铜锈环棱螺用于水生态毒理学的基础研究

发布时间：2019-06-21 13:30

【摘要】：铜锈环棱螺作为一种新兴的潜在水生态毒理学模式生物,本论文首先在人为控制条件下,对其室内试验条件进行了优化与筛选,结果表明:当投喂冰鲜小球藻、密度6个/L水体积、水深10-15cm、泥土底质并且水中钙离子浓度30mg·L-1时,铜锈环棱螺的体重增长最为明显。麻醉效果表明,MgCL2·2H2O对成螺的麻醉时间短并且伤害小,可作为实验用麻醉剂首选。本论文随后选取了8个内参基因,分析了经为期10天暴露于3种不同雌激素下,铜锈环棱螺内脏组织内参基因的表达水平,利用RefFinder在线分析了各个内参基因在铜锈环棱螺不同雌激素暴露下体内表达水平的稳定性,结果表明,糖体蛋白L7(RPL7)的表达最为稳定。因此,RPL7可作为铜锈环棱螺在不同外源环境条件刺激下,定量目标基因表达水平的合适内参基因之一。最后,针对太湖流域铜锈环棱螺的性比进行了初步调查,发现其畸变率较高,左右触角或右侧触角呈分叉状或蹼状的畸变发生率约为2.7%,对畸变及正常成螺进行了初步的组织学研究,发现肌肉组织含有大量寄生虫胚胎,正常组织中,腹足肌肉为平滑肌,精巢内含大量成熟簇状精子,属进化型的II型精子。以上研究结果皆为发展以铜锈环棱螺为指示生物开展的水生态环境毒理学研究奠定了基础。
[Abstract]:As a new potential aquatic ecotoxicological model organism, the laboratory test conditions were optimized and screened under the condition of artificial control. The results showed that when Chlorella vulgaris was fed with chlorella, density 6 / L water volume, water depth 10-15 cm, soil sediment and calcium concentration in water 30mg 路L-1, the body weight of Pterus cerevisiae increased the most obviously. The results showed that when Chlorella vulgaris was fed with chlorella vulgaris, the water depth was 10-15 cm, the soil sediment and the concentration of Ca ~ (2 +) in water were 10 ~ (- 15) cm. The anesthetic effect showed that MgCL2 路2H2O had short anesthetic time and little injury to adult snail, so it could be used as the first choice for experimental anaesthesia. In this paper, eight internal reference genes were selected to analyze the expression level of internal reference genes in visceral tissues of three different estrogens exposed to three different estrogens for 10 days. RefFinder was used to analyze the stability of the expression levels of each internal reference genes in different estrogens exposed to different estrogens. The results showed that the expression of glycosome protein L7 (RPL7) was the most stable. Therefore, RPL7 can be used as one of the suitable internal reference genes for quantitative target gene expression under different external environmental conditions. Finally, the sex ratio of copper rust snail in Taihu Lake Basin was investigated. It was found that the distortion rate was high, and the incidence of forked or webbed distortion in left and right tentacles was about 2.7%. A preliminary histologic study on distortion and normal snail was carried out. It was found that muscle tissue contained a large number of parasite embryos. In normal tissue, gastropoda muscle was smooth muscle and sperm nest contained a large number of mature cluster sperm. Evolutionary type II sperm. The above results laid a foundation for the development of aquatic ecological environment toxicology research with copper rust ring snail as an indicator organism.
【学位授予单位】：天津农学院
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：X171.5

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