普城沙雷氏菌pigX基因的生物信息学分析

发布时间：2018-03-24 21:37

本文选题：普城沙雷氏菌　切入点：GGDEF/EAL结构域　出处：《江苏农业学报》2017年03期

【摘要】：通过生物信息学方法对普城沙雷氏菌(Serratia plymuthica)G3菌株中GGDEF/EAL结构域蛋白编码基因pigX启动子区域的调控信息以及编码蛋白质的理化特性和结构特点进行了解析。利用启动子分析软件Softberry和Neural Network Promoter Prediction对pigX基因编码区上游序列进行启动子预测,利用生物信息学方法对PigX蛋白的氨基酸组成和理化特性、磷酸化和糖基化位点、跨膜结构、信号肽二级结构和保守结构域等进行预测分析。结果显示:pigX启动子区有Fur和SoxS结合位点;PigX是微亲水性的脂结合蛋白,具有信号肽和跨膜结构域、糖基化和高水平磷酸化位点,α-螺旋和无规则卷曲是其主要二级结构。生物信息学分析预测结果表明G3菌株PigX具有较高水平磷酸化位点及由α-螺旋和无规则卷曲组成的二级结构,这与预测的PigX参与铁代谢和氧化应激反应及作为Csr D同源物与CSRB家族sRNAs分子结合的功能相吻合。
[Abstract]:The regulation information of the promoter region of GGDEF/EAL domain protein encoding gene pigX in Serratia plymuthica)G3 strain was analyzed by bioinformatics. The physicochemical and structural characteristics of the encoded protein were analyzed by using the promoter. Softberry and Neural Network Promoter Prediction were used to predict the upstream sequence of pigX gene. The amino acid composition, physicochemical properties, phosphorylation and glycosylation sites, transmembrane structure of PigX protein were studied by bioinformatics. The signal peptide secondary structure and conserved domain were predicted and analyzed. The results showed that Fur and SoxS binding sites were found to be microhydrophilic lipopolysaccharide binding proteins, with signal peptide and transmembrane domain. Glycosylation and high level phosphorylation sites, 伪 -helix and irregular crimp were the main secondary structures. Bioinformatics analysis and prediction showed that G3 strain PigX had higher phosphorylation sites and secondary structures consisting of 伪 -helix and irregular crimp. This is consistent with the predicted function of PigX involved in iron metabolism and oxidative stress reaction and binding of Csr D homologues to CSRB family sRNAs molecules.
【作者单位】：江苏大学食品与生物工程学院;江苏大学生命科学研究院;
【基金】：国家自然科学基金项目(31240046) 公益性行业(农业)科研专项(201503110-12)
【分类号】：Q811.4;S476.1

【相似文献】