不同品种甜高粱SS基因表达与糖分积累关系的研究

发布时间：2018-02-01 13:02

本文关键词： 甜高粱含糖量蔗糖合成酶荧光定量PCR　出处：《沈阳师范大学》2017年硕士论文　论文类型：学位论文

【摘要】：甜高粱是新一代能源作物,是生产乙醇燃料的重要原料,甜高粱含糖量是决定乙醇产量的主要因素,提高甜高粱含糖量是当今甜高粱育种最重要的研究内容之一。本实验选取了国内外10个甜高粱主栽品种,比较不同品种甜高粱不同时期的糖含量以及蔗糖合成酶和蔗糖磷酸合成酶的活性变化,利用实时荧光定量PCR技术比较4个时期、10个甜高粱品种之间SS基因表达的差异,了解甜高粱体内糖分累积规律,主要结果如下:1、在不同的生长时期蔗糖含量和总糖含量变化规律比较一致,均呈逐渐上升趋势,在成熟期时达到峰值。果糖和葡萄糖含量的变化趋势与总糖的变化趋势不同,果糖含量的变化整体上呈现升高、降低再增高的变化趋势,葡萄糖含量总体上是呈现出增高、降低的变化趋势。对甜高粱叶片中的糖含量进行相关性分析,结果得出,叶片中总糖含量与蔗糖含量呈显著正相关(R=0.632,P0.01),总糖含量与果糖含量无相关性(R=0.227,P=0.159),总糖含量与葡萄糖含量呈负相关(R=-0.328,P0.05)。2、叶片中SS活性和SPS活性的变化趋势大体相同,整体上呈现上升的趋势,成熟期达到最大值,叶片中SS的酶活性大于SPS酶活性。在生长前期时,叶片中SS和SPS酶活性存在差别不大,在糖类积累的生长后期,SS和SPS酶活性才表现出明显的差别。叶片中蔗糖含量与SS酶活性呈正相关(R=0.860,P0.05),与SPS酶活性也呈正相关(R=0.876,P0.05),说明SS和SPS是影响甜高粱蔗糖积累过程的主要的酶。在成熟期时品种LTR108的SS酶活性和SPS酶活性均为最大。3、通过对叶片中SS基因相对表达量定量分析可知,在成熟时期SS基因相对表达量最高的是LTR108这一品种,这样的表达差异与蔗糖含量差异一致,说明甜高粱SS基因的表达量是影响蔗糖含量的因素,从而导致不同品种的甜高粱之间蔗糖含量的不同。
[Abstract]:Sweet sorghum is a new generation of energy crops, is an important raw material for the production of fuel ethanol, sugar content of sweet sorghum is the main factor to determine the ethanol yield, increase the sugar content of sweet sorghum is one of the most important research content in sweet sorghum breeding. This experiment selects 10 domestic and foreign sweet sorghum varieties, comparison of sugar content in different periods of different varieties of sweet sorghum and the changes of the activity of sucrose synthase and sucrose phosphate synthase, using real-time fluorescence quantitative PCR technology 4 times, the difference of SS gene expression between 10 varieties of sweet sorghum, sweet sorghum sugar accumulation in understanding, the main results are as follows: 1, compared with the variation in different growth periods of sucrose content and total sugar content were. Increased gradually, reached a peak at mature stage. The different trend of the content of fructose and glucose and total sugar, fructose content change The overall trend of increased, decreased and then increased, the content of glucose in general are showing increased, the trend of decrease. By correlation analysis of sugar content of sweet sorghum leaves in the leaves of total sugar content and sucrose content showed a significant positive correlation (R=0.632, P0.01), there was no correlation between total sugar content with the content of fructose (R=0.227, P=0.159), the total sugar content was negatively correlated with the content of glucose (R=-0.328, P0.05).2, the change trend of SS activity and SPS activity in the leaves of roughly the same, the overall upward trend, the maturity stage and reached the maximum enzyme activity in leaf of SS was greater than SPS in enzyme activity. The early growth stage, SS and SPS activity in leaves has little difference, carbohydrate accumulation in late growth stage, SS and SPS activity did not show significant differences. In the leaves of sucrose content and SS enzymatic activity was positively correlated (R=0.860, P0.05), and SPS activity. It was positively correlated (R=0.876, P0.05), SS and SPS are the main enzymes of sucrose accumulation. In the mature stage of SS enzyme activity and SPS activity of varieties of LTR108 were the highest in the leaves of SS.3, based on the analysis of relative expression quantity of SS gene, in the mature period of relative the expression of LTR108 was the highest of the varieties, consistent differences expressed this with sucrose content, indicating the expression of SS gene is the influence factors of sweet sorghum sucrose content, resulting in different varieties of sweet sorghum sucrose content is different.

【学位授予单位】：沈阳师范大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：S566.5

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