舞毒蛾小分子热激蛋白基因分析及对甲萘威胁迫的响应

发布时间：2018-03-09 18:01

本文选题：舞毒蛾　切入点：smHsp　出处：《北京林业大学学报》2017年01期 　论文类型：期刊论文

【摘要】：昆虫小分子热激蛋白(smHsp)是分子质量在12~43 k Da、具有分子伴侣活性的应激蛋白,可以在环境胁迫下帮助蛋白质折叠和转运。本文为明确舞毒蛾的smHsp基因特性及对杀虫剂甲萘威胁迫的响应,从舞毒蛾无参照全转录本文库中鉴定获得6个小分子热激蛋白家族基因,全长基因开放阅读框为444~567 bp,编码147~188个氨基酸,理论等电点为5.58~6.17。根据分子质量大小分别命名为Ld Hsp17.0、Ld Hsp18.7、Ld Hsp19.1、Ld Hsp20.3、Ld Hsp21.3和Ld Hsp21.4。亚致死剂量(LC_5、LC_(10)和LC_(30))的甲萘威处理可诱导舞毒蛾smHsp家族中Ld Hsp20.3、Ld Hsp19.1和Ld Hsp17.0上调表达,但抑制了Ld Hsp21.4、Ld Hsp21.3和Ld Hsp18.7的表达,且甲萘威处理对smHsp基因表达量的影响存在质量浓度和剂量效应。以上结果为进一步研究舞毒蛾小分子热激蛋白功能及其参与对杀虫剂抗性的机制提供了基础。
[Abstract]:Insect small molecular heat shock protein (SMP) is a stress protein with molecular weight at 124K Da. it has molecular chaperone activity. It can help protein folding and transport under environmental stress. In this paper, the characteristics of smHsp gene and its response to the insecticide Carbaryl stress were determined. Six small molecular heat shock protein family genes were identified from the whole transcription library without reference. The open reading frame of the full-length gene was 444 ~ 567 BP, encoding 147-188 amino acids. The theoretical isoelectric point is 5.58 ~ 6.17. According to the molecular weight of Ld Hsp17.0, Ld Hsp18.7n Ld Hsp19.1UP Ld Hsp20.3 Ld Hsp21.3 and Ld Hsp21.4Ld Hsp20.3 Ld Hsp21.3 and Ld Hsp21.4Ld Hsp20.3Ld Hsp19.1 and Ld Hsp17.0 can be induced to express Ld Hsp20.3Ld Hsp19.1 and Ld Hsp17.0 in the smHsp family. However, the expression of Ld Hsp21.3 and Ld Hsp18.7 in LdHsp21.4 was inhibited. The effect of carbaryl treatment on the expression of smHsp gene has a concentration and dose effect, which provides a basis for further study on the function of small molecular heat shock protein and its mechanism involved in insecticide resistance.
【作者单位】：东北林业大学林学院;
【基金】：国家自然科学基金项目(31570642、31101676) 黑龙江省自然科学基金面上项目(C201409)
【分类号】：S763.42

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