用保幼激素和蜕皮激素处理离体培养家蚕丝腺对丝蛋白基因表达的影响

发布时间：2018-03-07 01:10

本文选题：家蚕丝腺　切入点：体外培养　出处：《蚕业科学》2017年03期 　论文类型：期刊论文

【摘要】：蜕皮激素(20E)和保幼激素(JH)具有共同调控家蚕丝蛋白合成的作用。将家蚕5龄第3天幼虫的中部和后部丝腺置于含不同浓度20E或JH的培养基中进行离体培养,于培养不同时间通过定量PCR检测丝素蛋白基因及丝胶蛋白基因的表达变化。结果表明,蜕皮激素处理对丝蛋白基因表达的影响,因激素使用剂量和作用时间不同而异;保幼激素处理会抑制丝蛋白基因的表达,尤其对丝素蛋白重链基因Bmfib-H的表达抑制作用更为明显,50 ng/m L JH处理48 h后Bmfib-H的表达水平下调1倍左右;蜕皮激素和保幼激素共同处理比单一激素处理更有助于丝蛋白基因的表达,但用保幼激素先处理丝腺24 h后再用蜕皮激素和保幼激素共同处理,会显著抑制丝素蛋白轻链基因Bmfib-L的表达。试验在排除蚕体内源激素干扰的条件下,从转录水平初步分析了保幼激素和蜕皮激素对家蚕丝蛋白基因表达的调控机制。
[Abstract]:The central and posterior silk glands of Bombyx mori larvae on the 3rd day of 5th instar were cultured in vitro in a medium containing different concentrations of 20E or JH. The expression of silk fibroin gene and sericin gene was detected by quantitative PCR at different time of culture. The results showed that the effect of ecdysone treatment on the expression of silk protein gene varied with the dosage and time of hormone use. Juvenile hormone treatment inhibited the expression of silk protein gene, especially the expression of silk protein heavy chain gene (Bmfib-H). The expression level of Bmfib-H was down-regulated by about 1 fold after 48 h treatment with 50 ng/m L JH. The co-treatment of ecdysone and juvenile hormone was more helpful to the expression of silk protein gene than that of single hormone treatment. However, the silk gland was treated with juvenile hormone for 24 hours and then treated with ecdysone and juvenile hormone. The Bmfib-L expression of silk fibroin light chain gene was significantly inhibited. The regulation mechanism of juvenile hormone and ecdysone on the expression of silk protein gene in silkworm was preliminarily analyzed from the transcriptional level.
【作者单位】：苏州大学基础医学与生命科学学院;现代丝绸国家工程实验室;
【基金】：现代农业产业技术体系建设专项(No.CARS-22)
【分类号】：S881.2

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