丝切蛋白CFL2基因低表达对小鼠成肌细胞C2C12肌纤维信号通路关键成分的影响

发布时间：2018-03-24 21:28

本文选题：丝切蛋白　切入点：CFL　出处：《畜牧与兽医》2017年03期

【摘要】：探讨低表达丝切蛋白CFL2基因对小鼠成肌细胞C2C12信号通路关键成分的影响。采用RNAi技术,将猪CFL2基因真核表达shRNA重组体稳定转染至小鼠成肌细胞C2C12后提取其总RNA和总蛋白质,采用Real-time PCR和Western blot检测CFL2基因低表达后成肌细胞C2C12信号通路关键成分(MEF2C、sk MLCK、Rho A、AMPKα2、p38和Ca M)的表达情况。结果表明:在3个稳转细胞株中MEF2C、Ca M、p38和AMPKα2的mRNA表达均为极显著下调(P0.01),sk MLCK的mRNA表达为极显著上调(P0.01),其它成分不显著;MEF2C和Ca M的蛋白表达在3个稳转细胞株中均为显著或极显著下调(P0.05或P0.01),其他4种成分(p38、Rho A、AMPKα2和sk MLCK)均差异不显著(P0.05),说明CFL2低表达显著下调信号通路关键成分MEF2C和Ca M的表达,CFL2与Ca M和sk MLCK分别呈显著正相关和负相关。
[Abstract]:To investigate the effect of low expression of CFL2 gene on the key components of C2C12 signaling pathway in mouse myoblasts, the porcine CFL2 gene eukaryotic expression shRNA recombinant was stably transfected into mouse myoblast C2C12 to extract its total RNA and total protein by RNAi technique. Real-time PCR and Western blot were used to detect the expression of the key components of C2C12 signal pathway in myoblasts after low expression of CFL2 gene. The results showed that the mRNA expression of MEF2CU Ca Mp38 and AMPK 伪 2 were significantly down-regulated in three stable cell lines. The mRNA expression of P0.01 MLCK was significantly up-regulated, while the protein expression of MEF2C and Cam was significantly or extremely down-regulated in all of the three stable cell lines, while the other four components, p38, Rho, AMPK 伪 2, and SK MLCK, had no significant difference in the expression of P0.05, said P0.05, saying that the expression of MEF2C and Cam in the three stable cell lines was significantly or extremely down-regulated by P0.01 or P0.01a, and that there was no significant difference between the other four components, P38, Rho, AMPK 伪 2 and SK MLCKs, said. The low expression of CFL2 down-regulated the expression of MEF2C and Cam. The expression of CFL2 was positively and negatively correlated with Cam and SK MLCK, respectively.
【作者单位】：锦州医科大学畜牧兽医学院;辽宁省畜产品质量与安全工程重点实验室;
【基金】：国家自然科学基金(31272415/C170102)
【分类号】：Q78

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