基于喷墨打印技术的生物DVD光盘的制备与检测

发布时间：2018-07-09 15:47

本文选题：即时在线检测 + DVD光盘　；参考：《太原理工大学》2013年硕士论文

【摘要】：微阵列技术作为一种强大的生物检测与分析方法,近年来得到了广泛的关注和研究,但是由于生物芯片点样仪和激光荧光扫描仪价格昂贵,致使微阵列技术的应用仍局限于大型医院和专业的生物实验室,而无法得到广泛推广。研发低成本、易操作的生物分子快速、定量分析方法对于提高公共健康质量,尤其是疾病的早期和在线诊断(point-of-care, POC)具有非常重要的意义。随着喷墨打印机的广泛应用,以及计算机光驱/光盘的全面普及,利用喷墨打印技术在光盘塑料基底上制备多通道微阵列,同时结合光驱／光盘的分子检测技术,有望成为新一代具有应用前景的医疗诊断工具。本文主要研究了利用喷墨打印机制备生物检测DVD光盘,并且利用普通光驱信号识别方法进行了定量检测。值得一提的是,研究中所使用的仪器均为大量普及的商用产品,因此该方法最大的优势就是检测成本低、操作简单、易于推广。本论文主要完成以下工作： 1.成功地将DVD光盘表面聚碳酸酯材料由疏水性改变为亲水性。经过紫外臭氧处理后,水在其表面的接触角从88.07。减小到9.33。。 2.实验制备了PDMS微流体通道,利用它在光盘表面限制生物素-链酶亲和素免疫反应区域,最后通过银染使检测区域内的探测信号放大。利用光盘纠错分析软件将制备好的DVD生物光盘进行扫描和数据读取,通过分析单位面积内的错误数和光学灰度值,进行定量检测。与CD生物检测光盘相比,DVD生物检测光盘具有更高的灵敏度。 3.对未经改造的商用压电式喷墨打印机进行了色彩管理测试和墨水扩散分析,并且利用喷“墨”打印方法在DVD光盘表面制备不同浓度的多通道生物素-链酶亲和素免疫微阵列结构。研究表明,通过这种制备方法对链酶亲和素的检测灵敏度为0.6μg/mL,检测范围在0.6-1.2μg/mL。实验也表明打印墨盒所需试剂最小容量为500μL,最佳打印次数为5次。
[Abstract]:As a powerful biological detection and analysis method, microarray technology has been widely studied in recent years, but because of the high price of biochip spotting instrument and laser fluorescence scanner, As a result, the application of microarray technology is still limited to large hospitals and professional biological laboratories, but can not be widely spread. Research and development of low-cost and easy-to-operate biomolecules are very important for improving public health quality, especially for point-of-care (POC). With the wide application of inkjet printer and the popularization of computer optical drive / optical disk, multi-channel microarrays are fabricated on the plastic substrate of optical disk by using inkjet printing technology, and the molecular detection technology of optical drive / optical disk is combined at the same time. It is expected to be a new generation of medical diagnostic tools with application prospects. In this paper, we mainly study the preparation of biological detection DVD disc by ink jet printer, and use the conventional optical drive signal recognition method to carry out quantitative detection. It is worth mentioning that the instruments used in the research are a large number of popular commercial products, so the biggest advantage of this method is that the detection cost is low, the operation is simple, and it is easy to be popularized. The main work of this thesis is as follows: 1. The surface polycarbonate of DVD disc was changed from hydrophobic to hydrophilic. After UV ozone treatment, the contact angle of water on its surface is from 88.07. Reduced to 9.33.2. PDMS microfluidic channels were prepared, which were used to limit the immunoreactive region of biotin chain enzyme on the optical disk surface. Finally, the detection signal in the detection region was amplified by silver staining. The prepared DVD biological disc is scanned and read by using the optical disk error correction analysis software. The error number and optical gray value in unit area are analyzed for quantitative detection. Compared with CD biological detection disc, DVD biological detection disc has higher sensitivity. 3. 3. The color management test and ink diffusion analysis of unmodified commercial piezoelectric inkjet printer are carried out. And the multi-channel biotin-chain enzyme immuno-microarray structure with different concentration was prepared on the DVD disc surface by using the "ink" printing method. The results showed that the sensitivity of the method was 0.6 渭 g / mL and the detection range was 0.6-1.2 渭 g / mL. The experiment also shows that the minimum capacity of reagents needed for printing cartridges is 500 渭 L and the optimum printing times are 5 times.
【学位授予单位】：太原理工大学
【学位级别】：硕士
【学位授予年份】：2013
【分类号】：TP334.8;TP333.4

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