孕前应激诱导的产后抑郁模型小鼠海马精神分裂症断裂基因1的表达变化

发布时间：2018-03-11 16:25

  本文选题：产后抑郁　切入点：孕前应激　出处：《中国医学科学院学报》2017年02期 　论文类型：期刊论文

【摘要】：目的以孕前应激诱导方式建立产后抑郁症(PPD)小鼠模型,评估母鼠产后的异常行为,检测母鼠海马精神分裂症断裂基因1(DISC1)及血清雌二醇、皮质酮的变化,揭示产后抑郁的发病机制。方法将32只Balb/c母鼠采用随机数字表法分为对照组和模型组,每组16只,对照组小鼠不给予任何刺激,模型组小鼠给予慢性束缚刺激。3周后对照组和模型组均予雌雄合笼交配怀孕,分娩后即为对照组和孕前应激诱导PPD模型组。于分娩后3周对母鼠进行旷场测试、悬尾测试及糖水偏爱测试以评价造模是否成功。取海马检测DISC1基因及蛋白的表达,眼眶取血检测血清雌二醇及皮质酮水平。结果产后3周,与对照组相比,PPD模型组在旷场测试中自发活动无改变(P0.05),在悬尾测试中的绝望不动时间显著增加(t=-4.950,P0.001),糖水偏爱显著降低(t=2.475,P0.05)。与对照组相比,PPD模型组海马DISC1基因(t=-8.915,P0.001)及蛋白(t=-5.004,P0.01)表达均显著上调,而血清皮质酮及雌二醇均无变化(P均0.05)。结论慢性孕前应激可诱导Balb/c母鼠表现出产后抑郁样症状,其发病机制可能与调控海马DISC1的基因和蛋白表达有关。
[Abstract]:Objective to establish the model of postpartum depression (PPD) mice by pre-pregnancy stress, to evaluate the abnormal behavior of postpartum rats, and to detect the changes of estradiol and corticosterone in the serum of the female rats. Methods 32 Balb/c mice were randomly divided into control group (n = 16) and model group (n = 16). The model group mice were given chronic restraint stimulation for 3 weeks, the control group and the model group were given female and male cage mating pregnancy, then the control group and the pre-pregnancy stress induced PPD model group. The open field test was carried out on the female mice 3 weeks after delivery. Tail suspension test and sugar water preference test were used to evaluate the success of the model. The expression of DISC1 gene and protein was detected in hippocampus, serum estradiol and corticosterone levels were detected in orbital blood samples. Compared with the control group, the spontaneous activity of PPD model group did not change in the open field test, but the desperate immobility time in the tail suspension test increased significantly (P 0.001), and the sugar water preference decreased significantly. Compared with the control group, the hippocampal DISC1 gene t-8.915P0.001 and the protein were significantly decreased in the PPD model group. The expression of TG-5.004 was significantly up-regulated, and the expression of P0.01 was significantly up-regulated. Serum corticosterone and estradiol had no change (P 0.05). Conclusion chronic prepregnancy stress can induce postpartum depressive symptoms in Balb/c rats, and its pathogenesis may be related to the regulation of gene and protein expression of hippocampal DISC1.
【作者单位】： 南京特殊教育师范学院康复科学学院医学康复教研室;南京中医药大学基础医学院脑病重点实验室;南京中医药大学第三附属医院脑病科;
【基金】：江苏省自然科学基金(BK20151568)~~
【分类号】：R749;R-332
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本文编号：1598957