环尾狐猴源弓形虫多位点基因的克

发布时间：2018-03-09 19:12

本文选题：刚地弓形虫　切入点：基因分型　出处：《野生动物学报》2017年02期 　论文类型：期刊论文

【摘要】：为确定引起广东环尾狐猴(Lemur catta)疑似弓形虫病的病原,从2只死亡的环尾狐猴体内无菌采集肺脏、肝脏、心、肾和脑等组织制作常规石蜡切片,HE染色。通过病理组织学观察,鉴定为弓形虫(Toxoplasma sp.)后,采用PCR-DNA测序分析方法对环尾狐猴源分离虫株(TgRTL1)的SAG1、SAG2、SAG3、BTUB、GRA6、L358、PK1、c22-8、c29-2和Apico基因位点进行PCR扩增、克隆和测序,采用DNAStar和MEGA5.05软件,将所获序列与网上下载的弓形虫RH株、ME49株和VEG株相应序列进行比对和同源性分析,并构建系统发育树。结果显示,从广东分离的环尾狐猴源弓形虫株,在10个遗传位点经PCR-DNA测序分析,确定该虫株的基因型为Toxo DB#3。本研究是对中国环尾狐猴源弓形虫采用多位点DNA序列分析进行基因型鉴定的首次报道,为进一步研究我国弓形虫流行病学、生物学特性以及弓形虫病的诊断提供了科学理论依据。
[Abstract]:In order to determine the pathogen of the suspected toxoplasmosis, lung, liver, heart, kidney and brain tissues were collected from two dead lemurs to make routine paraffin sections and HE staining. After being identified as Toxoplasma sp.), SAG1, SAG2 and SAG3 of TgRTL1) were amplified by PCR, cloned and sequenced by PCR, cloned and sequenced by DNAStar and MEGA5.05 software. The sequence was compared with the corresponding sequences of Toxoplasma gondii RH strain ME49 and VEG strain, and the phylogenetic tree was constructed. The genotypes of Toxoplasma gondii were determined to be Toxo DB#3 by PCR-DNA sequencing analysis at 10 genetic loci. This study was the first report to identify the genotypes of Toxoplasma gondii (Toxoplasma gondii) by multilocus DNA sequence analysis. It provides scientific theoretical basis for further study on epidemiology, biological characteristics and diagnosis of Toxoplasma gondii in China.
【作者单位】：华南农业大学;上海市野生动物园有限公司;广州动物园;
【分类号】：S858.9

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