FOXL2基因与蛋鸡卵巢发育的相关性研究

发布时间：2018-03-11 15:11

  本文选题：FOXL基因　切入点：海兰褐蛋鸡　出处：《中国家禽》2017年10期 　论文类型：期刊论文

【摘要】：试验采集120、160、220、330、480日龄海兰褐蛋鸡的卵巢,通过石蜡切片观察卵泡发育情况,采用荧光定量PCR检测FOXL2基因在卵巢的表达情况。结果显示:120~480日龄,卵巢重量呈现先增加后减少的趋势,220日龄达到最大;卵巢的初级卵泡数逐渐减少,而次级卵泡数先增加后减少,160日龄次级卵泡数最多;蛋鸡卵泡萎缩率增加,闭锁卵泡数逐渐增多;FOXL2基因的m RNA水平在120~480日龄呈下降趋势,暗示其可能与蛋鸡卵巢和卵泡的发育存在相关性。
[Abstract]:The ovaries of 120,160,220,330,480 day-old Hailan brown laying hens were collected and the follicle development was observed by paraffin section. The expression of FOXL2 gene in ovary was detected by fluorescence quantitative PCR. The weight of ovary increased first and then decreased. The number of primary follicles decreased gradually, while the number of secondary follicles increased first and then decreased at 160 days of age. The rate of follicle atrophy increased. The number of atretic follicles increased gradually and the m RNA level of FOXL2 gene decreased at 120-480 days, suggesting that it might be related to the development of ovaries and follicles in laying hens.
【作者单位】： 河南科技学院动物科技学院;
【基金】：河南省基础与前沿技术研究计划项目(132300410014)
【分类号】：S831.2
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本文编号：1598712