DNA损伤修复基因多态性与膀胱癌预后的相关性

发布时间：2018-01-31 23:04

本文关键词： 膀胱癌 DNA损伤修复基因单核苷酸多态性预后　出处：《兰州大学》2017年硕士论文　论文类型：学位论文

【摘要】：目的:DNA损伤修复基因在癌症的发生发展过程中起着至关重要的作用。本课题对我国人群7个DNA损伤修复基因中32个单核苷酸多态性位点(SNP)在膀胱癌临床发生发展过程中所起的作用及与预后之间的关系进行研究。方法:应用TaqMan OpenArray基因芯片技术对我国227名膀胱癌患者进行了高通量基因分型检测,分析其与膀胱癌预后相关因素的关系。结果:在有吸烟习惯的膀胱癌患者中APEX1基因rs1130409位点SNP与膀胱癌的易感性相关(P0.001);XRCC1基因的rs3213356位点、APEX1基因rs3136817位点以及MUTYH基因的rs3219493位点不同SNP即变异型与野生纯合子与膀胱癌的临床病理特征具有相关性(P0.001;P0.001;P0.001);APEX1基因rs3136817位点和XRCC2基因rs3218454位点SNP可预测患者复发风险(P0.001;P0.001),常见于变异基因型当中。PARP1基因rs1805415位点的变异基因型相比野生型,生存时间相对较长(P=0.02;P=0.04)。结论:DNA损伤修复基因多个单核苷酸多态性位点膀胱癌患者的临床病理特征和预后具有显著相关性。
[Abstract]:Objective to study the role of DNA damage repair gene in the development of cancer. This study has been applied to 32 single nucleotide polymorphisms (SNPs) of 7 DNA damage repair genes in Chinese population. Methods: to study the role of TaqMan in the clinical development of bladder cancer and its relationship with prognosis. High-throughput genotyping of 227 bladder cancer patients in China was detected by OpenArray gene chip technique. Results: in patients with bladder cancer with smoking habit, the SNP of rs1130409 locus of APEX1 gene was associated with the susceptibility of bladder cancer. P0.001; The rs3213356 locus of XRCC1 gene. The clinicopathological characteristics of APEX1 gene rs3136817 locus and MUTYH gene rs3219493 locus are correlated with the clinicopathological characteristics of bladder cancer. (. P0.001; P0.001; P0.001; SNP at rs3136817 site of APEX1 gene and rs3218454 locus of XRCC2 gene could predict the recurrence risk of patients (P0.001). P0.001A, the survival time of the variant genotypes in the rs1805415 locus of the parP1 gene was longer than that of the wild genotype. The survival time of P0. 02 was longer than that of the wild genotype at the rs1805415 locus of PARP1 gene. Conclusion there is a significant correlation between clinicopathological features and prognosis of bladder cancer patients with multiple single nucleotide polymorphisms of DNA damage repair gene.
【学位授予单位】：兰州大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R737.14

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