1700008O03Rik基因在小鼠睾丸中的表达特征及生物信息学分析

发布时间：2018-01-31 22:19

本文关键词： ORik基因精子发生睾丸小鼠　出处：《中华男科学杂志》2017年03期 　论文类型：期刊论文

【摘要】：目的:研究1700008O03Rik(RIKEN cDNA 1700008O03)基因在B6小鼠睾丸中从出生到性成熟过程的表达特征,并初步探讨其参与调控小鼠精子发生的作用。方法:小鼠基因表达谱芯片筛选得到睾丸特异性基因1700008O03Rik,本研究采用反转录PCR、实时定量PCR、Western印迹、免疫组化及免疫荧光等实验方法,检测1700008O03Rik基因在小鼠睾丸发育中的表达特征,并用生物信息学方法作相关分析。结果:1700008O03Rik基因在小鼠睾丸中高表达,且呈鼠龄依赖性增加;免疫组化结果表明其主要表达于长形精子胞浆。生物信息学分析结果显示人和小鼠的1700008O03Rik蛋白序列高度同源,进化树分析结果表明从低等哺乳动物到高等哺乳动物1700008O03Rik蛋白均高度保守。结论:1700008O03Rik为小鼠睾丸高表达基因,且主要表达于长形精子胞浆,其可能参与调控小鼠精子的成熟过程。
[Abstract]:Objective: to study the expression of RIKEN cDNA 1700008O03 gene in the testis of B6 mice from birth to sexual maturation. Methods: testicular specific gene 17008O03Rik was obtained by microarray screening of mouse gene expression profile. Reverse transcription PCR was used in this study. The expression of 17008O03Rik gene in mouse testis was detected by Western blot, immunohistochemistry and immunofluorescence. The results showed that: 1 700 008 O 03 Rik gene was overexpressed in mouse testis and increased in an age-dependent manner. The results of immunohistochemistry showed that it was mainly expressed in the long sperm cytoplasm. Bioinformatics analysis showed that the sequence of 17008 O03Rik protein in human and mouse was highly homologous. The result of phylogenetic tree analysis showed that 1700008O03Rik protein was highly conserved from lower mammal to higher mammal. 1700008O03Rik is a highly expressed gene in mouse testis. And mainly expressed in the long sperm cytoplasm, which may be involved in the regulation of mouse sperm maturation process.
【作者单位】：广州医科大学研究生院;北京大学深圳医院深圳市男性生殖与遗传重点实验室;
【基金】：深圳市科技计划项目(JCYJ20130402113131202,JCYJ20140415162543017)~~
【分类号】：R698.2
【正文快照】： 不孕不育严重危害人类身心健康,WHO已经将其与肿瘤和心脑血管疾病并称为危害人类身心健康的三大病因。据不完全统计,从上个世纪初开始,男性精液的质量呈现出逐年下降的趋势,男性生殖健康的问题已经受到国内外医学界的重点关注。但男性不育的致病因素错综复杂,很多疾病或者因素

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