转双价抗虫基因大白菜新种质的获得及其抗虫性分析

发布时间：2018-01-07 00:18

本文关键词：转双价抗虫基因大白菜新种质的获得及其抗虫性分析　出处：《西北植物学报》2016年01期 　论文类型：期刊论文

【摘要】：采用超声波辅助花粉介导方法,将双价抗虫基因BmkIT-Chitinase导入早熟型大白菜自交系20-19-3,最终获得了5个转基因大白菜优良自交系纯合株系Z1-5、Z2-7、Z9-6、Z11-6和Z20-13;以转基因大白菜株系和非转基因对照植株为材料,对BmkIT-Chitinase基因在大白菜中的遗传规律、基因表达及抗虫性进行进一步分析。结果显示:(1)转化株后代多代(T_1~T_4)PCR、Southern blotting等分子跟踪检测表明,目的基因已成功导入受体植株,且能够稳定遗传;用该转基因方法对大白菜进行基因转化所获得的转基因植株分析显示,外源基因多数以多拷贝形式整合于核基因组,少部分外源基因以单拷贝形式整合。(2)Elisa分析结果证明,所导入的外源基因可高效表达,T4代株系新鲜叶片中表达产物量最高达到0.069μg·g~(-1)左右。(3)转基因株系田间抗虫性统计分析表明,转化株系与对照在抗虫性方面有显著差异,其对小菜蛾及菜青虫抗性普遍提高2~3级。研究认为,转BmkIT-Chitinase基因大白菜中BmkIT-Chitinase基因的表达可有效提高大白菜的抗虫性。
[Abstract]:The bivalent insect-resistant gene BmkIT-Chitinase was introduced into early maturing Chinese cabbage inbred line 20-19-3 by ultrasonic assisted pollen mediated method. Finally, five excellent inbred lines of transgenic Chinese cabbage Z1-5Z2-7Z9-6Z11-6 and Z20-13were obtained. The inheritance of BmkIT-Chitinase gene in Chinese cabbage was studied by using transgenic Chinese cabbage lines and non-transgenic control plants as materials. Gene expression and insecticidal resistance were further analyzed. Southern blotting and other molecular tracking tests showed that the target gene had been successfully introduced into the recipient plants and could be inherited stably. The analysis of transgenic plants obtained from transgenic Chinese cabbage showed that most of the foreign genes were integrated into the nuclear genome in the form of multiple copies. A small number of foreign genes were integrated by single copy. The results of Elisa analysis showed that the imported foreign genes could be expressed efficiently. The highest amount of expression products in fresh leaves of T4 generation was about 0.069 渭 g 路g ~ (-1) ~ (-1). The statistical analysis of insecticidal resistance of transgenic lines in the field showed that the transgenic lines were resistant to insect pests in the field. The resistance of transformed lines to Plutella xylostella and cauliflower caterpillar increased by 2 ~ 3. The results showed that the resistance of transformed lines to diamondback moth and cabbage caterpillar was significantly higher than that of control strain. The expression of BmkIT-Chitinase gene in transgenic Chinese cabbage with BmkIT-Chitinase gene can effectively improve the insecticidal resistance of Chinese cabbage.
【作者单位】：山西省农业科学院蔬菜研究所;新疆建设兵团第六师农业科学研究所;
【基金】：科技援疆项目(2014072008)
【分类号】：S436.341
【正文快照】： 大白菜(Brassica campestris L.ssp.pekinen-sis)起源于中国,又称结球白菜、白菜等,是中国乃至亚洲重要的栽培食用蔬菜之一。由于其富含维生素、糖、矿物质等营养成分,深受广大消费者喜食。但在大白菜的栽培过程中,虫害一直是影响其高产、稳产及品质的重要制约因素,其中,菜青

【相似文献】