小麦锈菌碳水化合物酶类基因家族的鉴定与进化分析

发布时间：2018-03-09 17:53

本文选题：小麦锈菌　切入点：碳水化合物酶类　出处：《菌物学报》2017年09期 　论文类型：期刊论文

【摘要】：本研究利用已测序的条形柄锈菌Puccinia striiformis f.sp.tritici基因组序列信息,结合已公布的隐匿柄锈菌Puccinia recondita f.sp.tritici和禾柄锈菌Puccinia graminis f.sp.tritici全基因组编码蛋白序列,运用生物信息数据库和分析软件对其碳水化合物酶类(CAZymes)基因家族进行了注释和比较分析。结果表明,3种小麦锈菌共包含1 232个CAZymes编码基因(含有1 279个模块)。其中,条形柄锈菌具有明显较大的CAZymes家族,3种锈菌的CAZymes具有136个同源基因;相对于隐匿柄锈菌和禾柄锈菌,条形柄锈菌的GH、GT和AA家族发生显著扩增,而3种锈菌的PL家族并无差异;CBM家族则表现为由禾柄锈菌、隐匿柄锈菌到条形柄锈菌的递增式扩增。通过CAZymes、细胞壁降解酶、分泌蛋白、PHI、保守基序等不同形式注释的比较分析表明,对该基因家族而言,条形柄锈菌相对于隐匿柄锈菌和禾柄锈菌呈扩增现象,隐匿柄锈菌和禾柄锈菌的家族缩减水平存在差异。小麦锈菌CAZymes家族整体呈现的不同形式扩增或缩减现象,可能反映了锈菌与寄主长期协同进化过程中的寄生适应性需求,进而反映了其致病性演化机理。本研究结果有助于进一步全面认识小麦锈菌CAZymes基因家族进化在锈菌致病性变异中的作用,为揭示锈菌致病分子机制提供理论基础。
[Abstract]:In this study, the genomic sequence information of Puccinia striiformis f.sp.tritici, which has been sequenced, was combined with the published genome coding protein sequences of Puccinia recondita f.sp.tritici and Puccinia graminis f.sp.tritici. Bioinformatics database and analysis software were used to annotate and compare the carbohydrate enzyme CAZymes gene family. The results showed that the three wheat rust species contained 1 232 CAZymes coding genes (including 1 279 modules). There were 136 homologous genes in CAZymes of 3 species of rust species of CAZymes family, compared with that of Puccinia occult and Puccinia graminis, the GHGGT and AA families of Puccinia stripeformis were significantly expanded. But the PL family of the three species of rust showed no difference, but the CBM family showed the incremental amplification from Puccinia graminearum, Puccinia occult to Puccinia striatum, and the cell-wall degradation enzyme was obtained by CAZymes. The comparative analysis of the secretory protein PHIand conserved motifs showed that, for the gene family, Puccinia striatum was more abundant than Puccinia occult and Puccinia graminearum. There were differences in the family reduction level between Puccinia occult and Puccinia graminis. The different forms of expansion or reduction in the CAZymes family of wheat rust might reflect the parasitic adaptability during the long-term coevolution of rust and host. The results of this study are helpful to further understand the role of CAZymes gene family evolution in the pathogenicity variation of wheat rust, and provide a theoretical basis for revealing the molecular mechanism of rust pathogen pathogenicity.
【作者单位】：河南农业大学生命科学学院小麦玉米作物学国家重点实验室河南粮食作物协同创新中心;西北农林科技大学植物保护学院旱区作物逆境生物学国家重点实验室;
【基金】：国家重点基础研究发展计划(2013CB127702) 河南省科技创新杰出人才计划(154200510024) 河南省重大科技专项(161100110400)~~
【分类号】：S435.12

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