结直肠癌患者粪便p16INK4a、MGMT、RASSF1A基因甲基化状态观察

发布时间：2018-01-24 23:59

本文关键词： 结直肠癌 pINKa基因 MGMT基因 RASSFA基因基因甲基化　出处：《山东医药》2017年31期 　论文类型：期刊论文

【摘要】：目的观察结直肠癌(CRC)患者粪便p16INK4a、MGMT、RASSF1A基因甲基化状态。方法 CRC患者50例(肿瘤组)、结直肠良性病变者50例(良性组)、健康体检者50例(正常组),采用甲基化特异度PCR的方法检测各组粪便p16INK4a、MGMT、RASSF1A基因甲基化检出率,并分析其与CRC临床病理参数的关系。根据肠镜病理诊断结果进行验证,比较粪便p16INK4a、MGMT、RASSF1A单独及联合检测诊断CRC的敏感度和特异度。结果肿瘤组、良性组、正常组粪便p16INK4a基因甲基化检出率分别为74%、28%、14%,MGMT基因甲基化检出率分别为56%、24%、12%,RASSF1A基因甲基化检出率分别为72%、20%、10%,肿瘤组分别与正常组、良性组比较,P均0.05。p16INK4a基因甲基化状态与CRC分化程度、TNM分期、淋巴结转移相关,MGMT基因甲基化状态与CRC淋巴结转移相关,RASSF1A基因甲基化状态与CRC分化程度、TNM分期、淋巴结转移相关,P均0.05。三者联合检测诊断CRC的敏感度为95.8%,特异度为83.4%,ROC曲线下面积为0.816(95%CI 0.739%~0.894%)。结论 CRC患者粪便p16INK4a、MGMT、RASSF1A基因甲基化检出率明显高于结直肠良性病变者及健康体检者,联合检测上述指标有助于CRC患者的早期诊断及其生物学行为的判断。
[Abstract]:Objective to observe the methylation status of p16INK4a MGMT-RASSF1A gene in feces of patients with colorectal cancer. Methods 50 patients with CRC (tumor group) were enrolled in this study. Fifty patients with colorectal benign lesions (benign group) and 50 healthy persons (normal group) were detected by methylation specificity (PCR) method. The detection rate of methylation of RASSF1A gene and its relationship with the clinicopathological parameters of CRC were analyzed. The stool p16INK4a MGMT was compared according to the results of pathological diagnosis under enteroscopy. Results the detection rate of methylation of p16INK4a gene in feces of tumor group, benign group and normal group was 7428%. The positive rate of methylation of MGMT gene was 56%, and the positive rate of methylation of RASSF1A gene was 72%, 20% and 10%, respectively. The methylation status of p16INK4a gene was correlated with CRC differentiation and lymph node metastasis. The methylation status of MGMT gene was associated with CRC lymph node metastasis. The methylation status of RASSF1A gene was correlated with the degree of CRC differentiation and lymph node metastasis. P all 0.05. The sensitivity and specificity of combined detection and diagnosis of CRC were 95.8 and 83.4% respectively. The area under the ROC curve was 0.816 ~ 95% CI 0.739 and 0.8944.Conclusion the stool p16INK4a of CRC patients has MGMT. The detection rate of methylation of RASSF1A gene was significantly higher than that of benign colorectal lesions and healthy persons. The combined detection of the above indexes was helpful to the early diagnosis and the judgement of biological behavior of patients with CRC.
【作者单位】：惠州市中心人民医院;
【基金】：广东省惠州市民政局资助项目(20151116344)
【分类号】：R735.34
【正文快照】： 结直肠癌(CRC)是常见的恶性消化道肿瘤之一,有较高的发病率及病死率[1,2]。CRC早期未发生癌组织转移时患者5年生存率为90%,发生局部转移时患者生存率为68%,发生远处转移时患者生存率仅为10%[3~5]。早发现及早治疗能明显提高CRC患者的生存率[6],近年发达国家的CRC患者5年生存率

【相似文献】