三株具重金属抗性油菜内生真菌对镉铅锌富集特性研究

发布时间：2018-08-06 16:23

【摘要】：为了筛选到对重金属具良好富集活性的微生物吸附剂,以分离于大宝山矿区重金属富集植物油菜的3株具重金属抗性的内生真菌Fusarium sp.CBRF14,Penicillium sp.CBRF65和Alternaria sp.CBSF68为研究对象,分析了Cd、Pb和Zn的初始浓度对其活菌体的生物量、富集率及富集量的影响。研究结果发现,随着重金属初始浓度(试验设置的浓度范围内)的升高,Fusarium sp.CBRF14、Penicillium sp.CBRF65和Alternaria sp.CBSF68对Cd和Zn的富集率呈下降趋势,而对Pb的富集率则存在"先升高再下降"的过程;该3株内生真菌的生物量随着Cd、Pb和Zn初始浓度增加呈下降趋势,而对Cd、Pb和Zn的富集量则不断增加,它们对Cd的最大富集量介于20.5~53.4 mg·g~(-1),对Pb的最大富集量介于188.9~356.6 mg·g~(-1),对Zn的最大富集量介于28.4~292.8 mg·g~(-1)。研究结果表明这3株油菜内生真菌可在含重金属液体条件下生长并具有良好的重金属富集能力,其在重金属污染废水的生物修复中具有潜在的应用前景,同时也表明重金属耐性植物内生真菌是获得具重金属富集能力微生物的重要资源库。
[Abstract]:In order to screen microbial adsorbents with good enrichment activity for heavy metals, three strains of endophytic fungi, Fusarium sp. CBRF14 Penicillium sp.CBRF65 and Alternaria sp.CBSF68, which were isolated from vegetable oil plants enriched with heavy metals in Dabaoshan mining area, were studied. The effects of the initial concentrations of Pb and Zn on the biomass, enrichment rate and enrichment amount of live bacteria were analyzed. The results showed that the enrichment rates of CD and Zn by Fusarium sp. CBRF14 Penicillium sp.CBRF65 and Alternaria sp.CBSF68 decreased with the increase of the initial concentration of heavy metals (within the range of concentration set in the experiment), while the enrichment rate of Pb increased first and then decreased. The biomass of the three endophytic fungi decreased with the increase of initial CD _ (2 +) Pb and Zn concentrations, but the enrichment of CD _ (2 +) Pb and Zn increased. The maximum concentration of CD, Pb and Zn for CD, Pb and Zn were between 20.5 ~ 53.4 mg / g ~ (-1), 188.9 mg / g ~ (-1) and 28.4 ~ 292.8 mg / g ~ (-1), respectively. The results showed that the three strains of rapeseed endophytic fungi could grow under the condition of liquid containing heavy metals and had good enrichment ability of heavy metals, which had a potential application prospect in bioremediation of heavy metal contaminated wastewater. It is also indicated that endophytic fungi of heavy metal tolerant plants are important resources for obtaining heavy metal enriched microorganisms.
【作者单位】：广东药科大学基础学院;中山大学环境科学与工程学院;
【基金】：国家自然科学基金资助项目(31400111) 广东省自然科学基金资助项目(S2013040016110) 广东省环境污染控制与修复技术重点实验室(中山大学)开放基金资助项目(2013K0005)
【分类号】：X172

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