肥胖对小鼠精子全基因组DNA甲基化以及基因印迹的影响

发布时间：2018-01-31 22:18

本文关键词： 肥胖精子基因印迹全基因组甲基化　出处：《中华男科学杂志》2017年06期 　论文类型：期刊论文

【摘要】：目的:研究高脂饲料诱导的肥胖是否影响小鼠精子印迹基因差异甲基化区域和精子全基因组DNA甲基化水平。方法:利用高脂饲料诱导的肥胖小鼠模型,进行精子DNA的全基因组甲基化和印迹基因差异甲基化区测序。结果:高脂饲料诱导的肥胖组印迹基因差异甲基化区甲基化水平与普通饲料对照组无显著差异:MEG3-IG(93.73%vs 97.26%;P=0.252),H19(98.00%vs 97.83%,P=0.920),IGF2(97.34%vs 96.25%,P=0.166),IGF2R(1.43%vs 1.11%,P=0.695),PEG3(0.19%vs 0.38%,P=0.537),MEST(0.23%vs 0.68%,P=0.315),NNAT(0.31%vs 0.00%,P=0.134)和SNRPN(1.88%vs 3.13%,P=0.628)。精子全基因组范围内总共发现8 942个甲基化有显著差异的区域(P0.05)。对差异甲基化区域进行基因功能富集,富集基因数目最多的3项分别为代谢(n=1 482),RNA合成(n=779)和转录(n=767)。结论:高脂饲料诱导的肥胖小鼠精子印迹基因差异甲基化区甲基化水平没有发生显著改变,参与代谢、RNA合成以及转录过程基因CG序列甲基化显著改变。
[Abstract]:Objective: to study whether the obesity induced by high fat diet affects the differential methylation region of sperm imprinting gene and the level of DNA methylation of sperm genome in mice. Methods: the obese mice model was induced by high fat diet. Genomic methylation and differential methylation of imprinted genes in sperm DNA were sequenced. Results:. There was no significant difference in methylation level between high fat diet induced obesity group and normal diet control group. 93.73 vs 97.26; (P = 0.252 / h ~ (98.00) vs 97.83 / P ~ (0.920) IGF _ (2) 97.34 vs 96.25 / P = 0.166). IGF2R(1.43%vs 1.11 / 0.695PEG3 0.19 vs 0.38: P0.537). MEST(0.23%vs 0.68 was 0.315 and 0.31 vs 0.00%. P0. 134) and SNRPN(1.88%vs 3.13%. A total of 8 942 regions with significant difference in methylation were found in the whole genome of spermatozoa (P0. 05). The gene function of differential methylation region was enriched. The three most abundant genes were Metabolism nu 1 482). Conclusion: the methylation level of differential methylation region of sperm imprinting gene in obese mice induced by high fat diet has no significant change and is involved in metabolism. RNA synthesis and CG sequence methylation of transcriptional process genes were significantly altered.
【作者单位】：北京大学第三医院妇产科生殖医学中心;河北大学医学部;北京大学第三医院泌尿外科;
【基金】：国家自然科学基金青年基金(81300483)~~
【分类号】：R589.2
【正文快照】： 肥胖是先天或后天营养失衡导致的伴有代谢紊启动高保真酶。乱的常见疾病。据卫生和计划生育委员会发布的1.2 DIO小鼠模型建立3周龄C57BL6/J雄鼠,《中国居民营养与慢性病状况报告(2015年)》统每4只分笼饲养,肥胖组饲喂高脂饲料D12492计:全国18岁及以上成人超重率为30.1%,肥胖率

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