision of Chinese medicinal materials, so strengthening the quality control of Chinese medicinal materials and their pieces and ensuring the safety of drug use by the public have attracted much attention. In this study, two-dimensional DNA barcode technology was applied to the field of Chinese herbal medicine circulation supervision, and the system of two-dimensional DNA bar code circulation supervision was established. The system includes DNA barcode sequence acquisition and cross-platform transformation of DNA barcode information, with linseed, black sesame, fire seed, 109 samples of 5 kinds of medicinal materials of castor and Abutilon theophratheon were studied and established. The DNA barcode sequences of samples were obtained according to the method of DNA barcode molecular identification of Chinese medicinal materials. Based on the coding method of open source code PHP QR Code, the DNA barcode sequence obtained from the experiment is encoded and converted into a two-dimensional code picture, and the two-dimensional code information is scanned and read by the mobile terminal. The identification results were obtained by submitting the web browser to the DNA bar code database of Chinese herbal medicine. Combining the technology of two dimensional code with the technology of DNA bar code, the standard two dimensional DNA bar code is provided for each original species of medicinal material, which is helpful to transform the information of DNA bar code between different platforms. It provides a theoretical basis for the application of two-dimensional DNA barcode technology in the practical circulation supervision, and can effectively realize the digital supervision of the circulation of Chinese medicinal materials, and promote the modernization and internationalization of the circulation supervision of Chinese medicinal materials.
【作者单位】：中国医学科学院北京协和医学院药用植物研究所;中国中医科学院中药研究所;重庆市药物种植研究所;
【基金】：国家自然科学基金(批准号:81373922) 国家科技支撑计划(批准号:2011BAI07B08)资助项目
【分类号】：F203;S567

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