溶藻微生物菌群的富集及其溶藻因素

发布时间：2018-01-02 02:19

本文关键词：溶藻微生物菌群的富集及其溶藻因素　出处：《应用与环境生物学报》2016年06期 　论文类型：期刊论文

【摘要】：为探索行之有效的抑制藻类水华暴发的途径,采集重庆地区蓝藻爆发水域的环境水样,与铜绿微囊藻多次重复共培养富集出具有溶藻能力的高效溶藻液,显示出强而稳定的溶藻能力.通过离心、膜过滤、温度及抗生素因素分析溶藻活性物的主要特性.结果显示:离心处理该高效溶藻液,3 000 r/min离心5 min便可沉淀溶藻活性物,7 000 r/min离心5 min后上清液便失去溶藻能力;膜过滤处理该高效溶藻液,溶藻活性物不能通过2μm的滤膜;温度处理该高效溶藻液,45℃水浴处理10 min即可使其溶藻活性显著降低,48℃水浴处理10 min便可使其完全失去溶藻能力;抗生素处理该高效溶藻液,加入放线菌酮对其溶藻能力没有影响,加入氯霉素对其溶藻能力稍有影响,加入四环素则丧失溶藻能力,抗生素处理结果说明溶藻活性物不是真菌,革兰氏阳性菌的缺失对溶藻活性的影响要大于革兰氏阴性菌;LB培养基分离的单菌落都没有显示出显著的溶藻活性.本研究表明多轮富集的高效溶藻液具有显著稳定的溶藻效果,溶藻液经离心、膜过滤、高温及添加抗生素的结果均显示溶藻因素是细菌,单菌落培养结果说明其溶藻活性依赖于多种细菌组成的微生物菌群共同作用.
[Abstract]:In order to explore the effective way to suppress the algae outbreaks of algae outbreak environmental water samples collected from Chongqing area waters, and Microcystis aeruginosa repeated co cultured with high concentration solution of algicidal algicidal activity, showed a strong and stable algicidal ability. By centrifugation, filtration, and temperature factors are the main characteristics of antibiotics algicidal substance. The results showed that the algicidal centrifugal treatment liquid, 3000 r/min 5 min centrifugal precipitation algicidal substance, 7000 r/min 5 min centrifugal supernatant after losing algae lysing ability; membrane filtration process the efficient algicidal algicidal substance liquid, not through the membrane of 2 m; the high temperature treatment of algicidal liquid, 45 DEG C water bath for 10 min could make the algicidal activity decreased significantly, 48 DEG C water bath for 10 min so that it can be completely lost the algicidal activity; the antibiotic treatment of algicidal liquid, adding cycloheximide on There was no effect on the algae lysing ability, adding chloramphenicol slightly affect their lytic ability, tetracycline is loss of lytic ability, antibiotic treatment results show that the algicidal substance is not lack of fungi, gram positive bacteria influence on algicidal activity than gram negative bacteria; LB culture medium separation single colonies are not displayed the algae activity significantly. This study shows that soluble algicidal liquid rounds of enrichment with algicidal effect was stable, lysing solution by centrifugation and membrane filtration, high temperature and the results of antibiotics showed lytic factors of bacteria, single colony culture shows its algicidal activity dependent microorganisms together acting on a variety of bacteria.

【作者单位】：重庆师范大学生命科学学院;西南大学家蚕基因组生物学国家重点实验室;
【基金】：国家高技术研究发展计划(863计划)项目(2012AA101301) 教育部留学回国人员科研启动基金资助~~
【分类号】：X172;X52
【正文快照】： 水体富营养化所导致的藻类爆发已日益频繁地出现在世界各地的海洋和淡水水域[1],藻类爆发引发一系列水生生态问题[2],藻类大量繁殖和在表层聚集,急剧降低水体中溶解氧的含量,造成大部分好氧生物死亡[3].一些藻类分泌的毒素,如微囊藻分泌的微囊藻毒素[4-7],对包括人在内的所有

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